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mirna labeling, quality control, chip hybridization and microarray analysis  (CapitalBio Corporation)

 
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    Structured Review

    CapitalBio Corporation mirna labeling, quality control, chip hybridization and microarray analysis
    Mirna Labeling, Quality Control, Chip Hybridization And Microarray Analysis, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirna+microarray+data+analysis/mirna+labeling++quality+control++chip+hybridization+and+microarray+analysis/pmc05154343-155-17-5
    Average 90 stars, based on 1 article reviews
    mirna labeling, quality control, chip hybridization and microarray analysis - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: MiR-125a promotes paclitaxel sensitivity in cervical cancer through altering STAT3 expression
    Article Snippet: Total RNA was extracted from HeLa and CaSki cells and their paclitaxel-resistant counterparts using a Qiagen miRNeasy mini kit and following the manufacturer protocol. .. Total RNA was sent to CapitalBio Corporation (Beijing, China) for miRNA labeling, quality control, chip hybridization and microarray analysis. ..

    Article Title: miR-30a-5p enhances paclitaxel sensitivity in non-small cell lung cancer through targeting BCL-2 expression.
    Article Snippet: Lung cancer remains the leading cause of cancer-related death worldwide.. Paclitaxel, either as monotherapy or combined with other agents, is the standard treatment for advanced non-small cell lung cancer (NSCLC), the most common type of lung cancer.. However, both de novo and acquired resistance against paclitaxel frequently occurs and represents a huge clinical problem.

    Control:

    Article Title: MiR-125a promotes paclitaxel sensitivity in cervical cancer through altering STAT3 expression
    Article Snippet: Total RNA was extracted from HeLa and CaSki cells and their paclitaxel-resistant counterparts using a Qiagen miRNeasy mini kit and following the manufacturer protocol. .. Total RNA was sent to CapitalBio Corporation (Beijing, China) for miRNA labeling, quality control, chip hybridization and microarray analysis. ..

    Article Title: miR-30a-5p enhances paclitaxel sensitivity in non-small cell lung cancer through targeting BCL-2 expression.
    Article Snippet: Lung cancer remains the leading cause of cancer-related death worldwide.. Paclitaxel, either as monotherapy or combined with other agents, is the standard treatment for advanced non-small cell lung cancer (NSCLC), the most common type of lung cancer.. However, both de novo and acquired resistance against paclitaxel frequently occurs and represents a huge clinical problem.

    Hybridization:

    Article Title: MiR-125a promotes paclitaxel sensitivity in cervical cancer through altering STAT3 expression
    Article Snippet: Total RNA was extracted from HeLa and CaSki cells and their paclitaxel-resistant counterparts using a Qiagen miRNeasy mini kit and following the manufacturer protocol. .. Total RNA was sent to CapitalBio Corporation (Beijing, China) for miRNA labeling, quality control, chip hybridization and microarray analysis. ..

    Article Title: miR-30a-5p enhances paclitaxel sensitivity in non-small cell lung cancer through targeting BCL-2 expression.
    Article Snippet: Lung cancer remains the leading cause of cancer-related death worldwide.. Paclitaxel, either as monotherapy or combined with other agents, is the standard treatment for advanced non-small cell lung cancer (NSCLC), the most common type of lung cancer.. However, both de novo and acquired resistance against paclitaxel frequently occurs and represents a huge clinical problem.

    Microarray:

    Article Title: MiR-125a promotes paclitaxel sensitivity in cervical cancer through altering STAT3 expression
    Article Snippet: Total RNA was extracted from HeLa and CaSki cells and their paclitaxel-resistant counterparts using a Qiagen miRNeasy mini kit and following the manufacturer protocol. .. Total RNA was sent to CapitalBio Corporation (Beijing, China) for miRNA labeling, quality control, chip hybridization and microarray analysis. ..

    Article Title: miR-30a-5p enhances paclitaxel sensitivity in non-small cell lung cancer through targeting BCL-2 expression.
    Article Snippet: Lung cancer remains the leading cause of cancer-related death worldwide.. Paclitaxel, either as monotherapy or combined with other agents, is the standard treatment for advanced non-small cell lung cancer (NSCLC), the most common type of lung cancer.. However, both de novo and acquired resistance against paclitaxel frequently occurs and represents a huge clinical problem.



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    Image Search Results


    (A)Volcano plot of miRNA expression in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.

    Journal: Circulation. Heart failure

    Article Title: Differential microRNA-21 and microRNA-221 upregulation in the biventricular failing heart reveals distinct stress responses of right versus left ventricular fibroblasts

    doi: 10.1161/CIRCHEARTFAILURE.119.006426

    Figure Lengend Snippet: (A)Volcano plot of miRNA expression in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.

    Article Snippet: miRNA microarray data was analyzed for differential miRNA expression between pre-specified groups (RV-HF vs. RV-Ctrl, LV-HF vs. LV-Ctrl, and RV-HF vs. LV-HF) by two-tailed Student’s t-test using GraphPad Software (Prism 7.0).

    Techniques: Expressing, Labeling, Quantitative RT-PCR, Inhibition