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WY14,643 treatment of PBMCs induced a strong response in <t>microarray</t> gene expression but caused only a minor effect on DNA methylation. a In response to WY14,643-treatment 2907 probes displayed significant ( p < 0.01) differential methylation 4 of which containing over 5 % change between control and Wy-treated samples. These probes were not associated to the 561 genes displaying significant ( p < 0.01) differential expression. b Strong induction of FAPB4 expression upon WY treatment. c DNA methylation of the six probes representing the highest PPARα-response gene FABP4 on the 450K array did not show a change in DNA methylation upon WY14,643 treatment
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WY14,643 treatment of PBMCs induced a strong response in microarray gene expression but caused only a minor effect on DNA methylation. a In response to WY14,643-treatment 2907 probes displayed significant ( p < 0.01) differential methylation 4 of which containing over 5 % change between control and Wy-treated samples. These probes were not associated to the 561 genes displaying significant ( p < 0.01) differential expression. b Strong induction of FAPB4 expression upon WY treatment. c DNA methylation of the six probes representing the highest PPARα-response gene FABP4 on the 450K array did not show a change in DNA methylation upon WY14,643 treatment

Journal: Age

Article Title: Genome-wide age-related changes in DNA methylation and gene expression in human PBMCs

doi: 10.1007/s11357-014-9648-x

Figure Lengend Snippet: WY14,643 treatment of PBMCs induced a strong response in microarray gene expression but caused only a minor effect on DNA methylation. a In response to WY14,643-treatment 2907 probes displayed significant ( p < 0.01) differential methylation 4 of which containing over 5 % change between control and Wy-treated samples. These probes were not associated to the 561 genes displaying significant ( p < 0.01) differential expression. b Strong induction of FAPB4 expression upon WY treatment. c DNA methylation of the six probes representing the highest PPARα-response gene FABP4 on the 450K array did not show a change in DNA methylation upon WY14,643 treatment

Article Snippet: A microarray platform (Infinium HumanMethylation450 BeadChips ; Infinium Inc., San Diego, CA, USA) was used, which was processed by the UCL Genomics Core Facility in accordance with the manufacturer’s recommendation.

Techniques: Microarray, Gene Expression, DNA Methylation Assay, Methylation, Control, Quantitative Proteomics, Expressing