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cy5 streptavidin  (Danaher Inc)


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    Danaher Inc cy5 streptavidin
    Cy5 Streptavidin, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+scanner+arrayworx+e/Cy5-Streptavidin/pmc01779567-339-20-21
    Average 96 stars, based on 278 article reviews
    cy5 streptavidin - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Targeted Deposition of Antibodies on a Multiplex CMOS Microarray and Optimization of a Sensitive Immunoassay Using Electrochemical Detection
    Article Snippet: .. For fluorescence detection, Cy5-streptavidin (GE Healthcare, Amersham Biosciences, Piscataway, NJ) was added for 1 h, washed five times in PBSC and twice in PBS and scanned on a GenePix 4000B (Axon Instruments, Molecular Devices, Sunnyvale, CA). .. For ECD, microarrays were incubated for 30 min with Poly-80-HRP Streptavidin (Fitzgerald Industries International, Acton, MA) diluted 1∶1000 in PBSC.

    Microarray:

    Article Title: Consideration of Commercially Available Hepatocytes as Cell Sources for Liver-Microphysiological Systems by Comparing Liver Characteristics
    Article Snippet: .. The DNA microarray was labeled with streptavidin-Cy5 (GE Healthcare Bio-Science KK, Tokyo, Japan). .. Hybridization signals were acquired using a DNA microarray reader with multibeam excitation technology (Yokogawa Electric Co., Tokyo, Japan).

    Article Title: β-Cryptoxanthin Alleviates Diet-Induced Nonalcoholic Steatohepatitis by Suppressing Inflammatory Gene Expression in Mice
    Article Snippet: .. The microarray was then fluorescently labeled with streptavidin-Cy5 (GE Healthcare Japan, Tokyo, Japan) and washed in 0.12 M Tris-HCl/0.12 M NaCl/0.05% Tween 20 at room temperature and in 1 mL of 0.12 M Tris-HCl/0.12 M NaCl. .. Hybridization signals were acquired using a DNA microarray reader and multibeam excitation technology (Yokogawa Electric Co., Tokyo, Japan).

    Labeling:

    Article Title: Consideration of Commercially Available Hepatocytes as Cell Sources for Liver-Microphysiological Systems by Comparing Liver Characteristics
    Article Snippet: .. The DNA microarray was labeled with streptavidin-Cy5 (GE Healthcare Bio-Science KK, Tokyo, Japan). .. Hybridization signals were acquired using a DNA microarray reader with multibeam excitation technology (Yokogawa Electric Co., Tokyo, Japan).

    Article Title: β-Cryptoxanthin Alleviates Diet-Induced Nonalcoholic Steatohepatitis by Suppressing Inflammatory Gene Expression in Mice
    Article Snippet: .. The microarray was then fluorescently labeled with streptavidin-Cy5 (GE Healthcare Japan, Tokyo, Japan) and washed in 0.12 M Tris-HCl/0.12 M NaCl/0.05% Tween 20 at room temperature and in 1 mL of 0.12 M Tris-HCl/0.12 M NaCl. .. Hybridization signals were acquired using a DNA microarray reader and multibeam excitation technology (Yokogawa Electric Co., Tokyo, Japan).

    Article Title: Dysregulation of IFN System Can Lead to Poor Response to Pegylated Interferon and Ribavirin Therapy in Chronic Hepatitis C
    Article Snippet: .. After hybridization, Genopal was washed with hybridization buffer twice at 65°C for 20 min followed by washing in 0.12 M Tris-HCl/0.12 M NaCl at 65°C for 10 min. Genopal was then labeled with streptavidin-Cy5 (GE Healthcare Bioscience, Tokyo, Japan). .. The fluorescent labeled-Genopal was washed for 5 min four times with hybridization buffer at RT and scanned at multiple exposure times ranging from 0 to 40s by DNA microarray reader (Yokogawa Electric Co, Tokyo, Japan).

    Article Title: Shear stress-induced mechanotransduction protein deregulation and vasculopathy in a mouse model of progeria
    Article Snippet: .. Labeled Cy2-, Cy3- and Cy5- samples were loaded on an immobilized pH gradient strip (pH 3 to 10, GE Healthcare). ..

    Hybridization:

    Article Title: Dysregulation of IFN System Can Lead to Poor Response to Pegylated Interferon and Ribavirin Therapy in Chronic Hepatitis C
    Article Snippet: .. After hybridization, Genopal was washed with hybridization buffer twice at 65°C for 20 min followed by washing in 0.12 M Tris-HCl/0.12 M NaCl at 65°C for 10 min. Genopal was then labeled with streptavidin-Cy5 (GE Healthcare Bioscience, Tokyo, Japan). .. The fluorescent labeled-Genopal was washed for 5 min four times with hybridization buffer at RT and scanned at multiple exposure times ranging from 0 to 40s by DNA microarray reader (Yokogawa Electric Co, Tokyo, Japan).

    Stripping Membranes:

    Article Title: Shear stress-induced mechanotransduction protein deregulation and vasculopathy in a mouse model of progeria
    Article Snippet: .. Labeled Cy2-, Cy3- and Cy5- samples were loaded on an immobilized pH gradient strip (pH 3 to 10, GE Healthcare). ..



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    Anabaena circinalis 131C putative specific sequences with protein matches in The National Center for Biotechnology Information (NCBI) protein database
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    Image Search Results


    a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.

    Journal: PLoS ONE

    Article Title: A Microfluidic Platform for High-Throughput Multiplexed Protein Quantitation

    doi: 10.1371/journal.pone.0117744

    Figure Lengend Snippet: a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.

    Article Snippet: The microfluidic device was scanned using a fluorescent microarray scanner (ArrayWorx e-Biochip Reader, Applied Precision, USA).

    Techniques: Control, Labeling, Microarray

    Anabaena circinalis 131C putative specific sequences with protein matches in The National Center for Biotechnology Information (NCBI) protein database

    Journal:

    Article Title: PCR-based positive hybridization to detect genomic diversity associated with bacterial secondary metabolism

    doi: 10.1093/nar/gnh012

    Figure Lengend Snippet: Anabaena circinalis 131C putative specific sequences with protein matches in The National Center for Biotechnology Information (NCBI) protein database

    Article Snippet: Microarray scanning, data acquisition and statistical analyses Clean slides were scanned with the ArrayWorx ‘e’ Microarray Scanner from Applied Precision, Inc. (Issaquah, WA).

    Techniques: Microarray, Hybridization

    Anabaena circinalis 344B putative specific sequences with protein matches (Id: identity; Sim: similarity) in the NCBI protein database

    Journal:

    Article Title: PCR-based positive hybridization to detect genomic diversity associated with bacterial secondary metabolism

    doi: 10.1093/nar/gnh012

    Figure Lengend Snippet: Anabaena circinalis 344B putative specific sequences with protein matches (Id: identity; Sim: similarity) in the NCBI protein database

    Article Snippet: Microarray scanning, data acquisition and statistical analyses Clean slides were scanned with the ArrayWorx ‘e’ Microarray Scanner from Applied Precision, Inc. (Issaquah, WA).

    Techniques: Microarray, Hybridization