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mirna microarray service  (Annoroad Gene Technology Co Ltd)

 
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    Annoroad Gene Technology Co Ltd mirna microarray service
    Mirna Microarray Service, supplied by Annoroad Gene Technology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+services/mirna+microarray+service/pmc11441009-124-21-18
    Average 90 stars, based on 1 article reviews
    mirna microarray service - by Bioz Stars, 2026-09
    90/100 stars

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    Microarray:

    Article Title: Sbno1 mediates cell–cell communication between neural stem cells and microglia through small extracellular vesicles
    Article Snippet: MAbs of mice were as follows: APC-anti-CD11b, PE -anti-F4/80, PE-cy7-anti-CD206 (BioLegend, USA). .. Total RNA extracted from the NSCs and Sbno1-NSCs were used for miRNA arrays. miRNA profiles were performed with ANNOROAD GENOME’s (Beijing, China) miRNA microarray service based on Affymetrix miRNA 3.0 Array. ..



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    CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

    Journal: Frontiers in Immunology

    Article Title: Altered circular RNA expressions in extracellular vesicles from bronchoalveolar lavage fluids in mice after bacterial infections

    doi: 10.3389/fimmu.2024.1354676

    Figure Lengend Snippet: CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

    Article Snippet: For the circRNA profiling using microarrays, we used the service from Arraystar (Rockville, MD).

    Techniques: Isolation