Journal: iScience

Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function

doi: 10.1016/j.isci.2019.10.061

Figure Lengend Snippet: Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide microarray in A. Data are represented as mean ± SEM.

Article Snippet: Amine(-NH 2 )-linked heparan sulfate glycan compounds (Glycan Therapeutics) were immobilized on NHS-activated surface-coated slides (Nexterion Slide-H, Applied Microarrays) using a robotic microarray printer (Microgrid II, Digilab) that was equipped with StealthSMP4B microarray pins (Telechem) to couple heparan sulfate glycan compounds by covalent binding via (-NH 2 ) reactive chemistry.

Techniques: Glycoproteomics, Binding Assay, Fluorescence, Molecular Weight, Microarray

Journal:

Article Title: DYNAMICS OF 17α-ETHYNYLESTRADIOL EXPOSURE IN RAINBOW TROUT ( ONCORHYNCHUS MYKISS ): ABSORPTION, TISSUE DISTRIBUTION, AND HEPATIC GENE EXPRESSION PATTERN

doi:

Figure Lengend Snippet: Hepatic gene expression in sexually mature male rainbow trout (Onchorynchus mykiss) measured using a low-density cDNA microarray. Three trout were individually exposed for 7 d to a measured 17α-ethynylestradiol (EE2) concentration of 75 ng/L. A total of 32 cDNA sequences were on the microarray and are listed below the figure. Results are expressed as the mean fold-change relative to control trout that received only the methanol carrier.

Article Snippet: Targets were concentrated using a heated vacuum centrifuge to a concentration of at least 400 ng/μl (range, 398–600 ng/μl) and then spotted onto aminosilane-coated microarray slides using a Microgrid robotic microarray printer (Apogent Discoveries, Hudson, NH, USA).

Techniques: Expressing, Microarray, Concentration Assay

Journal:

Article Title: DYNAMICS OF 17α-ETHYNYLESTRADIOL EXPOSURE IN RAINBOW TROUT ( ONCORHYNCHUS MYKISS ): ABSORPTION, TISSUE DISTRIBUTION, AND HEPATIC GENE EXPRESSION PATTERN

doi:

Figure Lengend Snippet: Summary of gene expression changes in trout liver using the Genomic Research on Atlantic Salmon Project (GRASP) high-density salmonid cDNA microarray a

Article Snippet: Targets were concentrated using a heated vacuum centrifuge to a concentration of at least 400 ng/μl (range, 398–600 ng/μl) and then spotted onto aminosilane-coated microarray slides using a Microgrid robotic microarray printer (Apogent Discoveries, Hudson, NH, USA).

Techniques: Expressing, Microarray

Journal:

Article Title: DYNAMICS OF 17α-ETHYNYLESTRADIOL EXPOSURE IN RAINBOW TROUT ( ONCORHYNCHUS MYKISS ): ABSORPTION, TISSUE DISTRIBUTION, AND HEPATIC GENE EXPRESSION PATTERN

doi:

Figure Lengend Snippet: Comparison between the high density microarray data and quantitative polymerase chain reaction (qPCR) data a

Article Snippet: Targets were concentrated using a heated vacuum centrifuge to a concentration of at least 400 ng/μl (range, 398–600 ng/μl) and then spotted onto aminosilane-coated microarray slides using a Microgrid robotic microarray printer (Apogent Discoveries, Hudson, NH, USA).

Techniques: Microarray, Real-time Polymerase Chain Reaction