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NimbleGen Systems GmbH tiling microarrays
Tiling Microarrays, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+services/tiling+microarray+based+service/pmc02910248-75-24-31
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Article Title: Transcriptomic and Metabolomic Analysis of a Fusidic Acid-Selected fusA Mutant of Staphylococcus aureus
Article Snippet: Comparative-genome sequencing (CGS) was performed with the complete genomes of S. aureus SH1000 and SH10001st-2 using a tiling microarray-based service provided by NimbleGen Systems Inc. (Madison, WI, USA) that can identify up to 95% of all single nucleotide polymorphisms (SNPs) and insertion-deletions, followed by sequencing of the genomic alterations identified ( ).

Article Title: A novel heterozygous deletion within the 3' region of the PAX6 gene causing isolated aniridia in a large family group.
Article Snippet: a Department of Neurosurgery, Van Military Hospital, Van, Turkey b Department of Medical Genetics, Marmara University School of Medicine, Istanbul, Turkey c Department of Neurosurgery, Program on Neurogenetics, Yale University School of Medicine, New Haven, Connecticut, USA d Child Study Center, Yale University School of Medicine, New Haven, Connecticut, USA e Department of Ophthalmology, Van Military Hospital, Van, Turkey f Department of Genetics, Istanbul Surgery Hospital, Istanbul, Turkey g Department of Family Practice, Istanbul University School of Medicine, Istanbul, Turkey h W.M.. Keck Facility, Microarray Center, Yale University, New Haven, Connecticut, USA

Article Title: Genomic, Transcriptomic and Metabolomic Studies of Two Well-Characterized, Laboratory-Derived Vancomycin-Intermediate Staphylococcus aureus Strains Derived from the Same Parent Strain
Article Snippet: These were performed with an array-based service provided by Nimblegen Systems Inc. (Madison, WI, USA) [ , ].

Article Title: Transcriptomic and Metabolomic Analysis of a Fusidic Acid-Selected fusA Mutant of Staphylococcus aureus .
Article Snippet: Comparative-genome sequencing (CGS) was performed with the complete genomes of S. aureus SH1000 and SH10001st-2 using a tiling microarray-based service provided by NimbleGen Systems Inc. (Madison, WI, USA) that can identify up to 95% of all single nucleotide polymorphisms (SNPs) and insertion-deletions, followed by sequencing of the genomic alterations identified (Table 1).

Chromatin Immunoprecipitation:

Article Title: Histone Modifications at Human Enhancers Reflect Global Cell Type-Specific Gene Expression
Article Snippet: Passage 32 H1 cells were cultured as described 22 with/without 200ng/ml BMP4 for 6 days (RND systems). .. Chromatin preparation, ChIP, DNA purification, and LM-PCR were performed as described using commercially available and custom antibodies, and ChIP samples were hybridized to tiling microarrays and to custom condensed enhancer microarrays (NimbleGen Systems, Inc.) as described 5 , 6 . ..

DNA Purification:

Article Title: Histone Modifications at Human Enhancers Reflect Global Cell Type-Specific Gene Expression
Article Snippet: Passage 32 H1 cells were cultured as described 22 with/without 200ng/ml BMP4 for 6 days (RND systems). .. Chromatin preparation, ChIP, DNA purification, and LM-PCR were performed as described using commercially available and custom antibodies, and ChIP samples were hybridized to tiling microarrays and to custom condensed enhancer microarrays (NimbleGen Systems, Inc.) as described 5 , 6 . ..

Immunoprecipitation:

Article Title: Identification of two regulatory elements controlling Fucosyltransferase 7 transcription in murine CD4+ T cells.
Article Snippet: Fucosyltransferase VII encoded by the gene Fut7 is essential in CD4+ T cells for the generation of Eand P-selectin ligands (Eand P-lig) which facilitate recruitment of lymphocytes into inflamed tissues and into the skin.. This study aimed to identify regulatory elements controlling the inducible Fut7 expression in CD4+ T cells that occurs upon activation and differentiation of naive T cells into effector cells.. Comparative analysis of the histone modification pattern in non-hematopoetic cells and CD4+ T cell subsets revealed a differential histone modification pattern within the Fut7 locus including a conserved non-coding sequence (CNS) identified by cross-species conservation comparison suggesting that regulatory elements are confined to this region.

Amplification:

Article Title: Identification of two regulatory elements controlling Fucosyltransferase 7 transcription in murine CD4+ T cells.
Article Snippet: Fucosyltransferase VII encoded by the gene Fut7 is essential in CD4+ T cells for the generation of Eand P-selectin ligands (Eand P-lig) which facilitate recruitment of lymphocytes into inflamed tissues and into the skin.. This study aimed to identify regulatory elements controlling the inducible Fut7 expression in CD4+ T cells that occurs upon activation and differentiation of naive T cells into effector cells.. Comparative analysis of the histone modification pattern in non-hematopoetic cells and CD4+ T cell subsets revealed a differential histone modification pattern within the Fut7 locus including a conserved non-coding sequence (CNS) identified by cross-species conservation comparison suggesting that regulatory elements are confined to this region.

Microarray:

Article Title: Identification of two regulatory elements controlling Fucosyltransferase 7 transcription in murine CD4+ T cells.
Article Snippet: Fucosyltransferase VII encoded by the gene Fut7 is essential in CD4+ T cells for the generation of Eand P-selectin ligands (Eand P-lig) which facilitate recruitment of lymphocytes into inflamed tissues and into the skin.. This study aimed to identify regulatory elements controlling the inducible Fut7 expression in CD4+ T cells that occurs upon activation and differentiation of naive T cells into effector cells.. Comparative analysis of the histone modification pattern in non-hematopoetic cells and CD4+ T cell subsets revealed a differential histone modification pattern within the Fut7 locus including a conserved non-coding sequence (CNS) identified by cross-species conservation comparison suggesting that regulatory elements are confined to this region.

Article Title: Whole genome transcription profiling of Anaplasma phagocytophilum in human and tick host cells by tiling array analysis
Article Snippet: .. Through Affymetrix (Santa Clara, CA) and NimbleGen Systems, Inc. (Madison, WI), we designed a tiling microarray for the entire genome of Ap (1.47 Mbp) and characterized Ap gene transcription in three cell lines representative of its life cycle (ISE6 tick) and pathogenesis in humans (HL-60 and HMEC-1). .. Sterile and Ap -infected HL-60 cells (American Type Culture Collection, Manassas, VA, USA; ATCC CCL-240) were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 25 mM HEPES.



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CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

Journal: Frontiers in Immunology

Article Title: Altered circular RNA expressions in extracellular vesicles from bronchoalveolar lavage fluids in mice after bacterial infections

doi: 10.3389/fimmu.2024.1354676

Figure Lengend Snippet: CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

Article Snippet: For the circRNA profiling using microarrays, we used the service from Arraystar (Rockville, MD).

Techniques: Isolation