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BioRobotics Ltd microgrid ii microarraying robot
Microgrid Ii Microarraying Robot, supplied by BioRobotics Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+printer+biorobotics+microgrid+ii/microgrid+ii+microarray+printing+robot/pmc00497576-159-32-41
Average 90 stars, based on 1 article reviews
microgrid ii microarraying robot - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Use of DNA Microarrays for Rapid Genotyping of TEM Beta-Lactamases That Confer Resistance
Article Snippet: For covalent immobilization, the oligonucleotide array was incubated at 120°C for 30 min in a drying compartment (Memmert, Schwabach, Germany).

Concentration Assay:

Article Title: Lectin microarrays differentiate carcinoma cells from reactive mesothelial cells in pleural effusions
Article Snippet: .. Lectins (Vector Laboratories, Inc., Burlingame, CA, USA) (Table ) were dissolved in spotting solution at a concentration of 1 mg/mL (SNA, MAL-I and MAL-II) or 0.5 mg/mL (the other lectins), and spotted in duplicate (Fig. ) onto a 3-glycidoxypropyltrimethoxysilane glass slide using a microarray printing robot (MicroGridII, BioRobotics Ltd., Cambridge, UK). ..

Article Title: Use of DNA Microarrays for Rapid Genotyping of TEM Beta-Lactamases That Confer Resistance
Article Snippet: .. The probes were dissolved in spotting buffer (160 mM Na2SO4, 130 mM Na2HPO4) to a final concentration of 20 M and spotted with a MicroGrid II microarraying robot by using MicroSpot 2500 pins (BioRobotics, Cambridge, United Kingdom) on epoxy-coated glass slides (Elipsa AG, Berlin, Germany). .. For covalent immobilization, the oligonucleotide array was incubated at 120°C for 30 min in a drying compartment (Memmert, Schwabach, Germany).

Microarray:

Article Title: Lectin microarrays differentiate carcinoma cells from reactive mesothelial cells in pleural effusions
Article Snippet: .. Lectins (Vector Laboratories, Inc., Burlingame, CA, USA) (Table ) were dissolved in spotting solution at a concentration of 1 mg/mL (SNA, MAL-I and MAL-II) or 0.5 mg/mL (the other lectins), and spotted in duplicate (Fig. ) onto a 3-glycidoxypropyltrimethoxysilane glass slide using a microarray printing robot (MicroGridII, BioRobotics Ltd., Cambridge, UK). ..

Article Title: Gene expression profile analysis of mouse colon embryonic development.
Article Snippet: Young-Kyu Park, Jeffrey L. Franklin, Stephen H. Settle, Shawn E. Levy, Eunkyung Chung, Loice H. Jeyakumar, Yu Shyr, Mary Kay Washington, Robert H. Whitehead, Bruce J. Aronow, and Robert J. Coffey* Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee Department of Biomedical Informatics, Vanderbilt University School of Medicine, Nashville, Tennessee Department of Preventive Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee Division of Developmental Biology and Division of Pediatric Informatics, Children’s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio Department of Veterans Affairs Medical Center, Nashville, Tennessee

Article Title: Biomarkers quantification with antibody arrays in cancer early detection.
Article Snippet: Cancer is the second most common cause of death in the United States, accounting for nearly one of every four deaths, exceeded only by heart disease.. Cancer is more treatable when diagnosed at the early stage, and this is particularly relevant for cancers of the breast, cervix, mouth, larynx, colon and rectum, and skin.. Moreover, cancers of the cervix, colon, and rectum can be prevented by removal of precancerous tissue.

Article Title: Virulence genes of Porphyromonas gingivalis W83 in chronic periodontitis.
Article Snippet: Objective.. To identify virulence genes found in highly virulent strains of Porphyromonas gingivalis (P. gingivalis) among Chinese patients with chronic periodontitis and to evaluate the association of these virulence genes with clinical parameters and with periodontal tissue destruction.. Material and methods.

Article Title: Identification of Genes, including the Gene Encoding p27Kip1, Regulated by Serine 276 Phosphorylation of the p65 Subunit of NF-κB
Article Snippet: .. The PCR products were then transposed to 384 well plates and robotically arrayed using a BioRobotics MicroGrid II microarray printing robot (Apogent Discoveries, Hudson, NH) onto polylysine-coated glass slides (Cel Associates, Pearland, TX). ..

Polymerase Chain Reaction:

Article Title: Gene expression profile analysis of mouse colon embryonic development.
Article Snippet: Young-Kyu Park, Jeffrey L. Franklin, Stephen H. Settle, Shawn E. Levy, Eunkyung Chung, Loice H. Jeyakumar, Yu Shyr, Mary Kay Washington, Robert H. Whitehead, Bruce J. Aronow, and Robert J. Coffey* Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee Department of Biomedical Informatics, Vanderbilt University School of Medicine, Nashville, Tennessee Department of Preventive Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee Division of Developmental Biology and Division of Pediatric Informatics, Children’s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio Department of Veterans Affairs Medical Center, Nashville, Tennessee

Article Title: Identification of Genes, including the Gene Encoding p27Kip1, Regulated by Serine 276 Phosphorylation of the p65 Subunit of NF-κB
Article Snippet: .. The PCR products were then transposed to 384 well plates and robotically arrayed using a BioRobotics MicroGrid II microarray printing robot (Apogent Discoveries, Hudson, NH) onto polylysine-coated glass slides (Cel Associates, Pearland, TX). ..

Biomarker Discovery:

Article Title: Biomarkers quantification with antibody arrays in cancer early detection.
Article Snippet: Cancer is the second most common cause of death in the United States, accounting for nearly one of every four deaths, exceeded only by heart disease.. Cancer is more treatable when diagnosed at the early stage, and this is particularly relevant for cancers of the breast, cervix, mouth, larynx, colon and rectum, and skin.. Moreover, cancers of the cervix, colon, and rectum can be prevented by removal of precancerous tissue.



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BioRobotics Ltd microarray printer biorobotics microgrid ii
Testing the limits of NRF2 binding. Systematic multivariate ARE results by protein binding <t>microarray</t> for NRF2-MAFG heterodimer are shown. Tolerance for sequence variation was studied for positions 1–3 (white), 6–8 (grey) and 9–11 (dark grey) ( A ) and positions 1, 5 and 11 (seed sequences are shown in grey) ( B ). Results are depicted as measured binding relative to NQO1.ARE binding (black) (mean ± S.E.M., n = 45).
Microarray Printer Biorobotics Microgrid Ii, supplied by BioRobotics Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+printer+biorobotics+microgrid+ii/microgrid+ii+microarray+robot/pmc04770247-73-24-26
Average 90 stars, based on 1 article reviews
microarray printer biorobotics microgrid ii - by Bioz Stars, 2026-10
90/100 stars
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Testing the limits of NRF2 binding. Systematic multivariate ARE results by protein binding microarray for NRF2-MAFG heterodimer are shown. Tolerance for sequence variation was studied for positions 1–3 (white), 6–8 (grey) and 9–11 (dark grey) ( A ) and positions 1, 5 and 11 (seed sequences are shown in grey) ( B ). Results are depicted as measured binding relative to NQO1.ARE binding (black) (mean ± S.E.M., n = 45).

Journal: Nucleic Acids Research

Article Title: The Effects of Sequence Variation on Genome-wide NRF2 Binding—New Target Genes and Regulatory SNPs

doi: 10.1093/nar/gkw052

Figure Lengend Snippet: Testing the limits of NRF2 binding. Systematic multivariate ARE results by protein binding microarray for NRF2-MAFG heterodimer are shown. Tolerance for sequence variation was studied for positions 1–3 (white), 6–8 (grey) and 9–11 (dark grey) ( A ) and positions 1, 5 and 11 (seed sequences are shown in grey) ( B ). Results are depicted as measured binding relative to NQO1.ARE binding (black) (mean ± S.E.M., n = 45).

Article Snippet: The dilutions were dispensed in a 384-well plate (polypropylene plate No 267462, Nunc, N.Y, USA) and printed onto the avidin-coated glass slides with a microarray printer (BioRobotics MicroGrid II, BioRobotics Ltd, Cambridge, UK).

Techniques: Binding Assay, Protein Binding, Microarray, Sequencing

A SNP in FTL promoter has drastic effects of NRF2 binding and transcriptional activation. ( A ) A promoter analysis of the FTL gene at chr19 showing the location of experimentally verified NRF2 binding ARE together with dbSNP (v138) and ENCODE ChIP-seq data. ChIP-seq track displays combined MAFF and MAFK binding signals in H1-hESC (MAFK), K562 (MAFF, MAFK), HeLa-S3 (MAFK), HepG (MAFF, MAFK) and IMR90 (MAFK) cell lines. ( B ) Detailed view showing FTL ARE sequence and the SNP (rs113067944, A→C) position. ( C ) Protein binding microarray results for FTL.ARE.A and the SNP bearing FTL.ARE.C. Results are calculated as measured binding relative to NQO1.ARE binding (mean ± S.E.M, n = 39.). Scramble oligonucleotides served as negative control. ( D ) HEK-293T cells were transfected with NQO1-ARE and FTL-ARE bearing either allele A or allele C with and without NRF2 -expressing plasmids. Twenty-four h after transfection cells were treated with NRF2 inducer (L-SFN) for 16 h followed by luciferase activity measurements. An empty pGL3 promoter vector served as control and activities were normalized to β-galactosidase activity. Results are shown relative to control (mean± S.E.M, n = 4).

Journal: Nucleic Acids Research

Article Title: The Effects of Sequence Variation on Genome-wide NRF2 Binding—New Target Genes and Regulatory SNPs

doi: 10.1093/nar/gkw052

Figure Lengend Snippet: A SNP in FTL promoter has drastic effects of NRF2 binding and transcriptional activation. ( A ) A promoter analysis of the FTL gene at chr19 showing the location of experimentally verified NRF2 binding ARE together with dbSNP (v138) and ENCODE ChIP-seq data. ChIP-seq track displays combined MAFF and MAFK binding signals in H1-hESC (MAFK), K562 (MAFF, MAFK), HeLa-S3 (MAFK), HepG (MAFF, MAFK) and IMR90 (MAFK) cell lines. ( B ) Detailed view showing FTL ARE sequence and the SNP (rs113067944, A→C) position. ( C ) Protein binding microarray results for FTL.ARE.A and the SNP bearing FTL.ARE.C. Results are calculated as measured binding relative to NQO1.ARE binding (mean ± S.E.M, n = 39.). Scramble oligonucleotides served as negative control. ( D ) HEK-293T cells were transfected with NQO1-ARE and FTL-ARE bearing either allele A or allele C with and without NRF2 -expressing plasmids. Twenty-four h after transfection cells were treated with NRF2 inducer (L-SFN) for 16 h followed by luciferase activity measurements. An empty pGL3 promoter vector served as control and activities were normalized to β-galactosidase activity. Results are shown relative to control (mean± S.E.M, n = 4).

Article Snippet: The dilutions were dispensed in a 384-well plate (polypropylene plate No 267462, Nunc, N.Y, USA) and printed onto the avidin-coated glass slides with a microarray printer (BioRobotics MicroGrid II, BioRobotics Ltd, Cambridge, UK).

Techniques: Binding Assay, Activation Assay, ChIP-sequencing, Sequencing, Protein Binding, Microarray, Negative Control, Transfection, Expressing, Luciferase, Activity Assay, Plasmid Preparation, Control