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nikon ti2e microscope  (Nikon)


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    Structured Review

    Nikon nikon ti2e microscope
    Nikon Ti2e Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 63936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microscope+objective/Objectives/pmc12907503-295-33-33
    Average 99 stars, based on 63936 article reviews
    nikon ti2e microscope - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Live Cell Imaging:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Microscopy:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Article Title: Phasic metabolic remodeling coordinates cardiac myofibroblast differentiation and function.
    Article Snippet: The coverslips were washed 3× with DPBS, they were then mounted onto glass slides using Prolong Glass Antifade Mountant (Invitrogen P36982) and allowed to cure for 1 d at room temperature in the dark. .. Cells were imaged using Nikon A1 laser scanning confocal microscope with a Nikon Plan Apo 60×1.4 oil immersion objective. .. Signals from mitochondria, the F-actin cytoskeleton, and nuclei were excited with 561, 488, and 405 nm lasers, respectively, and emissions were collected using 595/50, 525/50, and 450/50 nm band-pass filters, respectively.

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy
    Article Snippet: After three additional washes with PBS, the samples were mounted with Fluoromount-G (Life Technologies, 00-4958-02). .. Confocal images were acquired using a Nikon A1R confocal microscope equipped with a Nikon Plan Fluor HN2 60× oil immersion objective. ..

    Article Title: Mitofusin Activation Delays Aging in Caenorhabditis elegans .
    Article Snippet: .. Confocal images were obtained on a Nikon Eclipse Ti2 confocal microscope using the Nikon CGI Plan Apo VC 60x A/1.20 WI water immersion objective (Nikon Corporation, Tokyo, Japan) [32]. ..

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
    Article Snippet: .. Confocal images of the fluorescent samples were acquired via ×20 (NA 0.75), ×40 (NA 1.30), or ×60 (NA 1.40) objectives of a Nikon A2 confocal microscope. ..

    Fluorescence:

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Electron Microscopy:

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Immunohistochemical staining:

    Article Title: Spatial Remodeling of the Immune Microenvironment During Laryngeal Carcinogenesis Reveals Prognostic Immune Patterns in Laryngeal Squamous Cell Carcinoma.
    Article Snippet: .. Following immunohistochemical staining, all TMA slides were digitized using a PreciPoint Fritz Histo Scanner (PreciPoint GmbH, Garching, Germany) equipped with a Nikon CFI Plan Apochromat 20× objective (Nikon Instruments Inc., Tokyo, Japan) to enable standardized digital pathological evaluation. .. Immunohistochemical evaluation was performed according to the recommendations of the International Immuno-Oncology Biomarker Working Group for the assessment of tumor-infiltrating lymphocytes (TILs) in solid tumors [32], with minor adaptations for TMA-based analysis.

    Staining:

    Article Title: Spatial Remodeling of the Immune Microenvironment During Laryngeal Carcinogenesis Reveals Prognostic Immune Patterns in Laryngeal Squamous Cell Carcinoma.
    Article Snippet: .. Following immunohistochemical staining, all TMA slides were digitized using a PreciPoint Fritz Histo Scanner (PreciPoint GmbH, Garching, Germany) equipped with a Nikon CFI Plan Apochromat 20× objective (Nikon Instruments Inc., Tokyo, Japan) to enable standardized digital pathological evaluation. .. Immunohistochemical evaluation was performed according to the recommendations of the International Immuno-Oncology Biomarker Working Group for the assessment of tumor-infiltrating lymphocytes (TILs) in solid tumors [32], with minor adaptations for TMA-based analysis.

    Confocal:

    Article Title: Phasic metabolic remodeling coordinates cardiac myofibroblast differentiation and function.
    Article Snippet: The coverslips were washed 3× with DPBS, they were then mounted onto glass slides using Prolong Glass Antifade Mountant (Invitrogen P36982) and allowed to cure for 1 d at room temperature in the dark. .. Cells were imaged using Nikon A1 laser scanning confocal microscope with a Nikon Plan Apo 60×1.4 oil immersion objective. .. Signals from mitochondria, the F-actin cytoskeleton, and nuclei were excited with 561, 488, and 405 nm lasers, respectively, and emissions were collected using 595/50, 525/50, and 450/50 nm band-pass filters, respectively.

    Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy
    Article Snippet: After three additional washes with PBS, the samples were mounted with Fluoromount-G (Life Technologies, 00-4958-02). .. Confocal images were acquired using a Nikon A1R confocal microscope equipped with a Nikon Plan Fluor HN2 60× oil immersion objective. ..

    Article Title: Mitofusin Activation Delays Aging in Caenorhabditis elegans .
    Article Snippet: .. Confocal images were obtained on a Nikon Eclipse Ti2 confocal microscope using the Nikon CGI Plan Apo VC 60x A/1.20 WI water immersion objective (Nikon Corporation, Tokyo, Japan) [32]. ..

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
    Article Snippet: .. Confocal images of the fluorescent samples were acquired via ×20 (NA 0.75), ×40 (NA 1.30), or ×60 (NA 1.40) objectives of a Nikon A2 confocal microscope. ..

    Single Cell:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Clone Assay:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Process/Product Development:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..



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    In vitro properties of Im@NOnanoPL nanovaccine. (A) Flow cytometric analysis of DC2.4 cells co‐incubated with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5 after different durations, respectively. (B) Percentage of Cy5 + DC2.4 cells after incubation with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5. (C) Confocal microscopic images of DC2.4 cells after incubating with Im@NOnanoPL‐Cy5 for 2 h and 6 h. The lysosomes stained with Lysotracker Green (green) and amide‐coupled Cy5 labeling for stable cell membrane vesicles surface modification, Scale bar: 10 µm. (D) Evaluation of MHC‐I levels in BMDCs after 12 and 24 h incubation with Im@NOnanoPL and other formulations (NOC‐18: 100 µM), as detected by flow cytometry ( n = 4). (I: PBS, II: m@nanoPL, III: Im@nanoPL, IV: m@NOnanoPL, V: Im@NOnanoPL). (E) Western blot analysis of cGAS‐STING pathway‐related proteins expression in BMDCs treated by Im@NOnanoPL and other formulations (NOC‐18: 100 µM) in 12 h (G1: PBS, G2: m@nanoPL, G3: Im@nanoPL, G4: m@NOnanoPL, G5: Im@NOnanoPL). (F) Experimental design for T cell activation and cytokine release analysis. BMDCs were treated with different formulations for 12 h, followed by co‐culture with CD3 + T cells at <t>a</t> <t>1:10</t> ratio (BMDCs: T cells). The CD3 + T cells were isolated from splenocytes of MC38 tumor‐bearing mice. (G) Percentages of CD69 + or CD25 + in CD3 + CD8 + T cells after 24 h co‐culture, as detected using flow cytometry ( n = 4). (H) Cytokines released by T cells and DCs from different groups after 24 h co‐culture, including IL‐6, IL‐1β, TNF‐α, IFN‐β. Results are presented as mean ± SD ( n = 4). Statistical analysis was performed using the one‐way ANOVA with Tukey's multiple comparisons test.
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    Image Search Results


    In vitro properties of Im@NOnanoPL nanovaccine. (A) Flow cytometric analysis of DC2.4 cells co‐incubated with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5 after different durations, respectively. (B) Percentage of Cy5 + DC2.4 cells after incubation with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5. (C) Confocal microscopic images of DC2.4 cells after incubating with Im@NOnanoPL‐Cy5 for 2 h and 6 h. The lysosomes stained with Lysotracker Green (green) and amide‐coupled Cy5 labeling for stable cell membrane vesicles surface modification, Scale bar: 10 µm. (D) Evaluation of MHC‐I levels in BMDCs after 12 and 24 h incubation with Im@NOnanoPL and other formulations (NOC‐18: 100 µM), as detected by flow cytometry ( n = 4). (I: PBS, II: m@nanoPL, III: Im@nanoPL, IV: m@NOnanoPL, V: Im@NOnanoPL). (E) Western blot analysis of cGAS‐STING pathway‐related proteins expression in BMDCs treated by Im@NOnanoPL and other formulations (NOC‐18: 100 µM) in 12 h (G1: PBS, G2: m@nanoPL, G3: Im@nanoPL, G4: m@NOnanoPL, G5: Im@NOnanoPL). (F) Experimental design for T cell activation and cytokine release analysis. BMDCs were treated with different formulations for 12 h, followed by co‐culture with CD3 + T cells at a 1:10 ratio (BMDCs: T cells). The CD3 + T cells were isolated from splenocytes of MC38 tumor‐bearing mice. (G) Percentages of CD69 + or CD25 + in CD3 + CD8 + T cells after 24 h co‐culture, as detected using flow cytometry ( n = 4). (H) Cytokines released by T cells and DCs from different groups after 24 h co‐culture, including IL‐6, IL‐1β, TNF‐α, IFN‐β. Results are presented as mean ± SD ( n = 4). Statistical analysis was performed using the one‐way ANOVA with Tukey's multiple comparisons test.

    Journal: Exploration

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity

    doi: 10.1002/exp2.70238

    Figure Lengend Snippet: In vitro properties of Im@NOnanoPL nanovaccine. (A) Flow cytometric analysis of DC2.4 cells co‐incubated with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5 after different durations, respectively. (B) Percentage of Cy5 + DC2.4 cells after incubation with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5. (C) Confocal microscopic images of DC2.4 cells after incubating with Im@NOnanoPL‐Cy5 for 2 h and 6 h. The lysosomes stained with Lysotracker Green (green) and amide‐coupled Cy5 labeling for stable cell membrane vesicles surface modification, Scale bar: 10 µm. (D) Evaluation of MHC‐I levels in BMDCs after 12 and 24 h incubation with Im@NOnanoPL and other formulations (NOC‐18: 100 µM), as detected by flow cytometry ( n = 4). (I: PBS, II: m@nanoPL, III: Im@nanoPL, IV: m@NOnanoPL, V: Im@NOnanoPL). (E) Western blot analysis of cGAS‐STING pathway‐related proteins expression in BMDCs treated by Im@NOnanoPL and other formulations (NOC‐18: 100 µM) in 12 h (G1: PBS, G2: m@nanoPL, G3: Im@nanoPL, G4: m@NOnanoPL, G5: Im@NOnanoPL). (F) Experimental design for T cell activation and cytokine release analysis. BMDCs were treated with different formulations for 12 h, followed by co‐culture with CD3 + T cells at a 1:10 ratio (BMDCs: T cells). The CD3 + T cells were isolated from splenocytes of MC38 tumor‐bearing mice. (G) Percentages of CD69 + or CD25 + in CD3 + CD8 + T cells after 24 h co‐culture, as detected using flow cytometry ( n = 4). (H) Cytokines released by T cells and DCs from different groups after 24 h co‐culture, including IL‐6, IL‐1β, TNF‐α, IFN‐β. Results are presented as mean ± SD ( n = 4). Statistical analysis was performed using the one‐way ANOVA with Tukey's multiple comparisons test.

    Article Snippet: Imaging was performed on a Nikon A1R HD25 confocal microscope using a 60× oil objective.

    Techniques: In Vitro, Analysis, Incubation, Confocal, Staining, Labeling, Stable Transfection, Membrane, Modification, Flow Cytometry, Western Blot, Expressing, Activation Assay, Co-culture Assay, Isolation