Expressing:Article Title: Slit2 Inhibits Breast Cancer Metastasis by Activating M1-Like Phagocytic and Antifibrotic Macrophages
Article Snippet: .. To analyze Slit2 expression in human samples, we purchased two tissue microarrays (TMA) having normal, invasive, and lymph node metastatic primary tumors from Imgenex (IMH-364 and BR1008). .. To study the correlation of Slit2 expression with macrophage abundance in tumors we used TMA generated byDepartment of Pathology at The Ohio State University (Columbus, OH).
Microarray:Article Title: TALEN-edited allogeneic inducible dual CAR T cells enable effective targeting of solid tumors while mitigating off-tumor toxicity
Article Snippet: .. Human tumor tissue and healthy tissue microarray slides were obtained from Novus Biologicals (NBP2-30234, NBP2-78082, NBP2-30232, NBP2-30233, NBP2-30189). .. Mouse tumors were fixed in 10% buffered formalin (Thermo Fisher Scientific) overnight and moved to 70% ethanol thereafter.
Article Title: Discoidin Domain Receptor Tyrosine Kinase 1 (DDR1) Is a Novel Therapeutic Target in Liposarcoma: A Tissue Microarray Study
Article Snippet: Background Liposarcoma is the most commonly diagnosed subtype of soft tissue sarcoma.. As these tumors often arise near vital organs and neurovascular structures, complete resection can be challenging; consequently, recurrence rates are high.. Additionally, available chemotherapeutic agents have shown limited benefit and substantial toxicities.
Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer.
Article Snippet: .. Immunohistochemical (IHC) staining of human PC tissue microarray Under IRB approval from the University of Wisconsin Carbone Cancer Center (UWCCC), CHD1 (Abcam 236287; Cambridge, UK), NSD2 (Abcam 106180), H3K36me2 (CST 2901S, RRID: AB_1030983; Danvers, MA, USA), EZH2 (Novus NSP2-29965; Chesterfield, MI, USA), and Ecadherin (Ventana 790-4497, RRID: AB_2877943; Oro Valley, AZ, USA) levels were measured in a tissue microarray (TMA) created from CRPC patient tumor samples (n = 48) [2,18]. ..
Article Title: ATR inhibition sensitizes liposarcoma to doxorubicin by increasing DNA damage
Article Snippet: .. Liposarcoma tissue microarray (TMA) and immunohistochemistry (IHC) assay The human liposarcoma TMA was purchased from Novus Biologicals, LLC (Littleton, CO, USA), and used in accordance with the policies of the institutional review board of the Partners Human Research Committee (IRB protocol #2007P-002464) as previously described [ 23 , 24 ]. ..
Article Title: MT1-MMP dependent repression of the tumor suppressor SPRY4 contributes to MT1-MMP driven melanoma cell motility.
Article Snippet: 10 cells per field for a total of three fields per sample, were highlighted and their movement followed over a 24 hours period and distance computed using the MetaMorph software. .. The Human Melanoma tissue microarray was purchased from IMGENEX (Cat# INH-369) and stained with an anti SPRY4 antibody from ABGEN. ..
Article Title: Staphylococcal Nuclease and Tudor Domain Containing 1 (SND1 Protein) Promotes Hepatocarcinogenesis by Inhibiting Monoglyceride Lipase (MGLL)
Article Snippet: .. Tissue Microarray and Immunostaining Human HCC tissue microarrays were obtained from Imgenex Corp. Two tissue microarrays were used as follows: one containing 40 primary HCC, 10 metastatic HCC, and 9 normal adjacent liver samples (Imgenex; IMH-360); and the other containing 46 primary HCC and 13 metastatic HCC (Imgenex; IMH-318). .. Immunostaining was performed using anti-SND1 antibody (rabbit polyclonal; 1:100; Prestige Antibodies® powered by Atlas antibodies from Sigma), anti-MGLL antibody (goat polyclonal; 1:250; Abcam), and anti-PCNA antibody (mouse monoclonal; 1:300; Cell Signaling) as described ( 16 ).
Immunohistochemical staining:Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer.
Article Snippet: .. Immunohistochemical (IHC) staining of human PC tissue microarray Under IRB approval from the University of Wisconsin Carbone Cancer Center (UWCCC), CHD1 (Abcam 236287; Cambridge, UK), NSD2 (Abcam 106180), H3K36me2 (CST 2901S, RRID: AB_1030983; Danvers, MA, USA), EZH2 (Novus NSP2-29965; Chesterfield, MI, USA), and Ecadherin (Ventana 790-4497, RRID: AB_2877943; Oro Valley, AZ, USA) levels were measured in a tissue microarray (TMA) created from CRPC patient tumor samples (n = 48) [2,18]. ..
Immunohistochemistry:Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer.
Article Snippet: .. Immunohistochemical (IHC) staining of human PC tissue microarray Under IRB approval from the University of Wisconsin Carbone Cancer Center (UWCCC), CHD1 (Abcam 236287; Cambridge, UK), NSD2 (Abcam 106180), H3K36me2 (CST 2901S, RRID: AB_1030983; Danvers, MA, USA), EZH2 (Novus NSP2-29965; Chesterfield, MI, USA), and Ecadherin (Ventana 790-4497, RRID: AB_2877943; Oro Valley, AZ, USA) levels were measured in a tissue microarray (TMA) created from CRPC patient tumor samples (n = 48) [2,18]. ..
Article Title: ATR inhibition sensitizes liposarcoma to doxorubicin by increasing DNA damage
Article Snippet: .. Liposarcoma tissue microarray (TMA) and immunohistochemistry (IHC) assay The human liposarcoma TMA was purchased from Novus Biologicals, LLC (Littleton, CO, USA), and used in accordance with the policies of the institutional review board of the Partners Human Research Committee (IRB protocol #2007P-002464) as previously described [ 23 , 24 ]. ..
Staining:Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer.
Article Snippet: .. Immunohistochemical (IHC) staining of human PC tissue microarray Under IRB approval from the University of Wisconsin Carbone Cancer Center (UWCCC), CHD1 (Abcam 236287; Cambridge, UK), NSD2 (Abcam 106180), H3K36me2 (CST 2901S, RRID: AB_1030983; Danvers, MA, USA), EZH2 (Novus NSP2-29965; Chesterfield, MI, USA), and Ecadherin (Ventana 790-4497, RRID: AB_2877943; Oro Valley, AZ, USA) levels were measured in a tissue microarray (TMA) created from CRPC patient tumor samples (n = 48) [2,18]. ..
Article Title: MT1-MMP dependent repression of the tumor suppressor SPRY4 contributes to MT1-MMP driven melanoma cell motility.
Article Snippet: 10 cells per field for a total of three fields per sample, were highlighted and their movement followed over a 24 hours period and distance computed using the MetaMorph software. .. The Human Melanoma tissue microarray was purchased from IMGENEX (Cat# INH-369) and stained with an anti SPRY4 antibody from ABGEN. ..
Immunostaining:Article Title: Staphylococcal Nuclease and Tudor Domain Containing 1 (SND1 Protein) Promotes Hepatocarcinogenesis by Inhibiting Monoglyceride Lipase (MGLL)
Article Snippet: .. Tissue Microarray and Immunostaining Human HCC tissue microarrays were obtained from Imgenex Corp. Two tissue microarrays were used as follows: one containing 40 primary HCC, 10 metastatic HCC, and 9 normal adjacent liver samples (Imgenex; IMH-360); and the other containing 46 primary HCC and 13 metastatic HCC (Imgenex; IMH-318). .. Immunostaining was performed using anti-SND1 antibody (rabbit polyclonal; 1:100; Prestige Antibodies® powered by Atlas antibodies from Sigma), anti-MGLL antibody (goat polyclonal; 1:250; Abcam), and anti-PCNA antibody (mouse monoclonal; 1:300; Cell Signaling) as described ( 16 ).
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