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tissue microarray  (PROVITRO GmbH)

 
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    PROVITRO GmbH tissue microarray
    Effects of WWP1 knockdown on cell proliferation, migration, and invasion in PCa cells and expression of WWP1 in clinical PCa specimens. ( A ) Cell proliferation was determined by XTT assays. ( B ) Cell migration activity was determined using uncoated Transwell polycarbonate membrane filters. ( C ) Cell invasion activity was determined by Matrigel invasion assays. ( D ) Representative image of IHC of WWP1 in the tissue <t>microarray.</t> ( E ) WWP1 was upregulated in PCa compared with PIN and normal tissue in the tissue microarray. * P <0.0001. The bars indicate s.d.'s.
    Tissue Microarray, supplied by PROVITRO GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+suite%2C+version+4%2E01/tissue+microarray/pmc04865980-85-1-6
    Average 90 stars, based on 1 article reviews
    tissue microarray - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Regulation of E3 ubiquitin ligase-1 ( WWP1 ) by microRNA-452 inhibits cancer cell migration and invasion in prostate cancer"

    Article Title: Regulation of E3 ubiquitin ligase-1 ( WWP1 ) by microRNA-452 inhibits cancer cell migration and invasion in prostate cancer

    Journal: British Journal of Cancer

    doi: 10.1038/bjc.2016.95

    Effects of WWP1 knockdown on cell proliferation, migration, and invasion in PCa cells and expression of WWP1 in clinical PCa specimens. ( A ) Cell proliferation was determined by XTT assays. ( B ) Cell migration activity was determined using uncoated Transwell polycarbonate membrane filters. ( C ) Cell invasion activity was determined by Matrigel invasion assays. ( D ) Representative image of IHC of WWP1 in the tissue microarray. ( E ) WWP1 was upregulated in PCa compared with PIN and normal tissue in the tissue microarray. * P <0.0001. The bars indicate s.d.'s.
    Figure Legend Snippet: Effects of WWP1 knockdown on cell proliferation, migration, and invasion in PCa cells and expression of WWP1 in clinical PCa specimens. ( A ) Cell proliferation was determined by XTT assays. ( B ) Cell migration activity was determined using uncoated Transwell polycarbonate membrane filters. ( C ) Cell invasion activity was determined by Matrigel invasion assays. ( D ) Representative image of IHC of WWP1 in the tissue microarray. ( E ) WWP1 was upregulated in PCa compared with PIN and normal tissue in the tissue microarray. * P <0.0001. The bars indicate s.d.'s.

    Techniques Used: Knockdown, Migration, Expressing, Activity Assay, Membrane, Microarray

    Related Articles

    Expressing:

    Article Title: Therapeutic agent targeted to receptor protein, test agent, antibody that binds to receptor protein, and screening method for molecularly targeted drugs
    Article Snippet: .. The CHRNB2 expression and the NPTXR expression were analyzed by an immunohistochemical staining method by using a tissue microarray (by Provitro). ..

    Article Title: Therapeutic agent targeted to receptor protein, test agent, antibody that binds to receptor protein, and screening method for molecularly targeted drugs
    Article Snippet: .. Expression Profile in Cancers of Various Organs The CHRNB2 expression analysis and the NPTXR expression analysis in various cancers were performed by using a tissue microarray (by Provitro). ..

    Immunohistochemical staining:

    Article Title: Therapeutic agent targeted to receptor protein, test agent, antibody that binds to receptor protein, and screening method for molecularly targeted drugs
    Article Snippet: .. The CHRNB2 expression and the NPTXR expression were analyzed by an immunohistochemical staining method by using a tissue microarray (by Provitro). ..

    Article Title: Targeting the aryl hydrocarbon receptor (AhR) with BAY 2416964: a selective small molecule inhibitor for cancer immunotherapy
    Article Snippet: .. Immunohistochemical (IHC) staining of human tumor tissue microarrays Human formalin-fixed paraffin-embedded (FFPE) tumor tissue microarrays (TMA) representing tumors from the indications head and neck squamous cell carcinoma (HNSCC), non-small cell lung cancer (NSCLC), and colorectal cancer (CRC) were purchased from Provitro (Berlin, Germany). ..

    Staining:

    Article Title: Therapeutic agent targeted to receptor protein, test agent, antibody that binds to receptor protein, and screening method for molecularly targeted drugs
    Article Snippet: .. The CHRNB2 expression and the NPTXR expression were analyzed by an immunohistochemical staining method by using a tissue microarray (by Provitro). ..

    Article Title: Targeting the aryl hydrocarbon receptor (AhR) with BAY 2416964: a selective small molecule inhibitor for cancer immunotherapy
    Article Snippet: The cells were routinely cultured according to the manufacturer’s protocols, subjected to DNA fingerprinting and regularly tested to be free from Mycoplasma contamination using MycoAlert (Lonza). .. Human tumor tissue microarrays (TMA; Provitro, Berlin, Germany) representing head and neck squamous cell carcinoma (HNSCC), non-small cell lung cancer (NSCLC) and colorectal cancer (CRC) were stained for AhR using the primary monoclonal AhR antibody ab190797 (1:100, Abcam, Cambridge, UK), scanned and analyzed visually for AhR localization. ..

    Article Title: Targeting the aryl hydrocarbon receptor (AhR) with BAY 2416964: a selective small molecule inhibitor for cancer immunotherapy
    Article Snippet: .. Immunohistochemical (IHC) staining of human tumor tissue microarrays Human formalin-fixed paraffin-embedded (FFPE) tumor tissue microarrays (TMA) representing tumors from the indications head and neck squamous cell carcinoma (HNSCC), non-small cell lung cancer (NSCLC), and colorectal cancer (CRC) were purchased from Provitro (Berlin, Germany). ..

    Microarray:

    Article Title: Therapeutic agent targeted to receptor protein, test agent, antibody that binds to receptor protein, and screening method for molecularly targeted drugs
    Article Snippet: .. The CHRNB2 expression and the NPTXR expression were analyzed by an immunohistochemical staining method by using a tissue microarray (by Provitro). ..

    Article Title: ER O-glycosylation in synovial fibroblasts drives cartilage degradation
    Article Snippet: .. Patient samples: Human tissue microarray (Catalog number 401 3201) of synovial tissues was obtained from provitro AG (Berlin, Germany). ..

    Article Title: Therapeutic agent targeted to receptor protein, test agent, antibody that binds to receptor protein, and screening method for molecularly targeted drugs
    Article Snippet: .. Expression Profile in Cancers of Various Organs The CHRNB2 expression analysis and the NPTXR expression analysis in various cancers were performed by using a tissue microarray (by Provitro). ..

    other:

    Article Title: Targeting the aryl hydrocarbon receptor (AhR) with BAY 2416964: a selective small molecule inhibitor for cancer immunotherapy.
    Article Snippet: Immunohistochemical staining of human tumor tissue microarrays Human tumor tissue microarrays (TMA; Provitro, Berlin, Germany) representing head and neck squamous cell carcinoma (HNSCC), non- small cell lung cancer (NSCLC) and colorectal cancer (CRC) were stained for AhR using the primary monoclonal AhR antibody ab190797 (1:100, Abcam, Cambridge, UK), scanned and analyzed visually for AhR localization.

    Article Title: ER O-glycosylation in synovial fibroblasts drives cartilage degradation.
    Article Snippet: Antibodies: Anti-CNX (ab10286, ab22595), anti-Vimentin (ab92547), anti-beta actin (ab8226), anti-GALNT2 (ab262868), anti-PDIA4 (ab82587, ab155800), anti-TGN46 (ab16059), anti-Giantin (ab37266), anti-PDIA3 (ab13506), anti-Collagen III (ab7778), anti-Fibronectin (ab2413), anti-Calreticulin (ab22683) and rabbit polyclonal isotype control (ab37415) antibodies were purchased from Abcam.

    Tissue:

    Article Title: Targeting the aryl hydrocarbon receptor (AhR) with BAY 2416964: a selective small molecule inhibitor for cancer immunotherapy
    Article Snippet: The cells were routinely cultured according to the manufacturer’s protocols, subjected to DNA fingerprinting and regularly tested to be free from Mycoplasma contamination using MycoAlert (Lonza). .. Human tumor tissue microarrays (TMA; Provitro, Berlin, Germany) representing head and neck squamous cell carcinoma (HNSCC), non-small cell lung cancer (NSCLC) and colorectal cancer (CRC) were stained for AhR using the primary monoclonal AhR antibody ab190797 (1:100, Abcam, Cambridge, UK), scanned and analyzed visually for AhR localization. ..

    Article Title: ER O-glycosylation in synovial fibroblasts drives cartilage degradation
    Article Snippet: .. Patient samples: Human tissue microarray (Catalog number 401 3201) of synovial tissues was obtained from provitro AG (Berlin, Germany). ..

    Article Title: ER O-glycosylation in synovial fibroblasts drives cartilage degradation
    Article Snippet: Formalin-fixed, paraffin-embedded tissue sections were deparaffinized in xylene substitute buffer (Sub-X, Leica Biosystems) and rehydrated. .. For mouse joint tissues and patient tissue microarray (provitro AG, Berlin, Germany), antigen retrieval was performed by immersion in Epitope Retrieval Solution pH 6 (Leica Biosystems) and incubated at 60°C in an oven for 18 hours. .. For human tissue specimens, antigen retrieval was performed using Epitope Retrieval Solution pH 6 (Leica Biosystems) in a pressure chamber (2100 Retriever, Akribis Scientific Limited, WA16 0JG, GB).

    Incubation:

    Article Title: ER O-glycosylation in synovial fibroblasts drives cartilage degradation
    Article Snippet: Formalin-fixed, paraffin-embedded tissue sections were deparaffinized in xylene substitute buffer (Sub-X, Leica Biosystems) and rehydrated. .. For mouse joint tissues and patient tissue microarray (provitro AG, Berlin, Germany), antigen retrieval was performed by immersion in Epitope Retrieval Solution pH 6 (Leica Biosystems) and incubated at 60°C in an oven for 18 hours. .. For human tissue specimens, antigen retrieval was performed using Epitope Retrieval Solution pH 6 (Leica Biosystems) in a pressure chamber (2100 Retriever, Akribis Scientific Limited, WA16 0JG, GB).

    Immunohistochemistry:

    Article Title: Targeting the aryl hydrocarbon receptor (AhR) with BAY 2416964: a selective small molecule inhibitor for cancer immunotherapy
    Article Snippet: .. Immunohistochemical (IHC) staining of human tumor tissue microarrays Human formalin-fixed paraffin-embedded (FFPE) tumor tissue microarrays (TMA) representing tumors from the indications head and neck squamous cell carcinoma (HNSCC), non-small cell lung cancer (NSCLC), and colorectal cancer (CRC) were purchased from Provitro (Berlin, Germany). ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Targeting the aryl hydrocarbon receptor (AhR) with BAY 2416964: a selective small molecule inhibitor for cancer immunotherapy
    Article Snippet: .. Immunohistochemical (IHC) staining of human tumor tissue microarrays Human formalin-fixed paraffin-embedded (FFPE) tumor tissue microarrays (TMA) representing tumors from the indications head and neck squamous cell carcinoma (HNSCC), non-small cell lung cancer (NSCLC), and colorectal cancer (CRC) were purchased from Provitro (Berlin, Germany). ..



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