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i control microplate reader  (Tecan Systems)


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    Structured Review

    Tecan Systems i control microplate reader
    DDRGK1 regulates NRF2 in a UFMylation‐independent pathway. A) Interaction between NRF2 and UFM1. The NRF2‐HA and ufm1‐HA plasmids were transfected into HEK293T cells. The interaction between NRF2 and ufm1 was detected by co‐immunoprecipitation with HA antibody followed by western blot with NRF2 and ufm1 antibody. B) Interaction between NRF2 and DDRGK1. The NRF2‐HA and DDRGK1‐Flag plasmids were transfected into HEK293T cells. The interaction between NRF2 and DDRGK1 was detected by co‐immunoprecipitation using anti‐HA immunomagnetic beads and anti‐Flag immunomagnetic beads. C) NRF2 levels of heart, lung, kidney, and cartilage in WT and K268R mouse detected by western blot. D) Re‐express of WT‐DDRGK1 and K267R‐DDRGK1 in DDRGK1 deficient cells rescued level of NRF2. E) Mitochondrial metabolism analysis. Cells were seeded in an XF <t>microplate</t> at an optimal density and cultured for 24 h. The cellular oxygen consumption rate (OCR) was measured using a Seahorse XF Analyzer. F) ROS level measurement. The controlled, DDRGK1‐knockout cells, and WT‐DDRGK1/K267R‐DDRGK1 re‐expressed cells were subjected to H 2 O 2 , then loaded with a DHE probe for 30 min and detected by flow cemetery. (MFI, mean fluorescence intensity; Three samples for each statistical analysis **** p < 0.0001).
    I Control Microplate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 97/100, based on 1060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microprocessor+controlled+microplate+reader/Microplate+Carrier%2C+4+Position%2C+Landscape%2C+Standard+Height/pmc10190621-244-27-30
    Average 97 stars, based on 1060 article reviews
    i control microplate reader - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1‐Mediated NRF2 Ubiquitination"

    Article Title: DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1‐Mediated NRF2 Ubiquitination

    Journal: Advanced Science

    doi: 10.1002/advs.202204438

    DDRGK1 regulates NRF2 in a UFMylation‐independent pathway. A) Interaction between NRF2 and UFM1. The NRF2‐HA and ufm1‐HA plasmids were transfected into HEK293T cells. The interaction between NRF2 and ufm1 was detected by co‐immunoprecipitation with HA antibody followed by western blot with NRF2 and ufm1 antibody. B) Interaction between NRF2 and DDRGK1. The NRF2‐HA and DDRGK1‐Flag plasmids were transfected into HEK293T cells. The interaction between NRF2 and DDRGK1 was detected by co‐immunoprecipitation using anti‐HA immunomagnetic beads and anti‐Flag immunomagnetic beads. C) NRF2 levels of heart, lung, kidney, and cartilage in WT and K268R mouse detected by western blot. D) Re‐express of WT‐DDRGK1 and K267R‐DDRGK1 in DDRGK1 deficient cells rescued level of NRF2. E) Mitochondrial metabolism analysis. Cells were seeded in an XF microplate at an optimal density and cultured for 24 h. The cellular oxygen consumption rate (OCR) was measured using a Seahorse XF Analyzer. F) ROS level measurement. The controlled, DDRGK1‐knockout cells, and WT‐DDRGK1/K267R‐DDRGK1 re‐expressed cells were subjected to H 2 O 2 , then loaded with a DHE probe for 30 min and detected by flow cemetery. (MFI, mean fluorescence intensity; Three samples for each statistical analysis **** p < 0.0001).
    Figure Legend Snippet: DDRGK1 regulates NRF2 in a UFMylation‐independent pathway. A) Interaction between NRF2 and UFM1. The NRF2‐HA and ufm1‐HA plasmids were transfected into HEK293T cells. The interaction between NRF2 and ufm1 was detected by co‐immunoprecipitation with HA antibody followed by western blot with NRF2 and ufm1 antibody. B) Interaction between NRF2 and DDRGK1. The NRF2‐HA and DDRGK1‐Flag plasmids were transfected into HEK293T cells. The interaction between NRF2 and DDRGK1 was detected by co‐immunoprecipitation using anti‐HA immunomagnetic beads and anti‐Flag immunomagnetic beads. C) NRF2 levels of heart, lung, kidney, and cartilage in WT and K268R mouse detected by western blot. D) Re‐express of WT‐DDRGK1 and K267R‐DDRGK1 in DDRGK1 deficient cells rescued level of NRF2. E) Mitochondrial metabolism analysis. Cells were seeded in an XF microplate at an optimal density and cultured for 24 h. The cellular oxygen consumption rate (OCR) was measured using a Seahorse XF Analyzer. F) ROS level measurement. The controlled, DDRGK1‐knockout cells, and WT‐DDRGK1/K267R‐DDRGK1 re‐expressed cells were subjected to H 2 O 2 , then loaded with a DHE probe for 30 min and detected by flow cemetery. (MFI, mean fluorescence intensity; Three samples for each statistical analysis **** p < 0.0001).

    Techniques Used: Transfection, Immunoprecipitation, Western Blot, Cell Culture, Knock-Out, Fluorescence

    Related Articles

    Fluorescence:

    Article Title: Identification of antimicrobial peptides from the Ambystoma mexicanum displaying antibacterial and antitumor activity.
    Article Snippet: .. After 60 minutes, fluorescence was measured using the Tecan microplate reader excitation wavelength range of 485 ± 20nm and an emission wavelength range of 530 ± 25nm. (Tecan Group, Schweiz). ..

    Article Title: Identification of antimicrobial peptides from the Ambystoma mexicanum displaying antibacterial and antitumor activity
    Article Snippet: .. After 60 minutes, fluorescence was measured using the Tecan microplate reader excitation wavelength range of 485 ± 20nm and an emission wavelength range of 530 ± 25nm. (Tecan Group, Schweiz). ..

    Incubation:

    Article Title: L1CAM mimetic compound duloxetine improves cognitive impairment in 5xFAD mice and protects Aβ1-42-damaged HT22 cells.
    Article Snippet: Background: Synapse loss and damage are underlying causes of Alzheimer’s disease.. Duloxetine has been identified as a mimetic of neural adhesion molecule L1CAM, a neuronal synapse component, suggesting duloxetine could be therapeutic for Alzheimer’s disease.. Methods: Cognitive function in 5xFAD mice was evaluated by open field, novel object recognition, and Morris water maze tests.



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