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spectramax m2e plate reader  (Danaher Inc)


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    Structured Review

    Danaher Inc spectramax m2e plate reader
    Cytotoxicity of pooled human serum against RBCs. There was no cytotoxicity of pooled human serum against TKO pRBCs or human blood type O RBCs. When the concentration of human serum was 25% or less, the cytotoxicity against GTKO pRBCs was significantly lower than that against WT pRBCs (p < 0.01). See “ ” section (Antibody-dependent complement-mediated hemolytic assay). Briefly, RBCs (800 × 10 6 /ml in 75 µl) were isolated and placed in 5 ml round-bottom tubes. Titrated non-heat-inactivated serum (i.e. with complement activity; 450 µl) with PBS and 5% sorbitol (375 µl) instead of IVIg was added to each tube (total 900 µl) and incubated at 37 °C for 150min . Control samples consisted of PBS (instead of the RBC solution) + 450 µl of titrated non-heat-inactivated serum with PBS + 5% sorbitol. After centrifugation at 910 g for 5 min, the supernatant was collected, and each 300 µl was transferred into 96-well plates. The released hemoglobin was measured at an optical density (OD) of 541 nm using a <t>SpectraMax</t> <t>M2e</t> plate reader (Molecular Devices Corp). Data were obtained in duplicate. Results are expressed as mean + /− SD. The dotted line represents cut-off value (7%). (**p < 0.01).
    Spectramax M2e Plate Reader, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microprocessor+controlled+microplate+reader/SpectraMax+M2E+Series+Microplate+Readers/pmc07367287-67-15-19
    Average 96 stars, based on 1949 article reviews
    spectramax m2e plate reader - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Effect of intravenous immunoglobulin (IVIg) on primate complement-dependent cytotoxicity of genetically engineered pig cells: relevance to clinical xenotransplantation"

    Article Title: Effect of intravenous immunoglobulin (IVIg) on primate complement-dependent cytotoxicity of genetically engineered pig cells: relevance to clinical xenotransplantation

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-68505-1

    Cytotoxicity of pooled human serum against RBCs. There was no cytotoxicity of pooled human serum against TKO pRBCs or human blood type O RBCs. When the concentration of human serum was 25% or less, the cytotoxicity against GTKO pRBCs was significantly lower than that against WT pRBCs (p < 0.01). See “ ” section (Antibody-dependent complement-mediated hemolytic assay). Briefly, RBCs (800 × 10 6 /ml in 75 µl) were isolated and placed in 5 ml round-bottom tubes. Titrated non-heat-inactivated serum (i.e. with complement activity; 450 µl) with PBS and 5% sorbitol (375 µl) instead of IVIg was added to each tube (total 900 µl) and incubated at 37 °C for 150min . Control samples consisted of PBS (instead of the RBC solution) + 450 µl of titrated non-heat-inactivated serum with PBS + 5% sorbitol. After centrifugation at 910 g for 5 min, the supernatant was collected, and each 300 µl was transferred into 96-well plates. The released hemoglobin was measured at an optical density (OD) of 541 nm using a SpectraMax M2e plate reader (Molecular Devices Corp). Data were obtained in duplicate. Results are expressed as mean + /− SD. The dotted line represents cut-off value (7%). (**p < 0.01).
    Figure Legend Snippet: Cytotoxicity of pooled human serum against RBCs. There was no cytotoxicity of pooled human serum against TKO pRBCs or human blood type O RBCs. When the concentration of human serum was 25% or less, the cytotoxicity against GTKO pRBCs was significantly lower than that against WT pRBCs (p < 0.01). See “ ” section (Antibody-dependent complement-mediated hemolytic assay). Briefly, RBCs (800 × 10 6 /ml in 75 µl) were isolated and placed in 5 ml round-bottom tubes. Titrated non-heat-inactivated serum (i.e. with complement activity; 450 µl) with PBS and 5% sorbitol (375 µl) instead of IVIg was added to each tube (total 900 µl) and incubated at 37 °C for 150min . Control samples consisted of PBS (instead of the RBC solution) + 450 µl of titrated non-heat-inactivated serum with PBS + 5% sorbitol. After centrifugation at 910 g for 5 min, the supernatant was collected, and each 300 µl was transferred into 96-well plates. The released hemoglobin was measured at an optical density (OD) of 541 nm using a SpectraMax M2e plate reader (Molecular Devices Corp). Data were obtained in duplicate. Results are expressed as mean + /− SD. The dotted line represents cut-off value (7%). (**p < 0.01).

    Techniques Used: Concentration Assay, Hemolytic Assay, Isolation, Activity Assay, Incubation, Control, Centrifugation



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