Isolation:Article Title: Universal STING mimic boosts antitumour immunity via preferential activation of tumour control signalling pathways
Article Snippet: miRNAs were extracted from EVs using an miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. .. The isolated RNAs were converted into cDNA by using the miScript II reverse transcription reaction with HiSpec Buffer (Qiagen). cDNA was used as the template in qPCR with the miScript miRNA PCR Array Mouse Inflammatory Response & Autoimmunity and miScript SYBR Green PCR Kit (Qiagen). qPCR was performed on a Thermal Cycler ABI9700 and QuantStudio 6 Flex Real-Time PCR system using the following thermocycling parameters: 94 °C for 15 min, 40 cycles at 94 °C for 10 s, 55 °C for 30 s and 70 °C for 30 s, followed by a melting curve analysis with QuantStudio software v.1.7.2 (Applied Biosystems). .. The data were further analysed by using the online miRNA PCR array data analysis tool ( www.qiagen.com/us/shop/pcr/primer-sets/miscript-mirna-pcr-arrays/#resources ) according to the manufacturer’s instructions. miRNAs with a threshold cycle value >35 were considered as undetermined to minimize the potential noise introduced by measurements below the detection threshold.
Reverse Transcription:Article Title: Universal STING mimic boosts antitumour immunity via preferential activation of tumour control signalling pathways
Article Snippet: miRNAs were extracted from EVs using an miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. .. The isolated RNAs were converted into cDNA by using the miScript II reverse transcription reaction with HiSpec Buffer (Qiagen). cDNA was used as the template in qPCR with the miScript miRNA PCR Array Mouse Inflammatory Response & Autoimmunity and miScript SYBR Green PCR Kit (Qiagen). qPCR was performed on a Thermal Cycler ABI9700 and QuantStudio 6 Flex Real-Time PCR system using the following thermocycling parameters: 94 °C for 15 min, 40 cycles at 94 °C for 10 s, 55 °C for 30 s and 70 °C for 30 s, followed by a melting curve analysis with QuantStudio software v.1.7.2 (Applied Biosystems). .. The data were further analysed by using the online miRNA PCR array data analysis tool ( www.qiagen.com/us/shop/pcr/primer-sets/miscript-mirna-pcr-arrays/#resources ) according to the manufacturer’s instructions. miRNAs with a threshold cycle value >35 were considered as undetermined to minimize the potential noise introduced by measurements below the detection threshold.
Real-time Polymerase Chain Reaction:Article Title: Universal STING mimic boosts antitumour immunity via preferential activation of tumour control signalling pathways
Article Snippet: miRNAs were extracted from EVs using an miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. .. The isolated RNAs were converted into cDNA by using the miScript II reverse transcription reaction with HiSpec Buffer (Qiagen). cDNA was used as the template in qPCR with the miScript miRNA PCR Array Mouse Inflammatory Response & Autoimmunity and miScript SYBR Green PCR Kit (Qiagen). qPCR was performed on a Thermal Cycler ABI9700 and QuantStudio 6 Flex Real-Time PCR system using the following thermocycling parameters: 94 °C for 15 min, 40 cycles at 94 °C for 10 s, 55 °C for 30 s and 70 °C for 30 s, followed by a melting curve analysis with QuantStudio software v.1.7.2 (Applied Biosystems). .. The data were further analysed by using the online miRNA PCR array data analysis tool ( www.qiagen.com/us/shop/pcr/primer-sets/miscript-mirna-pcr-arrays/#resources ) according to the manufacturer’s instructions. miRNAs with a threshold cycle value >35 were considered as undetermined to minimize the potential noise introduced by measurements below the detection threshold.
Polymerase Chain Reaction:Article Title: Universal STING mimic boosts antitumour immunity via preferential activation of tumour control signalling pathways
Article Snippet: miRNAs were extracted from EVs using an miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. .. The isolated RNAs were converted into cDNA by using the miScript II reverse transcription reaction with HiSpec Buffer (Qiagen). cDNA was used as the template in qPCR with the miScript miRNA PCR Array Mouse Inflammatory Response & Autoimmunity and miScript SYBR Green PCR Kit (Qiagen). qPCR was performed on a Thermal Cycler ABI9700 and QuantStudio 6 Flex Real-Time PCR system using the following thermocycling parameters: 94 °C for 15 min, 40 cycles at 94 °C for 10 s, 55 °C for 30 s and 70 °C for 30 s, followed by a melting curve analysis with QuantStudio software v.1.7.2 (Applied Biosystems). .. The data were further analysed by using the online miRNA PCR array data analysis tool ( www.qiagen.com/us/shop/pcr/primer-sets/miscript-mirna-pcr-arrays/#resources ) according to the manufacturer’s instructions. miRNAs with a threshold cycle value >35 were considered as undetermined to minimize the potential noise introduced by measurements below the detection threshold.
SYBR Green Assay:Article Title: Universal STING mimic boosts antitumour immunity via preferential activation of tumour control signalling pathways
Article Snippet: miRNAs were extracted from EVs using an miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. .. The isolated RNAs were converted into cDNA by using the miScript II reverse transcription reaction with HiSpec Buffer (Qiagen). cDNA was used as the template in qPCR with the miScript miRNA PCR Array Mouse Inflammatory Response & Autoimmunity and miScript SYBR Green PCR Kit (Qiagen). qPCR was performed on a Thermal Cycler ABI9700 and QuantStudio 6 Flex Real-Time PCR system using the following thermocycling parameters: 94 °C for 15 min, 40 cycles at 94 °C for 10 s, 55 °C for 30 s and 70 °C for 30 s, followed by a melting curve analysis with QuantStudio software v.1.7.2 (Applied Biosystems). .. The data were further analysed by using the online miRNA PCR array data analysis tool ( www.qiagen.com/us/shop/pcr/primer-sets/miscript-mirna-pcr-arrays/#resources ) according to the manufacturer’s instructions. miRNAs with a threshold cycle value >35 were considered as undetermined to minimize the potential noise introduced by measurements below the detection threshold.
Software:Article Title: Universal STING mimic boosts antitumour immunity via preferential activation of tumour control signalling pathways
Article Snippet: miRNAs were extracted from EVs using an miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. .. The isolated RNAs were converted into cDNA by using the miScript II reverse transcription reaction with HiSpec Buffer (Qiagen). cDNA was used as the template in qPCR with the miScript miRNA PCR Array Mouse Inflammatory Response & Autoimmunity and miScript SYBR Green PCR Kit (Qiagen). qPCR was performed on a Thermal Cycler ABI9700 and QuantStudio 6 Flex Real-Time PCR system using the following thermocycling parameters: 94 °C for 15 min, 40 cycles at 94 °C for 10 s, 55 °C for 30 s and 70 °C for 30 s, followed by a melting curve analysis with QuantStudio software v.1.7.2 (Applied Biosystems). .. The data were further analysed by using the online miRNA PCR array data analysis tool ( www.qiagen.com/us/shop/pcr/primer-sets/miscript-mirna-pcr-arrays/#resources ) according to the manufacturer’s instructions. miRNAs with a threshold cycle value >35 were considered as undetermined to minimize the potential noise introduced by measurements below the detection threshold.
|
Qiagen is a verified supplier 
Qiagen manufactures this product 