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circrna expression microarray slide  (Arraystar inc)

 
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    Structured Review

    Arraystar inc circrna expression microarray slide
    circRNAs expression profiles detected by <t>microarray</t> in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a <t>circRNA.</t> The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups
    Circrna Expression Microarray Slide, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+slide+hybridization/circrna+microarray/pmc07008530-81-8-12
    Average 90 stars, based on 1 article reviews
    circrna expression microarray slide - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm"

    Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

    Journal: BMC Cardiovascular Disorders

    doi: 10.1186/s12872-020-01374-8

    circRNAs expression profiles detected by microarray in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a circRNA. The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups
    Figure Legend Snippet: circRNAs expression profiles detected by microarray in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a circRNA. The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups

    Techniques Used: Expressing, Microarray, Control

    Validation of six randomly selected dysregulated circRNAs by qRT-PCR. Each circRNA was evaluated at least three times and compared with the results of microarray. The Y axis indicates the fold change of AAA vs control of each circRNA
    Figure Legend Snippet: Validation of six randomly selected dysregulated circRNAs by qRT-PCR. Each circRNA was evaluated at least three times and compared with the results of microarray. The Y axis indicates the fold change of AAA vs control of each circRNA

    Techniques Used: Biomarker Discovery, Quantitative RT-PCR, Microarray, Control

    The predicted circRNA/miRNA interaction networks for six randomly selected circRNAs. a , b The red nodes indicate upregulated circRNAs. c - f The blue nodes represent downregulated circRNAs. The green nodes are five complementary binding miRNAs of each circRNA
    Figure Legend Snippet: The predicted circRNA/miRNA interaction networks for six randomly selected circRNAs. a , b The red nodes indicate upregulated circRNAs. c - f The blue nodes represent downregulated circRNAs. The green nodes are five complementary binding miRNAs of each circRNA

    Techniques Used: Binding Assay

    Related Articles

    Microarray:

    Article Title: CircFak promotes mechanical force-induced osteogenesis via FAK/AKT phosphorylation.
    Article Snippet: .. In this study, differentially expressed circRNAs were analyzed in osteoblasts subjected to mechanical force via Arraystar circRNA microarray sequencing. ..

    Article Title: Recent progress in tuberculosis diagnosis: insights into blood-based biomarkers and emerging technologies
    Article Snippet: circRNA , 2018 , hsa_circ_0001204, hsa_circ_0001747 , Plasma , aTB vs HC , Human CircRNA Array V2.0 , qRT-PCR , UP , 86.21% , 89.17% , 0.928(p < 0.001) , Preclinical , ( ) . .. circRNA , 2018 , hsa_circ_0001953; hsa_circ_0009024 , Plasma , aTB vs HC , Arraystar circRNA Microarray , qRT-PCR , UP , 72.50% , 96.00% , 0.915(p < 0.001) , Preclinical , ( ) . ..

    Article Title: Investigation of Circular RNA Expression Profiles in Ultrasound-guided Incomplete Radiofrequency Ablation Transplanted Tumor Models of Human Liver Cancer.
    Article Snippet: 1 Department of Medical Ultrasonics, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning 530021, Guangxi Zhuang Autonomous Region, P. R. China 2 Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Zhuang Autonomous Region, Nanning, Guangxi, P. R. China Abstract Background Abnormally expressed circular RNAs (circRNAs) are associated with many diseases and have important biological effects on the regulation of gene expression.. However, the circRNA expression profile in incomplete radiofrequency ablation (RFA)-treated liver cancer (LC) patients has not been characterized.. This study investigated the potential biological effects of differentially expressed (DE) circRNAs in an incomplete RFA-treated transplantation tumor model of human LC.

    Article Title: Comprehensive Analysis Identifies Hsa_circ_0058191 as a Potential Drug Resistance Target in Multiple Myeloma
    Article Snippet: .. A total of 360 up-regulated and 438 down-regulated circRNAs were detected in bortezomib-treatment-resistant MM patients through Arraystar CircRNA Microarray. ..

    Article Title: Transcriptome Analysis of Non-coding RNAs and mRNAs in the Dorsal Root Ganglion of Peripheral Nerve Injury-Induced Neuropathic Pain.
    Article Snippet: .. Cao et.al performed circRNA expression profile in spinal dorsal horn of CCI rats using Arraystar Rat circRNA Microarray. ..

    Sequencing:

    Article Title: CircFak promotes mechanical force-induced osteogenesis via FAK/AKT phosphorylation.
    Article Snippet: .. In this study, differentially expressed circRNAs were analyzed in osteoblasts subjected to mechanical force via Arraystar circRNA microarray sequencing. ..

    Clinical Proteomics:

    Article Title: Recent progress in tuberculosis diagnosis: insights into blood-based biomarkers and emerging technologies
    Article Snippet: circRNA , 2018 , hsa_circ_0001204, hsa_circ_0001747 , Plasma , aTB vs HC , Human CircRNA Array V2.0 , qRT-PCR , UP , 86.21% , 89.17% , 0.928(p < 0.001) , Preclinical , ( ) . .. circRNA , 2018 , hsa_circ_0001953; hsa_circ_0009024 , Plasma , aTB vs HC , Arraystar circRNA Microarray , qRT-PCR , UP , 72.50% , 96.00% , 0.915(p < 0.001) , Preclinical , ( ) . ..

    Quantitative RT-PCR:

    Article Title: Recent progress in tuberculosis diagnosis: insights into blood-based biomarkers and emerging technologies
    Article Snippet: circRNA , 2018 , hsa_circ_0001204, hsa_circ_0001747 , Plasma , aTB vs HC , Human CircRNA Array V2.0 , qRT-PCR , UP , 86.21% , 89.17% , 0.928(p < 0.001) , Preclinical , ( ) . .. circRNA , 2018 , hsa_circ_0001953; hsa_circ_0009024 , Plasma , aTB vs HC , Arraystar circRNA Microarray , qRT-PCR , UP , 72.50% , 96.00% , 0.915(p < 0.001) , Preclinical , ( ) . ..

    Labeling:

    Article Title: Non-coding RNAs, a double-edged sword in breast cancer prognosis.
    Article Snippet: .. Next, the circRNAs were labeled and hybridized onto Arraystar Human circRNA Arrays. ..

    Expressing:

    Article Title: Transcriptome Analysis of Non-coding RNAs and mRNAs in the Dorsal Root Ganglion of Peripheral Nerve Injury-Induced Neuropathic Pain.
    Article Snippet: .. Cao et.al performed circRNA expression profile in spinal dorsal horn of CCI rats using Arraystar Rat circRNA Microarray. ..



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    Image Search Results


    (A) HIF-1α, HIF-2α, PHD2, and PHD3 protein levels were analyzed after cultivating BMDM for 24 h at 20% or 1% O2. (B) The expression of HIF-1 target genes, such as PKM2, Pfk, and PDK-1, on mRNA level was analyzed after cultivating BMDM for 24 h at 20% O2 or 1% O2 using qRT-PCR. (C) PHD3−/− [knockout (KO)] and WT BMDM were cultivated for 48 h at 20% O2 and 1% O2. Subsequently, cells were lysed and RNA isolated. RNA samples were analyzed in a transcriptome microarray assay. The volcano blots were generated based on the microarray results. (D) PHD3−/− and WT BMDM were cultured with or without serum for 24 h or stimulated with 100 ng LPS for 12 h. Subsequently, NF-κB activities were analyzed. As a positive control for the NF-κB activity assay, a cell extract of HeLa cells, which were treated with LPS, was used; n = 3; mean ± sd; **P < 0.01, and ***P < 0.001. rel. LU, Relative light units.

    Journal: Journal of Leukocyte Biology

    Article Title: Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions

    doi: 10.1189/jlb.2HI1013-533R

    Figure Lengend Snippet: (A) HIF-1α, HIF-2α, PHD2, and PHD3 protein levels were analyzed after cultivating BMDM for 24 h at 20% or 1% O2. (B) The expression of HIF-1 target genes, such as PKM2, Pfk, and PDK-1, on mRNA level was analyzed after cultivating BMDM for 24 h at 20% O2 or 1% O2 using qRT-PCR. (C) PHD3−/− [knockout (KO)] and WT BMDM were cultivated for 48 h at 20% O2 and 1% O2. Subsequently, cells were lysed and RNA isolated. RNA samples were analyzed in a transcriptome microarray assay. The volcano blots were generated based on the microarray results. (D) PHD3−/− and WT BMDM were cultured with or without serum for 24 h or stimulated with 100 ng LPS for 12 h. Subsequently, NF-κB activities were analyzed. As a positive control for the NF-κB activity assay, a cell extract of HeLa cells, which were treated with LPS, was used; n = 3; mean ± sd; **P < 0.01, and ***P < 0.001. rel. LU, Relative light units.

    Article Snippet: The hybridization was then carried out on the Agilent microarray hybridization slide at 65°C for 17 h. All subsequent steps were followed as instructed by the Low Input Quick Amp Labeling protocol.

    Techniques: Expressing, Quantitative RT-PCR, Knock-Out, Isolation, Microarray, Generated, Cell Culture, Positive Control, Activity Assay