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Corning Life Sciences microarray slides
Microarray Slides, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+slide+hybridization/microarray+slides/pmc02607173-48-28-30
Average 90 stars, based on 1 article reviews
microarray slides - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Purification:

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). ..

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells
Article Snippet: Probes were then labeled with N -hydroxy-succinimide ester dye Cy3 or Cy5 (Amersham). .. After denaturation, purified Cy3/Cy5-labeled cDNA samples were combined and hybridized on a microarray slide in a humidified chamber (Corning Life Sciences) at 65°C overnight in the presence of 5× saline-sodium citrate (SSC), 0.1% SDS, 25% formamide and polyA (25 ng/ μ l). .. Fluorescence-labeled cDNA was synthesized from 10 μ g of RNA from treated or untreated PC12 cells, with the SuperScript First Strand Synthesis System for RT-PCR (Invitrogen) in the presence of amine-modified random primers and aminoallyl-dUTP/dNTP.

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). .. Formation of the CoA ester was also confirmed using HPLC (Waters).

Reverse Transcription:

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). ..

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). .. Formation of the CoA ester was also confirmed using HPLC (Waters).

Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology
Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). .. RNA was then reverse transcribed to cDNA using Superscript III (Invitrogen), random primers (Invitrogen), and the incorporation ofaminoallyl-DUTP, 5-(3-aminoallyl)-2’-deoxyuridine 5’-triphosphate (Ambion, Austin, TX), as described previously ( Chou et al. 2007 ). cDNA was labeled with either Cy3 or Cy5 dye (GE Healthcare, Little Chalfont, United Kingdom), and hybridized to one of six microarray slides (Corning, Acton, MA) in the loop design (see Figure S1 ), following protocols previously described( Chou et al. 2007 ). .. Slides were scanned using an Axon scanner (Sunnyvale, CA) and quantified using GenePix Pro, version 6.0 software.

Labeling:

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). ..

Article Title: Conversion of 4-Hydroxybutyrate to Acetyl Coenzyme A and Its Anapleurosis in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Carbon Fixation Pathway
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse transcribed with Superscript III (Invitrogen), repurified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42°C. .. Slides were scanned on a GenePix 4000B microarray scanner (Molecular Devices, Sunnyvale, CA), and raw intensities were quantitated using GenePix pro version 6.0.

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). .. Formation of the CoA ester was also confirmed using HPLC (Waters).

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42° C. Slides were scanned on a GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, Calif.) and raw intensities were quantitated using GenePix Pro version 6.0. .. Data normalization and statistical analysis were performed using JMP Genomics 5 (SAS, Cary, N.C.).

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42° C. Slides were scanned on a GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, Calif.) and raw intensities were quantitated using GenePix Pro version 6.0. .. Data normalization and statistical analysis were performed using JMP Genomics 5 (SAS, Cary, N.C.).

Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology
Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). .. RNA was then reverse transcribed to cDNA using Superscript III (Invitrogen), random primers (Invitrogen), and the incorporation ofaminoallyl-DUTP, 5-(3-aminoallyl)-2’-deoxyuridine 5’-triphosphate (Ambion, Austin, TX), as described previously ( Chou et al. 2007 ). cDNA was labeled with either Cy3 or Cy5 dye (GE Healthcare, Little Chalfont, United Kingdom), and hybridized to one of six microarray slides (Corning, Acton, MA) in the loop design (see Figure S1 ), following protocols previously described( Chou et al. 2007 ). .. Slides were scanned using an Axon scanner (Sunnyvale, CA) and quantified using GenePix Pro, version 6.0 software.

Microarray:

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). ..

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells
Article Snippet: Probes were then labeled with N -hydroxy-succinimide ester dye Cy3 or Cy5 (Amersham). .. After denaturation, purified Cy3/Cy5-labeled cDNA samples were combined and hybridized on a microarray slide in a humidified chamber (Corning Life Sciences) at 65°C overnight in the presence of 5× saline-sodium citrate (SSC), 0.1% SDS, 25% formamide and polyA (25 ng/ μ l). .. Fluorescence-labeled cDNA was synthesized from 10 μ g of RNA from treated or untreated PC12 cells, with the SuperScript First Strand Synthesis System for RT-PCR (Invitrogen) in the presence of amine-modified random primers and aminoallyl-dUTP/dNTP.

Article Title: Conversion of 4-Hydroxybutyrate to Acetyl Coenzyme A and Its Anapleurosis in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Carbon Fixation Pathway
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse transcribed with Superscript III (Invitrogen), repurified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42°C. .. Slides were scanned on a GenePix 4000B microarray scanner (Molecular Devices, Sunnyvale, CA), and raw intensities were quantitated using GenePix pro version 6.0.

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). .. Formation of the CoA ester was also confirmed using HPLC (Waters).

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42° C. Slides were scanned on a GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, Calif.) and raw intensities were quantitated using GenePix Pro version 6.0. .. Data normalization and statistical analysis were performed using JMP Genomics 5 (SAS, Cary, N.C.).

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42° C. Slides were scanned on a GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, Calif.) and raw intensities were quantitated using GenePix Pro version 6.0. .. Data normalization and statistical analysis were performed using JMP Genomics 5 (SAS, Cary, N.C.).

Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology
Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). .. RNA was then reverse transcribed to cDNA using Superscript III (Invitrogen), random primers (Invitrogen), and the incorporation ofaminoallyl-DUTP, 5-(3-aminoallyl)-2’-deoxyuridine 5’-triphosphate (Ambion, Austin, TX), as described previously ( Chou et al. 2007 ). cDNA was labeled with either Cy3 or Cy5 dye (GE Healthcare, Little Chalfont, United Kingdom), and hybridized to one of six microarray slides (Corning, Acton, MA) in the loop design (see Figure S1 ), following protocols previously described( Chou et al. 2007 ). .. Slides were scanned using an Axon scanner (Sunnyvale, CA) and quantified using GenePix Pro, version 6.0 software.

Saline:

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells
Article Snippet: Probes were then labeled with N -hydroxy-succinimide ester dye Cy3 or Cy5 (Amersham). .. After denaturation, purified Cy3/Cy5-labeled cDNA samples were combined and hybridized on a microarray slide in a humidified chamber (Corning Life Sciences) at 65°C overnight in the presence of 5× saline-sodium citrate (SSC), 0.1% SDS, 25% formamide and polyA (25 ng/ μ l). .. Fluorescence-labeled cDNA was synthesized from 10 μ g of RNA from treated or untreated PC12 cells, with the SuperScript First Strand Synthesis System for RT-PCR (Invitrogen) in the presence of amine-modified random primers and aminoallyl-dUTP/dNTP.

other:

Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology
Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA).



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(A) HIF-1α, HIF-2α, PHD2, and PHD3 protein levels were analyzed after cultivating BMDM for 24 h at 20% or 1% O2. (B) The expression of HIF-1 target genes, such as PKM2, Pfk, and PDK-1, on mRNA level was analyzed after cultivating BMDM for 24 h at 20% O2 or 1% O2 using qRT-PCR. (C) PHD3−/− [knockout (KO)] and WT BMDM were cultivated for 48 h at 20% O2 and 1% O2. Subsequently, cells were lysed and RNA isolated. RNA samples were analyzed in a transcriptome <t>microarray</t> assay. The volcano blots were generated based on the microarray results. (D) PHD3−/− and WT BMDM were cultured with or without serum for 24 h or stimulated with 100 ng LPS for 12 h. Subsequently, NF-κB activities were analyzed. As a positive control for the NF-κB activity assay, a cell extract of HeLa cells, which were treated with LPS, was used; n = 3; mean ± sd; **P < 0.01, and ***P < 0.001. rel. LU, Relative light units.
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(A) HIF-1α, HIF-2α, PHD2, and PHD3 protein levels were analyzed after cultivating BMDM for 24 h at 20% or 1% O2. (B) The expression of HIF-1 target genes, such as PKM2, Pfk, and PDK-1, on mRNA level was analyzed after cultivating BMDM for 24 h at 20% O2 or 1% O2 using qRT-PCR. (C) PHD3−/− [knockout (KO)] and WT BMDM were cultivated for 48 h at 20% O2 and 1% O2. Subsequently, cells were lysed and RNA isolated. RNA samples were analyzed in a transcriptome microarray assay. The volcano blots were generated based on the microarray results. (D) PHD3−/− and WT BMDM were cultured with or without serum for 24 h or stimulated with 100 ng LPS for 12 h. Subsequently, NF-κB activities were analyzed. As a positive control for the NF-κB activity assay, a cell extract of HeLa cells, which were treated with LPS, was used; n = 3; mean ± sd; **P < 0.01, and ***P < 0.001. rel. LU, Relative light units.

Journal: Journal of Leukocyte Biology

Article Title: Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions

doi: 10.1189/jlb.2HI1013-533R

Figure Lengend Snippet: (A) HIF-1α, HIF-2α, PHD2, and PHD3 protein levels were analyzed after cultivating BMDM for 24 h at 20% or 1% O2. (B) The expression of HIF-1 target genes, such as PKM2, Pfk, and PDK-1, on mRNA level was analyzed after cultivating BMDM for 24 h at 20% O2 or 1% O2 using qRT-PCR. (C) PHD3−/− [knockout (KO)] and WT BMDM were cultivated for 48 h at 20% O2 and 1% O2. Subsequently, cells were lysed and RNA isolated. RNA samples were analyzed in a transcriptome microarray assay. The volcano blots were generated based on the microarray results. (D) PHD3−/− and WT BMDM were cultured with or without serum for 24 h or stimulated with 100 ng LPS for 12 h. Subsequently, NF-κB activities were analyzed. As a positive control for the NF-κB activity assay, a cell extract of HeLa cells, which were treated with LPS, was used; n = 3; mean ± sd; **P < 0.01, and ***P < 0.001. rel. LU, Relative light units.

Article Snippet: The hybridization was then carried out on the Agilent microarray hybridization slide at 65°C for 17 h. All subsequent steps were followed as instructed by the Low Input Quick Amp Labeling protocol.

Techniques: Expressing, Quantitative RT-PCR, Knock-Out, Isolation, Microarray, Generated, Cell Culture, Positive Control, Activity Assay