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microgrid ii automated microarrayer  (BioRobotics Ltd)

 
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    BioRobotics Ltd microgrid ii automated microarrayer
    Microgrid Ii Automated Microarrayer, supplied by BioRobotics Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microgrid+robotic+microarray+printer/microgrid+ii+arrayer/pmc03145110-50-20-24
    Average 90 stars, based on 1 article reviews
    microgrid ii automated microarrayer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification and characterisation of human apoptosis inducing proteins using cell-based transfection microarrays and expression analysis
    Article Snippet: 1 μg IRAT plasmids, pEGFP-C1 vector (Clontech) and TNFRSF10B, IL17BR, NFIB, CDKN1B, NFIL3 and PTPN11 in the pcDNA-DEST47 vector (Invitrogen) were made up to 30 μl with 0.3% gelatin (Sigma) and transferred into 384-well plates. .. Clones were printed onto poly-lysine slides (Sigma) using a Biorobotics MicroGrid II Microarrayer (Biorobotics, Cambridge, UK) with a 48 pin head (Quill pins 2500, Biorobotics). ..

    Hybridization:

    Article Title: PMC42, a breast progenitor cancer cell line, has normal-like mRNA and microRNA transcriptomes
    Article Snippet: .. Comparative genomic hybridisation (CGH) on bacterial artificial chromosome (BAC) arrays was performed as described [ , ], essentially according to the methods of [ ] using an in-house array [ ] comprising DNA amplified from BACs 10 Mb or less apart across the whole genome spotted in triplicate onto amine-binding slides (CodeLink Activated Slides; Amersham Biosciences, now part of GE Healthcare) using a MicroGrid II arrayer (BioRobotics, Boston, MA, USA). ..

    BAC Assay:

    Article Title: PMC42, a breast progenitor cancer cell line, has normal-like mRNA and microRNA transcriptomes
    Article Snippet: .. Comparative genomic hybridisation (CGH) on bacterial artificial chromosome (BAC) arrays was performed as described [ , ], essentially according to the methods of [ ] using an in-house array [ ] comprising DNA amplified from BACs 10 Mb or less apart across the whole genome spotted in triplicate onto amine-binding slides (CodeLink Activated Slides; Amersham Biosciences, now part of GE Healthcare) using a MicroGrid II arrayer (BioRobotics, Boston, MA, USA). ..

    Amplification:

    Article Title: PMC42, a breast progenitor cancer cell line, has normal-like mRNA and microRNA transcriptomes
    Article Snippet: .. Comparative genomic hybridisation (CGH) on bacterial artificial chromosome (BAC) arrays was performed as described [ , ], essentially according to the methods of [ ] using an in-house array [ ] comprising DNA amplified from BACs 10 Mb or less apart across the whole genome spotted in triplicate onto amine-binding slides (CodeLink Activated Slides; Amersham Biosciences, now part of GE Healthcare) using a MicroGrid II arrayer (BioRobotics, Boston, MA, USA). ..

    other:

    Article Title: Immunoanalytical Detection of Conserved Peptides: Refining the Universe of Biomarker Targets in Planetary Exploration
    Article Snippet: Printing was done by using a three-spot pattern on epoxy activated glass slides (Arrayit) with a MicroGrid II TAS 600 arrayer (BioRobotics).

    Article Title: A pig multi-tissue normalised cDNA library: large-scale sequencing, cluster analysis and 9K micro-array resource generation
    Article Snippet: unpurified PCR products were evaporated, resuspended in 20 μl of distilled water, then transferred to 384-well microplates and spotted ontonylon membranes (Hybond-N+; Amersham Biosciences, Saclay, France), using a Biorobotics MicroGrid-II arrayer (Genomics Solution, Cambridge, U.K.) equipped with a 64-pins Bioroboticsprinthead and 64 Biorobotics 100 μm solid pins.

    Microarray:

    Article Title: Mosquito transcriptome changes and filarial worm resistance in Armigeres subalbatus
    Article Snippet: Oligos were designed using OligoArray v 2.1 [ ], and these 6,143 oligonucleotides were used to construct our Armigeres microarray. .. Ar. subalbatus microarray slides (Corning Ultragaps) were printed using BioRobotics 10 k pins with a BioRobotics Microgrid II arrayer (23°C and 50% humidity). .. Sixty-mer oligonucleotides were re-suspended at 40 μM in Pronto spotting buffer (Promega, Madison, WI).

    Article Title: Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays
    Article Snippet: .. Several DNA microarrays [ , , , , , , , ], glycan microarrays [ , , , , ], peptide microarrays [ , ], and protein microarrays [ , , , , ] are fabricated using commercially available instruments, e.g., Smart Arrayer, OmniGrid contact microarrayer, Gene chip, Bio odyssey, BioRobotics MicroGRID II microarray printer, etc. ..

    Software:

    Article Title: Immunoanalytical Approach for Detecting and Identifying Ancestral Peptide Biomarkers in Early Earth Analogue Environments
    Article Snippet: .. Microarrays containing Ancestral-Protein antibodies (APChip) or ancestral-PEptides (PEChip) were printed in a triplicate (APChip) or duplicate (PEChip) spot pattern on epoxyactivated glass slides (Cel Associates Inc., Pearland, TX, USA) with a MicroGrid II TAS 600 spotting arrayer (BioRobotics, DIGILAB, Inc., Marlborough, MA) using the TAS Application Suite software for run programming and execution. .. Printing solutions contained Protein Printing Buffer (PPB 1X, Sigma-Aldrich) with 0.02% Tween20, BSA and PPB were also printed as controls.

    Glycoproteomics:

    Article Title: Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays
    Article Snippet: .. Several DNA microarrays [ , , , , , , , ], glycan microarrays [ , , , , ], peptide microarrays [ , ], and protein microarrays [ , , , , ] are fabricated using commercially available instruments, e.g., Smart Arrayer, OmniGrid contact microarrayer, Gene chip, Bio odyssey, BioRobotics MicroGRID II microarray printer, etc. ..



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    Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide <t>microarray</t> in A. Data are represented as mean ± SEM.
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    microgrid robotic microarray printer  (Thermo Fisher)
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    Hepatic gene expression in sexually mature male rainbow trout (Onchorynchus mykiss) measured using a low-density cDNA <t>microarray.</t> Three trout were individually exposed for 7 d to a measured 17α-ethynylestradiol (EE2) concentration of 75 ng/L. A total of 32 cDNA sequences were on the microarray and are listed below the figure. Results are expressed as the mean fold-change relative to control trout that received only the methanol carrier.
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    Image Search Results


    Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide microarray in A. Data are represented as mean ± SEM.

    Journal: iScience

    Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function

    doi: 10.1016/j.isci.2019.10.061

    Figure Lengend Snippet: Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide microarray in A. Data are represented as mean ± SEM.

    Article Snippet: Amine(-NH 2 )-linked heparan sulfate glycan compounds (Glycan Therapeutics) were immobilized on NHS-activated surface-coated slides (Nexterion Slide-H, Applied Microarrays) using a robotic microarray printer (Microgrid II, Digilab) that was equipped with StealthSMP4B microarray pins (Telechem) to couple heparan sulfate glycan compounds by covalent binding via (-NH 2 ) reactive chemistry.

    Techniques: Glycoproteomics, Binding Assay, Fluorescence, Molecular Weight, Microarray

    Hepatic gene expression in sexually mature male rainbow trout (Onchorynchus mykiss) measured using a low-density cDNA microarray. Three trout were individually exposed for 7 d to a measured 17α-ethynylestradiol (EE2) concentration of 75 ng/L. A total of 32 cDNA sequences were on the microarray and are listed below the figure. Results are expressed as the mean fold-change relative to control trout that received only the methanol carrier.

    Journal:

    Article Title: DYNAMICS OF 17α-ETHYNYLESTRADIOL EXPOSURE IN RAINBOW TROUT ( ONCORHYNCHUS MYKISS ): ABSORPTION, TISSUE DISTRIBUTION, AND HEPATIC GENE EXPRESSION PATTERN

    doi:

    Figure Lengend Snippet: Hepatic gene expression in sexually mature male rainbow trout (Onchorynchus mykiss) measured using a low-density cDNA microarray. Three trout were individually exposed for 7 d to a measured 17α-ethynylestradiol (EE2) concentration of 75 ng/L. A total of 32 cDNA sequences were on the microarray and are listed below the figure. Results are expressed as the mean fold-change relative to control trout that received only the methanol carrier.

    Article Snippet: Targets were concentrated using a heated vacuum centrifuge to a concentration of at least 400 ng/μl (range, 398–600 ng/μl) and then spotted onto aminosilane-coated microarray slides using a Microgrid robotic microarray printer (Apogent Discoveries, Hudson, NH, USA).

    Techniques: Expressing, Microarray, Concentration Assay

    Summary of gene expression changes in trout liver using the Genomic Research on Atlantic Salmon Project (GRASP) high-density salmonid cDNA microarray a

    Journal:

    Article Title: DYNAMICS OF 17α-ETHYNYLESTRADIOL EXPOSURE IN RAINBOW TROUT ( ONCORHYNCHUS MYKISS ): ABSORPTION, TISSUE DISTRIBUTION, AND HEPATIC GENE EXPRESSION PATTERN

    doi:

    Figure Lengend Snippet: Summary of gene expression changes in trout liver using the Genomic Research on Atlantic Salmon Project (GRASP) high-density salmonid cDNA microarray a

    Article Snippet: Targets were concentrated using a heated vacuum centrifuge to a concentration of at least 400 ng/μl (range, 398–600 ng/μl) and then spotted onto aminosilane-coated microarray slides using a Microgrid robotic microarray printer (Apogent Discoveries, Hudson, NH, USA).

    Techniques: Expressing, Microarray

    Comparison between the high density microarray data and quantitative polymerase chain reaction (qPCR) data a

    Journal:

    Article Title: DYNAMICS OF 17α-ETHYNYLESTRADIOL EXPOSURE IN RAINBOW TROUT ( ONCORHYNCHUS MYKISS ): ABSORPTION, TISSUE DISTRIBUTION, AND HEPATIC GENE EXPRESSION PATTERN

    doi:

    Figure Lengend Snippet: Comparison between the high density microarray data and quantitative polymerase chain reaction (qPCR) data a

    Article Snippet: Targets were concentrated using a heated vacuum centrifuge to a concentration of at least 400 ng/μl (range, 398–600 ng/μl) and then spotted onto aminosilane-coated microarray slides using a Microgrid robotic microarray printer (Apogent Discoveries, Hudson, NH, USA).

    Techniques: Microarray, Real-time Polymerase Chain Reaction