gc tissue microarray chip (Proteintech)

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Proteintech gc tissue microarray chip
Gc Tissue Microarray Chip, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+tissue+chips/STUB1+Antibody/pmc12436174-80-5-32
Average 96 stars, based on 239 article reviews

gc tissue microarray chip - by Bioz Stars, 2026-09

96/100 stars

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Chromatin Immunoprecipitation:

Article Title: PARP1 stabilizes FOXN3 to suppress pulmonary fibrosis through p38-related feedback regulation.
Article Snippet: .. For ChIP quantitative PCR, the following antibodies were used: anti- Smad2 (no. 12570- 1- AP, Proteintech), anti- Smad3 (no. 66516- 1- Ig, Proteintech), anti- Smad4 (no. 10231- 1- AP, Proteintech), and anti- FOXN3 (no. ab129453, Abcam). ..

Article Title: PARP1 stabilizes FOXN3 to suppress pulmonary fibrosis through p38-related feedback regulation
Article Snippet: .. For ChIP quantitative PCR, the following antibodies were used: anti-Smad2 (no. 12570-1-AP, Proteintech), anti-Smad3 (no. 66516-1-Ig, Proteintech), anti-Smad4 (no. 10231-1-AP, Proteintech), and anti-FOXN3 (no. ab129453, Abcam). ..

Article Title: The transcription factor CUX1 exerts opposing roles in human and mouse adipocyte differentiation
Article Snippet: A 10 % aliquot of each sheared chromatin sample was reserved as input control, reverse crosslinked, and purified using column-based cleanup (NucleoSpin, Takara). .. For immunoprecipitation samples, chromatin was incubated overnight at 4 °C with CUX1 antibody (11733-1-AP, Proteintech) in ChIP buffer (1 % Triton X-100, 0.1 % SDS, 150 mM NaCl, 2 mM EDTA, 20 mM Tris–HCl, pH 8.0) supplemented with protease inhibitors. .. Antibody-bound complexes were captured using Protein A/G magnetic beads (Thermo Fisher Scientific), washed sequentially with wash buffers at increasingly higher salt concentrations (0.1 % SDS, 0.1 % NaDOC, 1 % Triton-100, 1 mM EDTA, 20 mM Tris HCl, 150 mM NaCl or 500 mM NaCl, pH 8.0), 0.5 % NP-40 and 250 mM LiCl, followed by elution in ChIP elution buffer (1 % SDS, 0.1 M NaHCO 3 ).

Article Title: Early growth response 1 promotes RNA polymerase I-directed transcription and cancer growth by activating RRN3 expression.
Article Snippet: .. The anti-RPA43 antibody used for ChIP assays was purchased from the Proteintech Co. (Cat no. 22784-1-AP, Wuhan, China). .. Ten micrograms of antibodies against TFIIA/ (Customed from Frdbio), TFIIB (SC-225, Santa Cruz Biotech), the Pol II RPB1 subunit (ab76123, Abcam) and TBP (SC-421 Santa Cruz Biotech) were used for a ChIP assay for individual Pol II machinery factors.

Real-time Polymerase Chain Reaction:

Immunoprecipitation:

Incubation:

Article Title: STUB1-mediated PGC-1α ubiquitination promotes postoperative cognitive dysfunction in aged mice via modulating autophagy-lysosome machinery.
Article Snippet: .. Incubation with primary antibodies of STUB1 (1:1000, 68407-1-Ig, Proteintech), PGC-1α (1:1000, 66369-1-Ig, Proteintech), TFEB (1:1000, 13372-1-AP, Proteintech), PINK1 (1:1000, ab300623, Abcam), PARKIN (1:1000, 14060-1-AP, Proteintech), LAMP-1 (1:1000, 21997-1-AP, Proteintech), Cathepsin D (1:1000, 21327-1-AP, Proteintech), Caspase3 (1:1000, 19677-1-AP, Proteintech), ubiquitin (1:1000, 10201-2-AP, Proteintech), and GAPDH (1:5000, 60004-1-Ig, Proteintech) was conducted at 4°C overnight. .. After rinsing by TBST (3 times×1 minutes), incubation with HRP-labeled goat anti-rabbit or anti-mouse IgG (1:5000, ZB2301 or ZB2305, ZSGB-BIO) was conducted at 37°C for 1 hour.

Western Blot:

Article Title: Reversible ubiquitination regulates HSF1 phase separation in response to proteotoxic stress.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Pyrolysis Gas Chromatography:

Ubiquitin Proteomics:

other:

Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins
Article Snippet: Anti-ACTB/β-actin antibody (66009-1-Ig), anti-GST antibody (HRP-66001), anti-STUB1 antibody (68407-1-Ig), anti-TOLLIP antibody (68170-1-Ig), horseradish peroxidase-labeled anti-rabbit and mouse antibodies, and IgG antibodies (SA00001-1, SA00001-2) were supplied by Proteintech.

Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins.
Article Snippet: Anti-ACTB/β-actin antibody (66009-1-Ig), anti-GST antibody (HRP-66001), anti-STUB1 antibody (68407-1-Ig), anti-TOLLIP antibody (68170-1-Ig), horseradish peroxidase-labeled anti-rabbit and mouse antibodies, and IgG antibodies (SA00001-1, SA00001-2) were supplied by Proteintech.

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