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microarray slides  (DNA Chip Research Inc)

 
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    DNA Chip Research Inc microarray slides
    Microarray Slides, supplied by DNA Chip Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+slide+hybridization/microarray+slides/pm15186442-45-16-25
    Average 90 stars, based on 1 article reviews
    microarray slides - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Comprehensive gene expression analysis of the response to straight-chain alcohols in Saccharomyces cerevisiae using cDNA microarray.
    Article Snippet: The RNA extracted from untreated control or chemical-treated cells were labelled with Cy3-dUTP or Cy5-dUTP, respectively (Amersham Biosciences, Piscataway, NJ, USA). .. The fluorescent Cy3- or Cy5-labelled cDNA probes were combined after purification (Amersham Biosciences) and hybridized onto microarray slides containing probes for ca 6200 yeast ORFs (DNA Chip Research Inc., Yokohama, Japan) at 65 C overnight. ..

    Article Title: cDNA microarray analysis of interleukin-1beta-induced Japanese flounder Paralichthys olivaceus kidney cells
    Article Snippet: Interleukin-1b (IL-1b) cDNA of Japanese flounder was found to consist of 1329 bp, encoded 247 amino acid residues.. Among the fish IL-1b in the databases, the one with the highest identity of Japanese flounder IL-1b was that of seabass (62% identity).. The expression of IL-1b was induced by treatment with concanavalin A (ConA)/phorbol myristate acetate (PMA) and lipopolysaccharide.

    Microarray:

    Article Title: Comprehensive gene expression analysis of the response to straight-chain alcohols in Saccharomyces cerevisiae using cDNA microarray.
    Article Snippet: The RNA extracted from untreated control or chemical-treated cells were labelled with Cy3-dUTP or Cy5-dUTP, respectively (Amersham Biosciences, Piscataway, NJ, USA). .. The fluorescent Cy3- or Cy5-labelled cDNA probes were combined after purification (Amersham Biosciences) and hybridized onto microarray slides containing probes for ca 6200 yeast ORFs (DNA Chip Research Inc., Yokohama, Japan) at 65 C overnight. ..

    Article Title: cDNA microarray analysis of interleukin-1beta-induced Japanese flounder Paralichthys olivaceus kidney cells
    Article Snippet: Interleukin-1b (IL-1b) cDNA of Japanese flounder was found to consist of 1329 bp, encoded 247 amino acid residues.. Among the fish IL-1b in the databases, the one with the highest identity of Japanese flounder IL-1b was that of seabass (62% identity).. The expression of IL-1b was induced by treatment with concanavalin A (ConA)/phorbol myristate acetate (PMA) and lipopolysaccharide.

    Polymerase Chain Reaction:

    Article Title: cDNA microarray analysis of interleukin-1beta-induced Japanese flounder Paralichthys olivaceus kidney cells
    Article Snippet: Interleukin-1b (IL-1b) cDNA of Japanese flounder was found to consist of 1329 bp, encoded 247 amino acid residues.. Among the fish IL-1b in the databases, the one with the highest identity of Japanese flounder IL-1b was that of seabass (62% identity).. The expression of IL-1b was induced by treatment with concanavalin A (ConA)/phorbol myristate acetate (PMA) and lipopolysaccharide.

    Labeling:

    Article Title: cDNA microarray analysis of interleukin-1beta-induced Japanese flounder Paralichthys olivaceus kidney cells
    Article Snippet: Interleukin-1b (IL-1b) cDNA of Japanese flounder was found to consist of 1329 bp, encoded 247 amino acid residues.. Among the fish IL-1b in the databases, the one with the highest identity of Japanese flounder IL-1b was that of seabass (62% identity).. The expression of IL-1b was induced by treatment with concanavalin A (ConA)/phorbol myristate acetate (PMA) and lipopolysaccharide.

    Hybridization:

    Article Title: cDNA microarray analysis of interleukin-1beta-induced Japanese flounder Paralichthys olivaceus kidney cells
    Article Snippet: Interleukin-1b (IL-1b) cDNA of Japanese flounder was found to consist of 1329 bp, encoded 247 amino acid residues.. Among the fish IL-1b in the databases, the one with the highest identity of Japanese flounder IL-1b was that of seabass (62% identity).. The expression of IL-1b was induced by treatment with concanavalin A (ConA)/phorbol myristate acetate (PMA) and lipopolysaccharide.



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    Image Search Results


    (A) HIF-1α, HIF-2α, PHD2, and PHD3 protein levels were analyzed after cultivating BMDM for 24 h at 20% or 1% O2. (B) The expression of HIF-1 target genes, such as PKM2, Pfk, and PDK-1, on mRNA level was analyzed after cultivating BMDM for 24 h at 20% O2 or 1% O2 using qRT-PCR. (C) PHD3−/− [knockout (KO)] and WT BMDM were cultivated for 48 h at 20% O2 and 1% O2. Subsequently, cells were lysed and RNA isolated. RNA samples were analyzed in a transcriptome microarray assay. The volcano blots were generated based on the microarray results. (D) PHD3−/− and WT BMDM were cultured with or without serum for 24 h or stimulated with 100 ng LPS for 12 h. Subsequently, NF-κB activities were analyzed. As a positive control for the NF-κB activity assay, a cell extract of HeLa cells, which were treated with LPS, was used; n = 3; mean ± sd; **P < 0.01, and ***P < 0.001. rel. LU, Relative light units.

    Journal: Journal of Leukocyte Biology

    Article Title: Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions

    doi: 10.1189/jlb.2HI1013-533R

    Figure Lengend Snippet: (A) HIF-1α, HIF-2α, PHD2, and PHD3 protein levels were analyzed after cultivating BMDM for 24 h at 20% or 1% O2. (B) The expression of HIF-1 target genes, such as PKM2, Pfk, and PDK-1, on mRNA level was analyzed after cultivating BMDM for 24 h at 20% O2 or 1% O2 using qRT-PCR. (C) PHD3−/− [knockout (KO)] and WT BMDM were cultivated for 48 h at 20% O2 and 1% O2. Subsequently, cells were lysed and RNA isolated. RNA samples were analyzed in a transcriptome microarray assay. The volcano blots were generated based on the microarray results. (D) PHD3−/− and WT BMDM were cultured with or without serum for 24 h or stimulated with 100 ng LPS for 12 h. Subsequently, NF-κB activities were analyzed. As a positive control for the NF-κB activity assay, a cell extract of HeLa cells, which were treated with LPS, was used; n = 3; mean ± sd; **P < 0.01, and ***P < 0.001. rel. LU, Relative light units.

    Article Snippet: The hybridization was then carried out on the Agilent microarray hybridization slide at 65°C for 17 h. All subsequent steps were followed as instructed by the Low Input Quick Amp Labeling protocol.

    Techniques: Expressing, Quantitative RT-PCR, Knock-Out, Isolation, Microarray, Generated, Cell Culture, Positive Control, Activity Assay