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microarray services  (Fisher Scientific)

 
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    Fisher Scientific microarray services
    Microarray Services, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+services/microarray+analysis/pm38261515-291-9-6
    Average 90 stars, based on 1 article reviews
    microarray services - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: IL‐17A deficiency mitigates bleomycin‐induced complement activation during lung fibrosis
    Article Snippet: .. IL-17A activates C9 by modulating the mRNA and protein expression of the C9 components in vitro Microarray gene analyses (Affymetrix; Thermo Fisher Scientific) of normal primary human SAECs exposed to IL-17A for 24 h revealed a;5- and;1.75-fold induction of C3 and C9 factor B mRNA, respectively (Table 2; N = 5 donor lungs), and ;1.4-fold induction of GRK5. ..

    In Vitro:

    Article Title: IL‐17A deficiency mitigates bleomycin‐induced complement activation during lung fibrosis
    Article Snippet: .. IL-17A activates C9 by modulating the mRNA and protein expression of the C9 components in vitro Microarray gene analyses (Affymetrix; Thermo Fisher Scientific) of normal primary human SAECs exposed to IL-17A for 24 h revealed a;5- and;1.75-fold induction of C3 and C9 factor B mRNA, respectively (Table 2; N = 5 donor lungs), and ;1.4-fold induction of GRK5. ..

    Microarray:

    Article Title: IL‐17A deficiency mitigates bleomycin‐induced complement activation during lung fibrosis
    Article Snippet: .. IL-17A activates C9 by modulating the mRNA and protein expression of the C9 components in vitro Microarray gene analyses (Affymetrix; Thermo Fisher Scientific) of normal primary human SAECs exposed to IL-17A for 24 h revealed a;5- and;1.75-fold induction of C3 and C9 factor B mRNA, respectively (Table 2; N = 5 donor lungs), and ;1.4-fold induction of GRK5. ..

    Article Title: Circulating tumor cells reveal early predictors of disease progression in patients with stage III NSCLC undergoing chemoradiation and immunotherapy.
    Article Snippet: Affymetrix Clariom S PICO microarray processing After thawing gently on ice, 11mL of the RNA samples were plated into a sterile barcoded PCR plate supplied by Thermo Fisher. .. The samples were then shipped to Fisher scientific for microarray services by their lab. ..

    Article Title: IL-17A deficiency mitigates bleomycin-induced complement activation during lung fibrosis
    Article Snippet: .. Baseline and pulmonary function characteristics of IPF patients Microarray analysis SAECs were treated with IL-17A (100 ng/ml) for 24 h. RNA was isolated, and cDNA was subjected to microarray analysis (Affymetrix; Thermo Fisher Scientific, Waltham, MA, USA) by the Microarray Core (University of Texas Southwest Medical Center, Dallas, TX, USA). .. Real-time PCR Real-time PCR was performed on cDNA from cell lysates, as reported in several publications ( 3 , 13 , 26 , 30 , 31 ), by using gene-specific Taqman assays (Thermo Fisher Scientific).

    Article Title: An integrative proteomics method identifies a regulator of translation during stem cell maintenance and differentiation
    Article Snippet: .. The microarray analysis was performed according to the manufacturer’s instructions (Termo Fischer Scientific UserGuide P/N 703174). .. Procedures for cDNA synthesis, target DNA fragmentation, and labelling were carried out according to the GeneChip WT PLUS Reagent Kit (Applied Biosystems) using 500 ng of total RNA as the starting material.

    Article Title: Response of peripheral blood basophils in subjects with chronic spontaneous urticaria during treatment with omalizumab.
    Article Snippet: Background: Treatment of patients with asthma or food allergy with omalizumab results in several consistent changes in circulating basophils.. The multiple basophil phenotypes observed in patients with chronic spontaneous urticaria (CSU) present some unique attributes that may not respond in a similar fashion to patients with asthma or food allergy.. As part of a clinical study on the therapeutic outcomes of omalizumab treatment in CSU, the basophil compartment was examined for changes in characteristics predicted by prior studies.

    Isolation:

    Article Title: IL-17A deficiency mitigates bleomycin-induced complement activation during lung fibrosis
    Article Snippet: .. Baseline and pulmonary function characteristics of IPF patients Microarray analysis SAECs were treated with IL-17A (100 ng/ml) for 24 h. RNA was isolated, and cDNA was subjected to microarray analysis (Affymetrix; Thermo Fisher Scientific, Waltham, MA, USA) by the Microarray Core (University of Texas Southwest Medical Center, Dallas, TX, USA). .. Real-time PCR Real-time PCR was performed on cDNA from cell lysates, as reported in several publications ( 3 , 13 , 26 , 30 , 31 ), by using gene-specific Taqman assays (Thermo Fisher Scientific).

    Article Title: Response of peripheral blood basophils in subjects with chronic spontaneous urticaria during treatment with omalizumab.
    Article Snippet: Background: Treatment of patients with asthma or food allergy with omalizumab results in several consistent changes in circulating basophils.. The multiple basophil phenotypes observed in patients with chronic spontaneous urticaria (CSU) present some unique attributes that may not respond in a similar fashion to patients with asthma or food allergy.. As part of a clinical study on the therapeutic outcomes of omalizumab treatment in CSU, the basophil compartment was examined for changes in characteristics predicted by prior studies.



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    CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA <t>microarrays</t> by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.
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    Image Search Results


    CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

    Journal: Frontiers in Immunology

    Article Title: Altered circular RNA expressions in extracellular vesicles from bronchoalveolar lavage fluids in mice after bacterial infections

    doi: 10.3389/fimmu.2024.1354676

    Figure Lengend Snippet: CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

    Article Snippet: For the circRNA profiling using microarrays, we used the service from Arraystar (Rockville, MD).

    Techniques: Isolation