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5 × miscript hispec buffer  (Qiagen)


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    Structured Review

    Qiagen 5 × miscript hispec buffer
    5 × Miscript Hispec Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+hispec+buffer/5%C3%97+miscript+hispec+buffer/pmc11607072-229-9-12
    Average 90 stars, based on 1 article reviews
    5 × miscript hispec buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Evaluation of tumorigenesis-related miRNAs in breast cancer in Egyptian women: a retrospective, exploratory analysis
    Article Snippet: The reaction mix included 4 μl of 5 × miScript HiSpec Buffer (Qiagen, Germany), 2 μl of 10 × miScript Nucleics Mix (Qiagen, Germany), 2 μl of miScript RT Mix (Qiagen, Germany), 60 ng of Template RNA, and RNase-free water was added to bring it to the desired volume.

    Article Title: Electrodeposited Magnetic Nanoporous Membrane (MNM) with a Multi-Edge Superparamagnetic Heterogeneous Wedge Junction for High-Yield and High-Throughput Immunocapture of Specific Extracellular Vesicles and Lipoproteins
    Article Snippet: 338 A 20 μL reverse transcription reaction was prepared with 2.2 μL of eluted miRNA, 4 μL 5× miScript 339 HiSpec Buffer (Qiagen), 2 μL 10× miScript Nucleics Mix (Qiagen), 9.8 μL RNase-free water, and 2 μL 340 miScript Reverse Transcriptase Mix (Qiagen).

    Article Title: Electrodeposited Magnetic Nanoporous Membrane (MNM) with a Multi-Edge Superparamagnetic Heterogeneous Wedge Junction for High-Yield and High-Throughput Immunocapture of Specific Extracellular Vesicles and Lipoproteins
    Article Snippet: 338 A 20 μL reverse transcription reaction was prepared with 2.2 μL of eluted miRNA, 4 μL 5× miScript 339 HiSpec Buffer (Qiagen), 2 μL 10× miScript Nucleics Mix (Qiagen), 9.8 μL RNase-free water, and 2 μL 340 miScript Reverse Transcriptase Mix (Qiagen).

    Reverse Transcription:

    Article Title: A Bifurcated Continuous Field-Flow Fractionation (BCFFF) Chip for High-Yield and High-Throughput Nucleic Acid Extraction and Purification
    Article Snippet: Reverse transcription was carried out using a miScript II RT Kit (Qiagen). .. A 20 μL reverse transcription reaction was prepared with 2μL of eluted miRNA, 4μL 5× miScript HiSpec Buffer (Qiagen), 2μL 10× miScript Nucleics Mix (Qiagen), 10μL RNase-free water, and 2μL miScript Reverse Transcriptase Mix (Qiagen). .. Reverse transcription was carried out using a miScript II RT Kit (Qiagen).

    Article Title: LncRNA EGFR‑AS1 promotes lung cancer cell proliferation, invasion and metastasis via regulation of miR‑449a/HDAC1
    Article Snippet: The cDNA synthesis was carried out based on the instructions of the miScript II RT Kit (Qiagen). .. Briefly, 1 μg total RNA was added to 12 μl DEPC treated water, 2 μl miScript Reverse Transcriptase Mix (Qiagen GmbH), 2 μl miScript Nucleics Mix (Qiagen GmbH) and 4 μl 5x miScript HiSpec buffer (Qiagen GmbH). ..

    Article Title: Comprehensive assessment of circulatory miRNAs as potential diagnostic markers in HCV recurrence post liver transplantation.
    Article Snippet: HCV recurrence after liver transplantation is one of the causal agents for graft rejection.. This study aims to profile non-invasive biomarkers in patients with HCC who had liver transplants.. One hundred participants were categorized into three groups (20 control, 32 recurrent HCV (RHCV), and 48 non-RHCV).

    Article Title: Electrodeposited magnetic nanoporous membrane for high-yield and high-throughput immunocapture of extracellular vesicles and lipoproteins
    Article Snippet: Reverse transcription was carried out using a miScript II RT Kit (Qiagen). .. A 20 μL reverse transcription reaction was prepared with 2.2 μL of eluted miRNA, 4 μL 5 × miScript HiSpec Buffer (Qiagen), 2 μL 10 × miScript Nucleics Mix (Qiagen), 9.8 μL RNase-free water, and 2 μL miScript Reverse Transcriptase Mix (Qiagen). ..

    Article Title: Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts
    Article Snippet: 178 179 RT-qPCR analysis 180 Total RNA samples (including miRNAs) were isolated from embryos (three replicates of 8 embryos each) 181 using the miRNeasy Mini kit (Qiagen, Germantown, USA). .. Reverse transcription was performed in 20 182 μl reaction volume on a PCR system using a miScript II RT kit (Qiagen, Germantown, USA), including 183 4 μl 5 × miScript Hispec Buffer, 2 μl 10 × miScript Nucleics Mix, 2 μl miScript Reverse Transcriptase Mix 184 and 12 μl RNA. ..

    Polymerase Chain Reaction:

    Article Title: Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts
    Article Snippet: 178 179 RT-qPCR analysis 180 Total RNA samples (including miRNAs) were isolated from embryos (three replicates of 8 embryos each) 181 using the miRNeasy Mini kit (Qiagen, Germantown, USA). .. Reverse transcription was performed in 20 182 μl reaction volume on a PCR system using a miScript II RT kit (Qiagen, Germantown, USA), including 183 4 μl 5 × miScript Hispec Buffer, 2 μl 10 × miScript Nucleics Mix, 2 μl miScript Reverse Transcriptase Mix 184 and 12 μl RNA. ..



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