spotting solution for microarray printing  (Matsunami Glass)

Journal: BMC Genomics

Article Title: A highly sensitive and specific system for large-scale gene expression profiling

doi: 10.1186/1471-2164-9-9

Figure Lengend Snippet: Comparison of microarray results between duplicated samples . A . A scatter plot of the microarray intensities of a sample with 100 NCI/ADR-RES cells were plotted against those of a duplicated 100-cell sample, and B , a scatter plot in A after replacing the data of the latter 100-cell sample with data of a 10,000-cell sample from the same cell line. Microarray intensities shown in the figure are the results after baseline subtraction. Both plots are on the log scale.

Article Snippet: Slides were dried by centrifugation at 1,000 rpm for 5 min with a slide holder in a GS-6 Beckman centrifuge, and then were baked at 140°C in a vacuum oven for 4–5 hrs (Fisher Scientific Model 280A); (2) microarray preparation: each oligonucleotide probe was mixed with the Microarray Printing Solution (GenScript, Piscataway NJ) at a 1:5 ratio (v/v) to a final concentration of 50 μM in a well of a 384-well plate.

Techniques: Comparison, Microarray

Journal: BMC Genomics

Article Title: A highly sensitive and specific system for large-scale gene expression profiling

doi: 10.1186/1471-2164-9-9

Figure Lengend Snippet: Differential gene expression in the two cell lines, MCF-7 and NCI/ADR-RES . Upper , Microarray images from the two cell lines. Lower , confirmation of the gene expression profiling results by gel assay. Quantitative results are given in Table 3. M, MCF-7, and A, NCI/ADR-RES.

Article Snippet: Slides were dried by centrifugation at 1,000 rpm for 5 min with a slide holder in a GS-6 Beckman centrifuge, and then were baked at 140°C in a vacuum oven for 4–5 hrs (Fisher Scientific Model 280A); (2) microarray preparation: each oligonucleotide probe was mixed with the Microarray Printing Solution (GenScript, Piscataway NJ) at a 1:5 ratio (v/v) to a final concentration of 50 μM in a well of a 384-well plate.

Techniques: Gene Expression, Microarray

Journal: BMC Genomics

Article Title: A highly sensitive and specific system for large-scale gene expression profiling

doi: 10.1186/1471-2164-9-9

Figure Lengend Snippet: Comparison between the results from microarray and gel assay*

Article Snippet: Slides were dried by centrifugation at 1,000 rpm for 5 min with a slide holder in a GS-6 Beckman centrifuge, and then were baked at 140°C in a vacuum oven for 4–5 hrs (Fisher Scientific Model 280A); (2) microarray preparation: each oligonucleotide probe was mixed with the Microarray Printing Solution (GenScript, Piscataway NJ) at a 1:5 ratio (v/v) to a final concentration of 50 μM in a well of a 384-well plate.

Techniques: Comparison, Microarray