microarray slides (Corning Life Sciences)
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Microarray Slides, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+slide+hybridization/microarray+slides/pmc02607173-48-28-30
Average 90 stars, based on 1 article reviews
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Purification:Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells Article Snippet: Probes were then labeled with N -hydroxy-succinimide ester dye Cy3 or Cy5 (Amersham). .. After denaturation, purified Cy3/Cy5-labeled cDNA samples were combined and hybridized on a Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the Reverse Transcription:Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). .. RNA was then reverse transcribed to cDNA using Superscript III (Invitrogen), random primers (Invitrogen), and the incorporation ofaminoallyl-DUTP, 5-(3-aminoallyl)-2’-deoxyuridine 5’-triphosphate (Ambion, Austin, TX), as described previously ( Chou et al. 2007 ). cDNA was labeled with either Cy3 or Cy5 dye (GE Healthcare, Little Chalfont, United Kingdom), and hybridized to one of six Labeling:Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the Article Title: Conversion of 4-Hydroxybutyrate to Acetyl Coenzyme A and Its Anapleurosis in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Carbon Fixation Pathway Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse transcribed with Superscript III (Invitrogen), repurified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). .. RNA was then reverse transcribed to cDNA using Superscript III (Invitrogen), random primers (Invitrogen), and the incorporation ofaminoallyl-DUTP, 5-(3-aminoallyl)-2’-deoxyuridine 5’-triphosphate (Ambion, Austin, TX), as described previously ( Chou et al. 2007 ). cDNA was labeled with either Cy3 or Cy5 dye (GE Healthcare, Little Chalfont, United Kingdom), and hybridized to one of six Microarray:Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells Article Snippet: Probes were then labeled with N -hydroxy-succinimide ester dye Cy3 or Cy5 (Amersham). .. After denaturation, purified Cy3/Cy5-labeled cDNA samples were combined and hybridized on a Article Title: Conversion of 4-Hydroxybutyrate to Acetyl Coenzyme A and Its Anapleurosis in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Carbon Fixation Pathway Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse transcribed with Superscript III (Invitrogen), repurified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the Article Title: Sequestration of carbon dioxide with hydrogen to useful products Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). .. RNA was then reverse transcribed to cDNA using Superscript III (Invitrogen), random primers (Invitrogen), and the incorporation ofaminoallyl-DUTP, 5-(3-aminoallyl)-2’-deoxyuridine 5’-triphosphate (Ambion, Austin, TX), as described previously ( Chou et al. 2007 ). cDNA was labeled with either Cy3 or Cy5 dye (GE Healthcare, Little Chalfont, United Kingdom), and hybridized to one of six Saline:Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells Article Snippet: Probes were then labeled with N -hydroxy-succinimide ester dye Cy3 or Cy5 (Amersham). .. After denaturation, purified Cy3/Cy5-labeled cDNA samples were combined and hybridized on a other:Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). |
![(A) HIF-1α, HIF-2α, PHD2, and PHD3 protein levels were analyzed after cultivating BMDM for 24 h at 20% or 1% O2. (B) The expression of HIF-1 target genes, such as PKM2, Pfk, and PDK-1, on mRNA level was analyzed after cultivating BMDM for 24 h at 20% O2 or 1% O2 using qRT-PCR. (C) PHD3−/− [knockout (KO)] and WT BMDM were cultivated for 48 h at 20% O2 and 1% O2. Subsequently, cells were lysed and RNA isolated. RNA samples were analyzed in a transcriptome <t>microarray</t> assay. The volcano blots were generated based on the microarray results. (D) PHD3−/− and WT BMDM were cultured with or without serum for 24 h or stimulated with 100 ng LPS for 12 h. Subsequently, NF-κB activities were analyzed. As a positive control for the NF-κB activity assay, a cell extract of HeLa cells, which were treated with LPS, was used; n = 3; mean ± sd; **P < 0.01, and ***P < 0.001. rel. LU, Relative light units.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5934/pmc05395934/pmc05395934__zgb0071461060002.jpg)