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Digilabs Inc omni grid accent robotic microarray
Omni Grid Accent Robotic Microarray, supplied by Digilabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+robot/omni+grid+accent+robotic+microarray/pmc10906480-269-21-27
Average 90 stars, based on 1 article reviews
omni grid accent robotic microarray - by Bioz Stars, 2026-11
90/100 stars

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Expressing:

Article Title: Sterile protection against human malaria by chemoattenuated PfSPZ vaccine
Article Snippet: Purified PfSPZ (NF54 strain) from aseptic Anopheles stephensi mosquitoes produced by Sanaria were resuspended in phosphate buffered saline (PBS (pH 7.4)) to obtain 5 × 10 3 PfSPZ per 40 μl 40 μl (5 × 10 3 PfSPZ) were added to each well of Greiner cellstar clear-bottom black 96-well plates (Sigma-Aldrich). .. After expressing the proteins according to manufacturer’s instructions, translated proteins were printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc.). ..

Article Title: Specific Cryptosporidium antigens associate with reinfection immunity and protection from cryptosporidiosis
Article Snippet: Briefly, the open reading frames selected as described above were subcloned into a T7 expression vector pXI and expressed using an in vitro the Escherichia coli transcription and cell free translation (IVTT) system (Rapid Translation System, Biotechrabbit). .. After expression the proteins were printed onto nitrocellulose-coated AVID slides (Grace Bio-Labs Inc.) using an Omni Grid Accent robotic microarray printer (Digilabs Inc.). ..

Article Title: Strong off-target antibody reactivity to malarial antigens induced by RTS,S/AS01E vaccination is associated with protection
Article Snippet: .. After expressing the proteins according to manufacturer instructions, translated proteins were printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs, Inc.) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc.). ..

Microarray:

Article Title: Sterile protection against human malaria by chemoattenuated PfSPZ vaccine
Article Snippet: Purified PfSPZ (NF54 strain) from aseptic Anopheles stephensi mosquitoes produced by Sanaria were resuspended in phosphate buffered saline (PBS (pH 7.4)) to obtain 5 × 10 3 PfSPZ per 40 μl 40 μl (5 × 10 3 PfSPZ) were added to each well of Greiner cellstar clear-bottom black 96-well plates (Sigma-Aldrich). .. After expressing the proteins according to manufacturer’s instructions, translated proteins were printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc.). ..

Article Title: Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray
Article Snippet: .. Arrays were printed onto nitrocellulose-coated slides (AVID, Grace Bio-Labs, Inc., Bend, OR, USA) using an Omni Grid Accent microarray printer (Digilabs, Inc., Marlborough, MA, USA). .. Alongside proteins of interest, buffer (PBS) and no-DNA (empty T7 vector reactions) were included as controls to allow for background normalisation of purified and IVTT proteins respectively.

Article Title: Specific Cryptosporidium antigens associate with reinfection immunity and protection from cryptosporidiosis
Article Snippet: Briefly, the open reading frames selected as described above were subcloned into a T7 expression vector pXI and expressed using an in vitro the Escherichia coli transcription and cell free translation (IVTT) system (Rapid Translation System, Biotechrabbit). .. After expression the proteins were printed onto nitrocellulose-coated AVID slides (Grace Bio-Labs Inc.) using an Omni Grid Accent robotic microarray printer (Digilabs Inc.). ..

Article Title: Strong off-target antibody reactivity to malarial antigens induced by RTS,S/AS01E vaccination is associated with protection
Article Snippet: .. After expressing the proteins according to manufacturer instructions, translated proteins were printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs, Inc.) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc.). ..

Article Title: Characterization of SARS-CoV-2 antibodies in human milk from 21 women with confirmed COVID-19 infection
Article Snippet: .. Proteins expressed using an E. coli cell-free in vitro transcription and translation (IVTT) system, purified proteins and peptides were printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs, Inc., Bend, OR, USA) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc., Marlborough, MA, USA). .. Microarrays were probed with breast milk at a 1:15 dilution factor and antibody binding detected by incubation with Cy3 fluorochrome-conjugated goat anti-human IgA (Cat# 109-166-011, Jackson ImmunoResearch, West Grove, PA, USA).

Article Title: Mapping SARS-CoV-2 Antibody Epitopes in COVID-19 Patients with a Multi-Coronavirus Protein Microarray
Article Snippet: All these coronavirus proteins were produced in Escherichia coli except the SARS-CoV-2 and SARS-CoV S proteins, which were made in Sf9 insect cells, and the SARS-CoV-2 RBD, made in HEK-293 cells. .. Other proteins and protein fragments were expressed using an E. coli in vitro transcription and translation (IVTT) system (rapid translation system; Biotechrabbit, Berlin, Germany) and printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs, Inc., Bend, OR, USA) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc., Marlborough, MA, USA). .. Microarrays were probed with sera and antibody binding detected by incubation with fluorochrome-conjugated goat anti-human IgG, IgA, or IgM (Jackson ImmunoResearch, West Grove, PA, USA, or Bethyl Laboratories, Inc., Montgomery, TX, USA).

Article Title: The acquisition of humoral immune responses targeting Plasmodium falciparum sexual stages in controlled human malaria infections
Article Snippet: PCR amplification and cloning were successful for 568 unique genes (making up 943 distinct sequences) all of which were expressed in an in vitro transcription and translation (IVTT) system (5 Prime, Gaithersburg, MD, USA) according to manufacturer instructions, and as described previously ( , ). .. Arrays were printed onto 8-pad nitrocellulose-coated glass AVID slides (Grace Bio-Labs, Inc., Bend, OR, USA) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc., Marlborough, MA, USA). ..

Article Title: Diagnostic validation of novel Borrelia antigens discovered by whole-proteome microarray: Advancing early detection and test of cure for Lyme disease.
Article Snippet: The in vitro expressed proteins were printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs Inc., Bend, OR), which contained nitrocellulose ‘‘pads’’ per slide for replicate arrays. .. This was done using an Omni Grid Accent robotic microarray printer (Digilabs Inc., Hopkinton, MA). ..

In Vitro:

Article Title: Characterization of SARS-CoV-2 antibodies in human milk from 21 women with confirmed COVID-19 infection
Article Snippet: .. Proteins expressed using an E. coli cell-free in vitro transcription and translation (IVTT) system, purified proteins and peptides were printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs, Inc., Bend, OR, USA) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc., Marlborough, MA, USA). .. Microarrays were probed with breast milk at a 1:15 dilution factor and antibody binding detected by incubation with Cy3 fluorochrome-conjugated goat anti-human IgA (Cat# 109-166-011, Jackson ImmunoResearch, West Grove, PA, USA).

Article Title: Mapping SARS-CoV-2 Antibody Epitopes in COVID-19 Patients with a Multi-Coronavirus Protein Microarray
Article Snippet: All these coronavirus proteins were produced in Escherichia coli except the SARS-CoV-2 and SARS-CoV S proteins, which were made in Sf9 insect cells, and the SARS-CoV-2 RBD, made in HEK-293 cells. .. Other proteins and protein fragments were expressed using an E. coli in vitro transcription and translation (IVTT) system (rapid translation system; Biotechrabbit, Berlin, Germany) and printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs, Inc., Bend, OR, USA) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc., Marlborough, MA, USA). .. Microarrays were probed with sera and antibody binding detected by incubation with fluorochrome-conjugated goat anti-human IgG, IgA, or IgM (Jackson ImmunoResearch, West Grove, PA, USA, or Bethyl Laboratories, Inc., Montgomery, TX, USA).

Purification:

Article Title: Characterization of SARS-CoV-2 antibodies in human milk from 21 women with confirmed COVID-19 infection
Article Snippet: .. Proteins expressed using an E. coli cell-free in vitro transcription and translation (IVTT) system, purified proteins and peptides were printed onto nitrocellulose-coated glass AVID slides (Grace Bio-Labs, Inc., Bend, OR, USA) using an Omni Grid Accent robotic microarray printer (Digilabs, Inc., Marlborough, MA, USA). .. Microarrays were probed with breast milk at a 1:15 dilution factor and antibody binding detected by incubation with Cy3 fluorochrome-conjugated goat anti-human IgA (Cat# 109-166-011, Jackson ImmunoResearch, West Grove, PA, USA).



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Figure 2. (A) Layout of the biomarker <t>microarray</t> (BMA) slide, showing the arrangement of capture antibodies and controls within each well. (B) Illustration of the BMA slide design with 16 wells and its alignment within the slide holder for analysis. The numbers along the side correspond to the row numbers of each well, while the numbers along the bottom represent the column positions of the slide.
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Figure 2. (A) Layout of the biomarker microarray (BMA) slide, showing the arrangement of capture antibodies and controls within each well. (B) Illustration of the BMA slide design with 16 wells and its alignment within the slide holder for analysis. The numbers along the side correspond to the row numbers of each well, while the numbers along the bottom represent the column positions of the slide.

Journal: Micromachines

Article Title: Portable Fluorescence Microarray Reader-Enabled Biomarker Panel Detection System for Point-of-Care Diagnosis of Lupus Nephritis.

doi: 10.3390/mi16020156

Figure Lengend Snippet: Figure 2. (A) Layout of the biomarker microarray (BMA) slide, showing the arrangement of capture antibodies and controls within each well. (B) Illustration of the BMA slide design with 16 wells and its alignment within the slide holder for analysis. The numbers along the side correspond to the row numbers of each well, while the numbers along the bottom represent the column positions of the slide.

Article Snippet: The slides were prepared using a non-contact microarray printing robot (sciFLEXARRAYER S3; Scienion GmbH, Berlin, Germany), which printed capture antibodies for each biomarker in triplicate at a controlled drop volume of 450 ± 20 pL.

Techniques: Biomarker Discovery, Microarray