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sarcomas  (Novus Biologicals)


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    Structured Review

    Novus Biologicals sarcomas
    Sarcomas, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray/Human+Various+Tissue+MicroArray+(Sarcoma)/pmc08319167-181-9-16
    Average 90 stars, based on 3 article reviews
    sarcomas - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Commercially available paraffin-embedded tissue array slides containing 79 human sarcomas and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax, Inc., USA) were purchased.. Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.. Finally, to examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax).. Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax). Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. 1F ). Together, these results show

    Article Title: Enrichment of the embryonic stem cell reprogramming factors Oct4, Nanog, Myc, and Sox2 in benign and malignant vascular tumors
    Article Snippet: Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).. The sarcoma tissue arrays were purchased from Novus Biologicals (#NBP2 = 30332).. For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.

    Immunofluorescence:

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Commercially available paraffin-embedded tissue array slides containing 79 human sarcomas and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax, Inc., USA) were purchased.. Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.. Finally, to examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax).. Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax). Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. 1F ). Together, these results show

    Article Title: Enrichment of the embryonic stem cell reprogramming factors Oct4, Nanog, Myc, and Sox2 in benign and malignant vascular tumors
    Article Snippet: Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).. The sarcoma tissue arrays were purchased from Novus Biologicals (#NBP2 = 30332).. For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.



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    Image Search Results


    BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell Microarray Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.

    Journal: iScience

    Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity

    doi: 10.1016/j.isci.2026.116022

    Figure Lengend Snippet: BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell Microarray Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.

    Article Snippet: The Retrogenix cell microarray was performed by Charles River Laboratories and included 6,105 full-length human proteins (plasma membrane proteins, secreted or a cell surface-tethered secreted proteins) and 400 human heterodimers.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Microarray, Flow Cytometry, Transfection, Positive Control