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centrifuge  (Eppendorf AG)


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    Eppendorf AG centrifuge
    Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 200034 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarrayer+2470+arrayer/Eppendorf/pm18974738-143-103-104
    Average 99 stars, based on 200034 article reviews
    centrifuge - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Gradient Centrifugation:

    Article Title: Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose
    Article Snippet: .. To purify macrophage cells, we next performed a density gradient centrifugation using OptiprepTM (Axis-Shield; 0%, 11.2%, 17.6%, and 24%) by centrifuging at 1400× g at room temperature for 20 min. After that, the fraction between 11.2 and 17.6% was gently collected using a transfer pipette (Eppendorf), washed 3 times with 1 × HBSS buffer, and resuspended in HBSS for FACS analysis. .. For cell sorting (FACS) analysis, the single-cell populations were incubated with a mouse Fc blocker (AAT Bioquest) for 20 min on ice, followed by staining with anti-CD45 (1:200, BD Biosciences).

    Transferring:

    Article Title: Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose
    Article Snippet: .. To purify macrophage cells, we next performed a density gradient centrifugation using OptiprepTM (Axis-Shield; 0%, 11.2%, 17.6%, and 24%) by centrifuging at 1400× g at room temperature for 20 min. After that, the fraction between 11.2 and 17.6% was gently collected using a transfer pipette (Eppendorf), washed 3 times with 1 × HBSS buffer, and resuspended in HBSS for FACS analysis. .. For cell sorting (FACS) analysis, the single-cell populations were incubated with a mouse Fc blocker (AAT Bioquest) for 20 min on ice, followed by staining with anti-CD45 (1:200, BD Biosciences).

    Article Title: Enzyme responsive antimicrobial hyaluronan-nanocellulose hybrid wound dressings for the treatment of infected wounds
    Article Snippet: .. Protocol b) involved mixing the supernatant by gentle pipetting, then transferring of 200 μL of supernatant to a new Eppendorf tube, which was subsequently centrifuged (10 min, 10,000 rpm). .. 100 μL of the supernatant were then extracted and measured, before being returned to the second Eppendorf tube, vortexed and transferred back to the dressing-containing tube.

    FACS:

    Article Title: Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose
    Article Snippet: .. To purify macrophage cells, we next performed a density gradient centrifugation using OptiprepTM (Axis-Shield; 0%, 11.2%, 17.6%, and 24%) by centrifuging at 1400× g at room temperature for 20 min. After that, the fraction between 11.2 and 17.6% was gently collected using a transfer pipette (Eppendorf), washed 3 times with 1 × HBSS buffer, and resuspended in HBSS for FACS analysis. .. For cell sorting (FACS) analysis, the single-cell populations were incubated with a mouse Fc blocker (AAT Bioquest) for 20 min on ice, followed by staining with anti-CD45 (1:200, BD Biosciences).

    Amplification:

    Article Title: Immune fitness and biomarkers of immune function: Relationships with the oral and gut microbiome composition
    Article Snippet: .. Amplicon libraries were pooled in equal volumes using an epMotion 5075 automated liquid handling system (Eppendorf, Hamburg, Germany). .. The pooled library was purified using AMPure XP magnetic beads (Beckman Coulter, cat. no. A63881) at a 0.6 × (vol/vol) bead-to-sample ratio to remove primer dimers and short nonspecific fragments.

    Gentle:

    Article Title: Enzyme responsive antimicrobial hyaluronan-nanocellulose hybrid wound dressings for the treatment of infected wounds
    Article Snippet: .. Protocol b) involved mixing the supernatant by gentle pipetting, then transferring of 200 μL of supernatant to a new Eppendorf tube, which was subsequently centrifuged (10 min, 10,000 rpm). .. 100 μL of the supernatant were then extracted and measured, before being returned to the second Eppendorf tube, vortexed and transferred back to the dressing-containing tube.



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