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microarray printer sciflexarrayer s12  (SCIENION)


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    Structured Review

    SCIENION microarray printer sciflexarrayer s12
    Microarray Printer Sciflexarrayer S12, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+printers/sciFLEXARRAYER+S12/pmc12888686-149-66-70
    Average 95 stars, based on 74 article reviews
    microarray printer sciflexarrayer s12 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study.
    Article Snippet: .. Characterization of surface markers on EVs with the EV Array To study the EV surface- and surface associated biomarkers on, plasma EVs were captured and visualized using the EV Array protocol outlined by Jørgensen et al., 2013 with a 54 EV marker antibody panel (table 1), which were diluted to 200 μg/mL in 50 mM trehalose in phosphate-buffered saline (PBS) and printed in triplicates on epoxysilane-coated slides (75.6 × 25 mm, Schott MINIFAB, Germany) using the microarray printer sciFLEXARRAYER S12 (Scienion AG, Berlin, Germany) with a PDC60. .. 50 mM trehalose in PBS was applied as a negative control and biotinylated goat anti-mouse IgG (Novus Biologicals, Centennial, CO, USA) with final concentrations of 5, 10, and 20 μg/mL were used as positive control.

    Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study
    Article Snippet: .. To study the EV surface- and surface associated biomarkers on, plasma EVs were captured and visualized using the EV Array protocol outlined by Jørgensen et al., 2013 with a 54 EV marker antibody panel (Table ), which were diluted to 200 μg/mL in 50 mM trehalose in phosphate-buffered saline (PBS) and printed in triplicates on epoxysilane-coated slides (75.6 × 25 mm, Schott MINIFAB, Germany) using the microarray printer sciFLEXARRAYER S12 (Scienion AG, Berlin, Germany) with a PDC60. .. 50 mM trehalose in PBS was applied as a negative control and biotinylated goat anti-mouse IgG (Novus Biologicals, Centennial, CO, USA) with final concentrations of 5, 10, and 20 μg/mL were used as positive control.

    Marker:

    Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study.
    Article Snippet: .. Characterization of surface markers on EVs with the EV Array To study the EV surface- and surface associated biomarkers on, plasma EVs were captured and visualized using the EV Array protocol outlined by Jørgensen et al., 2013 with a 54 EV marker antibody panel (table 1), which were diluted to 200 μg/mL in 50 mM trehalose in phosphate-buffered saline (PBS) and printed in triplicates on epoxysilane-coated slides (75.6 × 25 mm, Schott MINIFAB, Germany) using the microarray printer sciFLEXARRAYER S12 (Scienion AG, Berlin, Germany) with a PDC60. .. 50 mM trehalose in PBS was applied as a negative control and biotinylated goat anti-mouse IgG (Novus Biologicals, Centennial, CO, USA) with final concentrations of 5, 10, and 20 μg/mL were used as positive control.

    Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study
    Article Snippet: .. To study the EV surface- and surface associated biomarkers on, plasma EVs were captured and visualized using the EV Array protocol outlined by Jørgensen et al., 2013 with a 54 EV marker antibody panel (Table ), which were diluted to 200 μg/mL in 50 mM trehalose in phosphate-buffered saline (PBS) and printed in triplicates on epoxysilane-coated slides (75.6 × 25 mm, Schott MINIFAB, Germany) using the microarray printer sciFLEXARRAYER S12 (Scienion AG, Berlin, Germany) with a PDC60. .. 50 mM trehalose in PBS was applied as a negative control and biotinylated goat anti-mouse IgG (Novus Biologicals, Centennial, CO, USA) with final concentrations of 5, 10, and 20 μg/mL were used as positive control.

    Saline:

    Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study.
    Article Snippet: .. Characterization of surface markers on EVs with the EV Array To study the EV surface- and surface associated biomarkers on, plasma EVs were captured and visualized using the EV Array protocol outlined by Jørgensen et al., 2013 with a 54 EV marker antibody panel (table 1), which were diluted to 200 μg/mL in 50 mM trehalose in phosphate-buffered saline (PBS) and printed in triplicates on epoxysilane-coated slides (75.6 × 25 mm, Schott MINIFAB, Germany) using the microarray printer sciFLEXARRAYER S12 (Scienion AG, Berlin, Germany) with a PDC60. .. 50 mM trehalose in PBS was applied as a negative control and biotinylated goat anti-mouse IgG (Novus Biologicals, Centennial, CO, USA) with final concentrations of 5, 10, and 20 μg/mL were used as positive control.

    Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study
    Article Snippet: .. To study the EV surface- and surface associated biomarkers on, plasma EVs were captured and visualized using the EV Array protocol outlined by Jørgensen et al., 2013 with a 54 EV marker antibody panel (Table ), which were diluted to 200 μg/mL in 50 mM trehalose in phosphate-buffered saline (PBS) and printed in triplicates on epoxysilane-coated slides (75.6 × 25 mm, Schott MINIFAB, Germany) using the microarray printer sciFLEXARRAYER S12 (Scienion AG, Berlin, Germany) with a PDC60. .. 50 mM trehalose in PBS was applied as a negative control and biotinylated goat anti-mouse IgG (Novus Biologicals, Centennial, CO, USA) with final concentrations of 5, 10, and 20 μg/mL were used as positive control.

    Microarray:

    Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study.
    Article Snippet: .. Characterization of surface markers on EVs with the EV Array To study the EV surface- and surface associated biomarkers on, plasma EVs were captured and visualized using the EV Array protocol outlined by Jørgensen et al., 2013 with a 54 EV marker antibody panel (table 1), which were diluted to 200 μg/mL in 50 mM trehalose in phosphate-buffered saline (PBS) and printed in triplicates on epoxysilane-coated slides (75.6 × 25 mm, Schott MINIFAB, Germany) using the microarray printer sciFLEXARRAYER S12 (Scienion AG, Berlin, Germany) with a PDC60. .. 50 mM trehalose in PBS was applied as a negative control and biotinylated goat anti-mouse IgG (Novus Biologicals, Centennial, CO, USA) with final concentrations of 5, 10, and 20 μg/mL were used as positive control.

    Article Title: Direct Profiling of Extracellular Vesicle Surface Markers in Plasma: A Proof-of-Concept Study.
    Article Snippet: .. These antibodies (200 μg/mL) were printed in triplicates onto epoxysilane-coated slides (75.6 mm × 25 mm, Nexterion, Schott MINIFAB, DE) with the microarray printer sciFLEXARRAYER S12 installed with a size 60 piezo dispense capillary with type 3 coating (Scienion AG, DE). ..

    Article Title: Direct Profiling of Extracellular Vesicle Surface Markers in Plasma: A Proof‐of‐Concept Study
    Article Snippet: .. These antibodies (200 μg/mL) were printed in triplicates onto epoxysilane‐coated slides (75.6 mm × 25 mm, Nexterion, Schott MINIFAB, DE) with the microarray printer sciFLEXARRAYER S12 installed with a size 60 piezo dispense capillary with type 3 coating (Scienion AG, DE). ..

    Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study
    Article Snippet: .. To study the EV surface- and surface associated biomarkers on, plasma EVs were captured and visualized using the EV Array protocol outlined by Jørgensen et al., 2013 with a 54 EV marker antibody panel (Table ), which were diluted to 200 μg/mL in 50 mM trehalose in phosphate-buffered saline (PBS) and printed in triplicates on epoxysilane-coated slides (75.6 × 25 mm, Schott MINIFAB, Germany) using the microarray printer sciFLEXARRAYER S12 (Scienion AG, Berlin, Germany) with a PDC60. .. 50 mM trehalose in PBS was applied as a negative control and biotinylated goat anti-mouse IgG (Novus Biologicals, Centennial, CO, USA) with final concentrations of 5, 10, and 20 μg/mL were used as positive control.

    Concentration Assay:

    Article Title: Microarrayed Allergen Molecules Distinguish IgE Sensitisation to Blomia tropicalis and Dermatophagoides pteronyssinus.
    Article Snippet: The recombinant Dermatophagoides pteronyssinus allergens (Der p allergens: rDer p 4 (GenBank: AAD38942), rDer p 5 (GenBank: CAA35692), rDer p 7 (GenBank: AAA80264), rDer p 10 (GenBank: CAA75141), rDer p 18 (GenBank: AAY84563), rDer p 20 (GenBank: ACD50950), rDer p 21 (GenBank: ABC73706), rDer p 23 (GenBank: ACB46292) and rDer p 37 (GenBank: AVD73319)) were produced and characterised as described [26, 28, 37–43]. nDer p 1 and nDer p 2 were purchased from Citeq (Groningen, Netherlands) and Inbio (Charlottesville, VA), respectively. .. Allergens were dissolved in a concentration of 0.5–1 mg/mL in phosphate buffer (75 mM NaH2PO4, pH 8.4) and spotted in triplicates onto glass slides (Paul Marienfeld GmbH & Co. KG, Lauda- Königshofen, Germany) using a SciFlexArrayer S12 (Scienion AG, Berlin, Germany) [44]. ..



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    <t>Microarray</t> analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D
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    Image Search Results


    Comprehensive microarray polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.

    Journal: Plant Direct

    Article Title: Arabinokinase Limits the Flux of Arabinose Into Nucleotide Sugars to Prevent Toxicity

    doi: 10.1002/pld3.70094

    Figure Lengend Snippet: Comprehensive microarray polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.

    Article Snippet: Arrays were printed as distinct dots onto nitrocellulose membranes with an ArrayJet Sprint microarray printer (ArrayJet, Roslin, UK) and probed with ~40 cell wall probes, including antibodies and carbohydrate binding modules (Table ).

    Techniques: Microarray, Polymer, Residue, Isolation, Binding Assay

    Microarray analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

    Journal: Journal of Neuroinflammation

    Article Title: Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection

    doi: 10.1186/s12974-025-03482-8

    Figure Lengend Snippet: Microarray analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

    Article Snippet: Briefly, the microarray was prepared using a noncontact microarray printer (SciFLEXARRAYER S3; Scienion, Berlin, Germany).

    Techniques: Microarray, Expressing, Marker, Activation Assay, Software, Binding Assay