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robotic microarray printer microgrid ii  (Digilab Inc)

 
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    Structured Review

    Digilab Inc robotic microarray printer microgrid ii
    Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide <t>microarray</t> in A. Data are represented as mean ± SEM.
    Robotic Microarray Printer Microgrid Ii, supplied by Digilab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microgrid+robotic+microarray+printer/microarraying+robot+microgrid+610/pmc06928319-64-21-26
    Average 90 stars, based on 1 article reviews
    robotic microarray printer microgrid ii - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function"

    Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function

    Journal: iScience

    doi: 10.1016/j.isci.2019.10.061

    Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide microarray in A. Data are represented as mean ± SEM.
    Figure Legend Snippet: Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide microarray in A. Data are represented as mean ± SEM.

    Techniques Used: Glycoproteomics, Binding Assay, Fluorescence, Molecular Weight, Microarray

    Related Articles

    Microarray:

    Article Title: The role of indoleamine 2,3-dioxygenase-aryl hydrocarbon receptor pathway in the TLR4-induced tolerogenic phenotype in human DCs.
    Article Snippet: .. After denaturation, samples were spotted onto nitrocellulose-coated glass slides (Grace Bio-labs) with a microarray robot (MicroGrid 610, Digilab). .. Then, slides were incubated overnight in blocking solution (0.2% I-Block (Thermo Fisher Scientific), Tween-20 (0.1%) in PBS (Sigma-Aldrich) at 4 °C.

    Article Title: Clinical utility of reverse phase protein array for molecular classification of breast cancer.
    Article Snippet: Reverse Phase Protein Array (RPPA) represents a sensitive and high-throughput technique allowing simultaneous quantitation of protein expression levels in biological samples.. This study aimed to confirm the ability of RPPA to classify archival formalin-fixed paraffin-embedded (FFPE) breast cancer tissues into molecular classes used in the Nottingham prognostic index plus (NPI?) determined by immunohistochemistry (IHC).. Proteins were extracted from FFPE breast cancer tissues using three extraction protocols: the Q-proteome FFPE Tissue Kit (Qiagen, Hilden, Germany) and two in-house methods using Laemmli buffer with either incubation for 20 min or 2 h at 105 C. Two preparation methods, full-face sections and macrodissection, were used to assess the yield and quality of protein extracts.

    Article Title: The mannose receptor negatively modulates the Toll-like receptor 4-aryl hydrocarbon receptor-indoleamine 2,3-dioxygenase axis in dendritic cells affecting T helper cell polarization.
    Article Snippet: .. After denaturation, samples were spotted onto nitrocellulose-coated glass slides (Grace Bio-Labs, Bend, Ore) with a microarray robot (MicroGrid 610; Digilab, Marlborough, Mass). .. Then slides were incubated overnight in blocking solution (0.2% IBlock [Thermo Fisher Scientific] and 0.1% Tween-20 in PBS [SigmaAldrich]) at 48C.

    Article Title: A Pro-Inflammatory Signature Constitutively Activated in Monogenic Autoinflammatory Diseases
    Article Snippet: .. Briefly, samples were spotted in duplicates onto a nitrocellulose-coated glass slide (Grace Bio-labs, Bend, OR, USA) using a microarray robot (MicroGrid 610, Digilab, Marlborough, MA, USA). .. The printed slides were blocked and incubated with specific primary antibodies listed in (Cell Signalling) overnight at 4 °C, with shaking.

    Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function
    Article Snippet: .. Amine(-NH 2 )-linked heparan sulfate glycan compounds (Glycan Therapeutics) were immobilized on NHS-activated surface-coated slides (Nexterion Slide-H, Applied Microarrays) using a robotic microarray printer (Microgrid II, Digilab) that was equipped with StealthSMP4B microarray pins (Telechem) to couple heparan sulfate glycan compounds by covalent binding via (-NH 2 ) reactive chemistry. ..

    Article Title: The role of indoleamine 2,3-dioxygenase-aryl hydrocarbon receptor pathway in the TLR4-induced tolerogenic phenotype in human DCs
    Article Snippet: .. After denaturation, samples were spotted onto nitrocellulose-coated glass slides (Grace Bio-labs) with a microarray robot (MicroGrid 610, Digilab). .. Then, slides were incubated overnight in blocking solution (0.2% I-Block (Thermo Fisher Scientific), Tween-20 (0.1%) in PBS (Sigma-Aldrich) at 4 °C.

    other:

    Article Title: New and Improved Techniques for the Study of Pathogenic Fungi.
    Article Snippet: Fungal pathogens pose serious threats to human, plant, and ecosystem health.. Improved diagnostics and antifungal strategies are therefore urgently required.. Here, we review recent developments in online bioinformatic tools and associated interactive data archives, which enable sophisticated comparative genomics and functional analysis of fungal pathogens in silico.

    Glycoproteomics:

    Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function
    Article Snippet: .. Amine(-NH 2 )-linked heparan sulfate glycan compounds (Glycan Therapeutics) were immobilized on NHS-activated surface-coated slides (Nexterion Slide-H, Applied Microarrays) using a robotic microarray printer (Microgrid II, Digilab) that was equipped with StealthSMP4B microarray pins (Telechem) to couple heparan sulfate glycan compounds by covalent binding via (-NH 2 ) reactive chemistry. ..

    Binding Assay:

    Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function
    Article Snippet: .. Amine(-NH 2 )-linked heparan sulfate glycan compounds (Glycan Therapeutics) were immobilized on NHS-activated surface-coated slides (Nexterion Slide-H, Applied Microarrays) using a robotic microarray printer (Microgrid II, Digilab) that was equipped with StealthSMP4B microarray pins (Telechem) to couple heparan sulfate glycan compounds by covalent binding via (-NH 2 ) reactive chemistry. ..



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    Digilab Inc robotic microarray printer microgrid ii
    Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide <t>microarray</t> in A. Data are represented as mean ± SEM.
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    microgrid robotic microarray printer  (Thermo Fisher)
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    Hepatic gene expression in sexually mature male rainbow trout (Onchorynchus mykiss) measured using a low-density cDNA <t>microarray.</t> Three trout were individually exposed for 7 d to a measured 17α-ethynylestradiol (EE2) concentration of 75 ng/L. A total of 32 cDNA sequences were on the microarray and are listed below the figure. Results are expressed as the mean fold-change relative to control trout that received only the methanol carrier.
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    Image Search Results


    Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide microarray in A. Data are represented as mean ± SEM.

    Journal: iScience

    Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function

    doi: 10.1016/j.isci.2019.10.061

    Figure Lengend Snippet: Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide microarray in A. Data are represented as mean ± SEM.

    Article Snippet: Amine(-NH 2 )-linked heparan sulfate glycan compounds (Glycan Therapeutics) were immobilized on NHS-activated surface-coated slides (Nexterion Slide-H, Applied Microarrays) using a robotic microarray printer (Microgrid II, Digilab) that was equipped with StealthSMP4B microarray pins (Telechem) to couple heparan sulfate glycan compounds by covalent binding via (-NH 2 ) reactive chemistry.

    Techniques: Glycoproteomics, Binding Assay, Fluorescence, Molecular Weight, Microarray

    Hepatic gene expression in sexually mature male rainbow trout (Onchorynchus mykiss) measured using a low-density cDNA microarray. Three trout were individually exposed for 7 d to a measured 17α-ethynylestradiol (EE2) concentration of 75 ng/L. A total of 32 cDNA sequences were on the microarray and are listed below the figure. Results are expressed as the mean fold-change relative to control trout that received only the methanol carrier.

    Journal:

    Article Title: DYNAMICS OF 17α-ETHYNYLESTRADIOL EXPOSURE IN RAINBOW TROUT ( ONCORHYNCHUS MYKISS ): ABSORPTION, TISSUE DISTRIBUTION, AND HEPATIC GENE EXPRESSION PATTERN

    doi:

    Figure Lengend Snippet: Hepatic gene expression in sexually mature male rainbow trout (Onchorynchus mykiss) measured using a low-density cDNA microarray. Three trout were individually exposed for 7 d to a measured 17α-ethynylestradiol (EE2) concentration of 75 ng/L. A total of 32 cDNA sequences were on the microarray and are listed below the figure. Results are expressed as the mean fold-change relative to control trout that received only the methanol carrier.

    Article Snippet: Targets were concentrated using a heated vacuum centrifuge to a concentration of at least 400 ng/μl (range, 398–600 ng/μl) and then spotted onto aminosilane-coated microarray slides using a Microgrid robotic microarray printer (Apogent Discoveries, Hudson, NH, USA).

    Techniques: Expressing, Microarray, Concentration Assay

    Summary of gene expression changes in trout liver using the Genomic Research on Atlantic Salmon Project (GRASP) high-density salmonid cDNA microarray a

    Journal:

    Article Title: DYNAMICS OF 17α-ETHYNYLESTRADIOL EXPOSURE IN RAINBOW TROUT ( ONCORHYNCHUS MYKISS ): ABSORPTION, TISSUE DISTRIBUTION, AND HEPATIC GENE EXPRESSION PATTERN

    doi:

    Figure Lengend Snippet: Summary of gene expression changes in trout liver using the Genomic Research on Atlantic Salmon Project (GRASP) high-density salmonid cDNA microarray a

    Article Snippet: Targets were concentrated using a heated vacuum centrifuge to a concentration of at least 400 ng/μl (range, 398–600 ng/μl) and then spotted onto aminosilane-coated microarray slides using a Microgrid robotic microarray printer (Apogent Discoveries, Hudson, NH, USA).

    Techniques: Expressing, Microarray

    Comparison between the high density microarray data and quantitative polymerase chain reaction (qPCR) data a

    Journal:

    Article Title: DYNAMICS OF 17α-ETHYNYLESTRADIOL EXPOSURE IN RAINBOW TROUT ( ONCORHYNCHUS MYKISS ): ABSORPTION, TISSUE DISTRIBUTION, AND HEPATIC GENE EXPRESSION PATTERN

    doi:

    Figure Lengend Snippet: Comparison between the high density microarray data and quantitative polymerase chain reaction (qPCR) data a

    Article Snippet: Targets were concentrated using a heated vacuum centrifuge to a concentration of at least 400 ng/μl (range, 398–600 ng/μl) and then spotted onto aminosilane-coated microarray slides using a Microgrid robotic microarray printer (Apogent Discoveries, Hudson, NH, USA).

    Techniques: Microarray, Real-time Polymerase Chain Reaction