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Grace Bio-Labs custom nitrocellulose coated microarray slides
Mean <t>microarray</t> antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
Custom Nitrocellulose Coated Microarray Slides, supplied by Grace Bio-Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+slide/coated+nitrocellulose+slides/pmc12977535-119-8-12
Average 86 stars, based on 1 article reviews
custom nitrocellulose coated microarray slides - by Bioz Stars, 2026-10
86/100 stars

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1) Product Images from "Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers"

Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

Journal: Journal of Clinical Microbiology

doi: 10.1128/jcm.01260-25

Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
Figure Legend Snippet: Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

Techniques Used: Microarray, Infection, Comparison

Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.
Figure Legend Snippet: Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

Techniques Used: Microarray, Infection, Comparison

Related Articles

Expressing:

Article Title: Human systemic and mucosal immune responses support further exploration of a Klebsiella pneumoniae protein-based vaccine
Article Snippet: The open reading frames were subcloned into a T7 expression vector pXI and expressed using an in vitro cell-free Escherichia coli transcription and cell-free translation (IVTT) system (Rapid Translation System, Biotechrabbit, Berlin, Germany). .. After expression, the proteins were printed onto nitrocellulose-coated AVID slides (Grace Bio-Labs Inc) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc., Marlborough, MA, USA). ..

Microarray:

Article Title: Human systemic and mucosal immune responses support further exploration of a Klebsiella pneumoniae protein-based vaccine
Article Snippet: The open reading frames were subcloned into a T7 expression vector pXI and expressed using an in vitro cell-free Escherichia coli transcription and cell-free translation (IVTT) system (Rapid Translation System, Biotechrabbit, Berlin, Germany). .. After expression, the proteins were printed onto nitrocellulose-coated AVID slides (Grace Bio-Labs Inc) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc., Marlborough, MA, USA). ..

Control:

Article Title: Loss of ZNRF3/RNF43 unleashes EGFR in cancer
Article Snippet: .. The Aushon 2470 Arrayer (Aushon BioSystems) with a 40 pin (185 μm) configuration was used to spot samples and control lysates onto nitrocellulose-coated slides (Grace Bio-labs) using an array format of 960 lysates/slide (2880 spots/slide). .. The slides were probed with a set of 216 antibodies against total and phospho-proteins using an automated slide stainer Autolink 48 (Dako).



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Mean <t>microarray</t> antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
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Mean <t>microarray</t> antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
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Mean <t>microarray</t> antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
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Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

Journal: Journal of Clinical Microbiology

Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

doi: 10.1128/jcm.01260-25

Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

Techniques: Microarray, Infection, Comparison

Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

Journal: Journal of Clinical Microbiology

Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

doi: 10.1128/jcm.01260-25

Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

Techniques: Microarray, Infection, Comparison