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microneedles platinum heating filament  (World Precision Instruments)


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    Structured Review

    World Precision Instruments microneedles platinum heating filament
    Microneedles Platinum Heating Filament, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microforge/Replacement+Heating+Filament+for+MF200+Microforge%2C+Small+gauge/pmc04117203-169-33-37
    Average 94 stars, based on 6 article reviews
    microneedles platinum heating filament - by Bioz Stars, 2026-10
    94/100 stars

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    other:

    Article Title: Cholesterol activates BK channels by increasing KCNMB1 protein levels in the plasmalemma
    Article Snippet: Patchrecording glass electrodes were fire-polished on a microforge WPI MF-200 (World Precision Instruments) to give resistances of 5–9 MΩ when filled with electrode solution.

    Article Title: α 1G T-type calcium channel determines the angiogenic potential of pulmonary microvascular endothelial cells
    Article Snippet: Recording pipettes were made of capillary corning glass tubing (Warner Instrument, Hamden, CT), pulled by a two-stage puller (PC-10; Narishige, Tokyo, Japan) and heat-polished with a microforge (MF-200; World Precision Instruments, Sarasota, FL).

    Article Title: Statin therapy exacerbates alcohol-induced constriction of cerebral arteries via modulation of ethanol-induced BK channel inhibition in vascular smooth muscle
    Article Snippet: Immediately before recording, the tip of each electrode was fire-polished on a microforge WPI MF-200 (World Precision Instruments, Sarasota, FL) to give resistances of 5–9 MΩ when filled with electrode solution.

    Article Title: Caribbean maitotoxin elevates [Ca 2+ ] i and activates non-selective cation channels in HIT-T15 cells
    Article Snippet: Patch pipettes were pulled by a two-stage puller (PC-10; Narishige, Greenvale, NY), and heat-polished with a microforge (MF-200; World Precision Instruments, Sarasota, FL) before use.

    Control:

    Article Title: Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts
    Article Snippet: .. EQUIPMENT list-behavior=unorderedprefix-word=mark-type=discmax-label-size=0 Glass rod (Narishige, cat. no. G-1000) Glass capillary puller (Narishige, cat. no. PC-10) Microforge (World Precision Instruments, cat. no. MF-200) (an instrument designed to fabricate the tips of glass capillaries and microneedles) Platinum heating filament (World Precision Instruments, cat. no. MF200-H4) Three-axis micromanipulators (Narishige, cat. no. MHW-3 or equivalent) ×2 ▲ CRITICAL The micromanipulators should allow for both coarse and fine movement control. .. Also, they should be equipped with low-drift or drift-free mechanisms so as to minimize drifting of the microneedles during the measurement, which is particularly important for obtaining data on minute time-scales. list-behavior=unorderedprefix-word=mark-type=discmax-label-size=0 Capillary holder ×2 Platinum wire (Omega Engineering, cat. no. SPPL-001); diameter ~25 μm Forceps Micro scissors Gas microburner Weighing scale Piezo actuator (Physics Instruments, cat. no. P-841.10) Piezo controller (Physics Instruments, cat. no. E-665) ▲CRITICAL Other combinations of piezo actuators and their controllers can also be used, but they should be equipped with a closed-loop circuit by which the piezo actuator can be operated with high linearity and repeatability in response to the applied voltage.

    Article Title: Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts
    Article Snippet: .. Glass rod (Narishige, cat. no. G-1000) Glass capillary puller (Narishige, cat. no. PC-10) Microforge (World Precision Instruments, cat. no. MF-200) (an instrument designed to fabricate the tips of glass capillaries and microneedles) Platinum heating filament (World Precision Instruments, cat. no. MF200-H4) Three-axis micromanipulators (Narishige, cat. no. MHW-3 or equivalent) ×2 ▲ CRITICAL The micromanipulators should allow for both coarse and fine movement control. ..

    Article Title: Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts
    Article Snippet: DNA dye (SYTOX Green; Invitrogen, cat. no. S7020) Dye-labeled tubulin (X-rhodamine labeled, 5–10 mg ml − 1, stored at − 80 °C), prepared as described in ref. 32. .. A detailed protocol can also be found on the Mitchison laboratory’s website (http://mitchison.med. harvard.edu/protocols.html) Sperm nuclei (prepared from male Xenopus laevis, 2–3 × 104 μl − 1, stored at − 80 °C), prepared as described in ref. 10 Silicone-coating solution (Sigma, cat. no. SL-2) Mineral oil (Sigma, cat. no. M8410) Agarose (SeaKem, cat. no. 50004) Glass cleaning solution (chromic-sulfuric acid; Fisher, cat. no. SC88) Vaseline (Sigma, cat. no. 16415) Acetone (reagent grade) Cytochalasine D (Sigma, cat. no. C-8273) CaCl 2 HEPES KOH MgCl 2 KCl Sucrose (Sigma, cat. no. S0389) Deionized water EQUIPMENT Glass rod (Narishige, cat. no. G-1000) Glass capillary puller (Narishige, cat. no. PC-10) Microforge (World Precision Instruments, cat. no. MF-200; an instrument designed to fabricate the tips of glass capillaries and microneedles) Platinum heating filament (World Precision Instruments, cat. no. MF200-H4) Three-axis micromanipulators (×2; Narishige, cat. no. MHW-3 or equivalent) crItIcal The micromanipulators should allow for both coarse and finemovement control. ..

    Electron Microscopy:

    Article Title: Viscoelasticity Measurements Reveal Rheological Differences Between Stem-like and Non-stem-like Breast Cancer Cells
    Article Snippet: 36 , 37 Briefly, micropipettes with tip inner diameters (ID) from 8 to 14 μm were pulled from 0.9 mm glass tubes (Friedrich & Dimmock, Millville, NJ) using a vertical puller (Model 730, David Kopf Instruments, Tujunga, CA). .. The tips were smoothed with a microforge (MF-200; World Precision Instruments, Sarasota, FL) and were measured by insertion of gold-coated glass microneedles that had been calibrated with scanning electron microscopy. ..



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