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mirna microarray analysis  (Phalanx Biotech)

 
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    Phalanx Biotech mirna microarray analysis
    Mirna Microarray Analysis, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirna+microarray+data+analysis/mirna+microarray+analysis/pmc05408584-258-4-7
    Average 90 stars, based on 1 article reviews
    mirna microarray analysis - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Modification:

    Article Title: microRNA biosensors: Opportunities and challenges among conventional and commercially available techniques.
    Article Snippet: As being the most extensively studied, non-coding, evolutionary conserved, posttranscriptional gene regulators of genome, microRNAs (miRNAs) have taken great attention among various disciplines due to their important roles in biological processes and link with cancer.. Due to their diagnostic value, there have been many conventional methods used in detection of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR) and microarray technology besides novel techniques based on various nanotechnology approaches and molecular biology tools including miRNA biosensors.. The aim of this review is to explain the importance of miRNAs in biomedical field with an emphasis on early cancer diagnosis by overviewing both research based and commercially available miRNA detection methods in the last decade considering their strengths and weakness with an emphasis on miRNA biosensors.

    Knockdown:

    Article Title: DDX 3 participates in mi RNA biogenesis and RNA interference through translational control of PACT and interaction with AGO 2
    Article Snippet: .. To identify miRNAs regulated by DDX3, we used OneArray miRNA Microarrays (Phalanx Biotech Group, Hsinchu, Taiwan) to screen differentially expressed miRNAs in DDX3 knockdown HEK293T cells compared to control cells. ..

    Article Title: DDX3 participates in miRNA biogenesis and RNA interference through translational control of PACT and interaction with AGO2.
    Article Snippet: .. Depletion of DDX3 affects the expression of a small subset of cancer-related miRNAs in HEK293T cells To identify miRNAs regulated by DDX3, we used OneArray miRNA Microarrays (Phalanx Biotech Group, Hsinchu, Taiwan) to screen differentially expressed miRNAs in DDX3 knockdown HEK293T cells compared to control cells. ..

    Control:

    Article Title: DDX 3 participates in mi RNA biogenesis and RNA interference through translational control of PACT and interaction with AGO 2
    Article Snippet: .. To identify miRNAs regulated by DDX3, we used OneArray miRNA Microarrays (Phalanx Biotech Group, Hsinchu, Taiwan) to screen differentially expressed miRNAs in DDX3 knockdown HEK293T cells compared to control cells. ..

    Article Title: DDX3 participates in miRNA biogenesis and RNA interference through translational control of PACT and interaction with AGO2.
    Article Snippet: .. Depletion of DDX3 affects the expression of a small subset of cancer-related miRNAs in HEK293T cells To identify miRNAs regulated by DDX3, we used OneArray miRNA Microarrays (Phalanx Biotech Group, Hsinchu, Taiwan) to screen differentially expressed miRNAs in DDX3 knockdown HEK293T cells compared to control cells. ..

    Article Title: Dicer Elicits Paclitaxel Chemosensitization and Suppresses Cancer Stemness in Breast Cancer by Repressing AXL
    Article Snippet: .. The transfectants were grown under the same conditions as the control, except that G418 was added to the culture medium. miRNA microarray hybridization 2.5 μg of total RNA obtained from MDA-MB-231/vector, MDA-MB-231/E1A and MDA-MB-231/E1A/shDicer cells were labeled and hybridized on miRNA microarrays (using the Human miRNA OneArray® v2 (Phalanx Biotech Group, San Diego, CA, USA). ..

    Microarray:

    Article Title: MiR-125a-5p promotes osteoclastogenesis by targeting TNFRSF1B
    Article Snippet: .. MiRNA microarray assay was completed by Phalanx Biotech Group (Xinzhu, Taiwan, China). ..

    Article Title: Fatty acid binding protein-1 (FABP1) and the human FABP1 T94A Variant: Roles in the Endocannabinoid System and Dyslipidemias
    Article Snippet: .. Consistent with this finding, miRNA microarray analysis (Phalanx Biotech Group (San Diego, CA) reveals that T94A increases the level of miR-34a (not shown). miR34a decreases the protein level of CPT1A (rate-limiting enzyme in mitochondrial LCFA β-oxidation) ( 210 ) and miR-34a is highly increased in human NAFLD ( 210 , 211 ). ..

    Article Title: Fatty Acid Binding Protein-1 (FABP1) and the Human FABP1 T94A Variant: Roles in the Endocannabinoid System and Dyslipidemias.
    Article Snippet: .. Consistent with this finding, miRNA microarray analysis (Phalanx Biotech Group, San Diego, CA) reveals that T94A increases the level of miR-34a (not shown). miR34a decreases the protein level of CPT1A (rate-limiting enzyme in mitochondrial LCFA β-oxidation) [210] and miR-34a is highly increased in human NAFLD [210, 211]. ..

    Expressing:

    Article Title: DDX3 participates in miRNA biogenesis and RNA interference through translational control of PACT and interaction with AGO2.
    Article Snippet: .. Depletion of DDX3 affects the expression of a small subset of cancer-related miRNAs in HEK293T cells To identify miRNAs regulated by DDX3, we used OneArray miRNA Microarrays (Phalanx Biotech Group, Hsinchu, Taiwan) to screen differentially expressed miRNAs in DDX3 knockdown HEK293T cells compared to control cells. ..

    Hybridization:

    Article Title: Dicer Elicits Paclitaxel Chemosensitization and Suppresses Cancer Stemness in Breast Cancer by Repressing AXL
    Article Snippet: .. The transfectants were grown under the same conditions as the control, except that G418 was added to the culture medium. miRNA microarray hybridization 2.5 μg of total RNA obtained from MDA-MB-231/vector, MDA-MB-231/E1A and MDA-MB-231/E1A/shDicer cells were labeled and hybridized on miRNA microarrays (using the Human miRNA OneArray® v2 (Phalanx Biotech Group, San Diego, CA, USA). ..

    Multiple Displacement Amplification:

    Article Title: Dicer Elicits Paclitaxel Chemosensitization and Suppresses Cancer Stemness in Breast Cancer by Repressing AXL
    Article Snippet: .. The transfectants were grown under the same conditions as the control, except that G418 was added to the culture medium. miRNA microarray hybridization 2.5 μg of total RNA obtained from MDA-MB-231/vector, MDA-MB-231/E1A and MDA-MB-231/E1A/shDicer cells were labeled and hybridized on miRNA microarrays (using the Human miRNA OneArray® v2 (Phalanx Biotech Group, San Diego, CA, USA). ..

    Labeling:

    Article Title: Dicer Elicits Paclitaxel Chemosensitization and Suppresses Cancer Stemness in Breast Cancer by Repressing AXL
    Article Snippet: .. The transfectants were grown under the same conditions as the control, except that G418 was added to the culture medium. miRNA microarray hybridization 2.5 μg of total RNA obtained from MDA-MB-231/vector, MDA-MB-231/E1A and MDA-MB-231/E1A/shDicer cells were labeled and hybridized on miRNA microarrays (using the Human miRNA OneArray® v2 (Phalanx Biotech Group, San Diego, CA, USA). ..



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    (A)Volcano plot of <t>miRNA</t> <t>expression</t> in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.
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    Image Search Results


    (A)Volcano plot of miRNA expression in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.

    Journal: Circulation. Heart failure

    Article Title: Differential microRNA-21 and microRNA-221 upregulation in the biventricular failing heart reveals distinct stress responses of right versus left ventricular fibroblasts

    doi: 10.1161/CIRCHEARTFAILURE.119.006426

    Figure Lengend Snippet: (A)Volcano plot of miRNA expression in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.

    Article Snippet: miRNA microarray data was analyzed for differential miRNA expression between pre-specified groups (RV-HF vs. RV-Ctrl, LV-HF vs. LV-Ctrl, and RV-HF vs. LV-HF) by two-tailed Student’s t-test using GraphPad Software (Prism 7.0).

    Techniques: Expressing, Labeling, Quantitative RT-PCR, Inhibition