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carbon source microarray plates  (Biolog Inc)

 
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    Biolog Inc carbon source microarray plates
    Carbon Source Microarray Plates, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+plates/phenotype+microarray+plates+for+carbon+catabolism/pm40497710-51-4-3
    Average 90 stars, based on 1 article reviews
    carbon source microarray plates - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Bacteria:

    Article Title: A host-pathogen metabolic synchrony that facilitates disease tolerance.
    Article Snippet: .. Carbon source utilization assay For the Carbon Source Phenotype MicroarrayTM (Biolog), a stock solution of 2 × 107 bacteria/mL of overnight bacteria grown in LB was washed andprepared in 1X IF-Oabuffer (Biolog#72268) supplemented with 1X Redox DyeMix A (Biolog #74221). .. Subsequently, 100μL of this stock solution (delivering 2 × 106 bacteria) was added to each well of a PM1 MicroplateTM (Biolog #12111) and the plate was incubated at 37 °C for 24 hr.

    Article Title: Regulation of airway fumarate by host and pathogen promotes S. aureus pneumonia
    Article Snippet: .. For Carbon Source Phenotype MicroarrayTM (Biolog) assays, a stock solution of 2 × 10 7 bacteria/ml was prepared in 1X IF-Oa buffer (Biolog, 72268) supplemented with 1X Redox Dye Mix A (Biolog, 74221). .. 100 μ l of this stock solution (delivering 2 × 10 6 bacteria) was added to each well of a PM1 MicroplateTM (Biolog, 12111) and the plate was incubated at 37°C overnight with shaking.

    Article Title: A host-pathogen metabolic synchrony that facilitates disease tolerance
    Article Snippet: .. For the Carbon Source Phenotype Microarray TM (Biolog), a stock solution of 2 × 10 7 bacteria/mL of overnight bacteria grown in LB was washed and prepared in 1X IF-Oa buffer (Biolog #72268) supplemented with 1X Redox Dye Mix A (Biolog #74221). .. Subsequently, 100 μL of this stock solution (delivering 2 × 10 6 bacteria) was added to each well of a PM1 Microplate TM (Biolog #12111) and the plate was incubated at 37 °C for 24 hr.

    Microarray:

    Article Title: Control of citrate utilization by Candida albicans Adr1.
    Article Snippet: .. Growth tests with Biolog carbon source microarray plates (20) indicated that the triple mutant was defective in utilizing citrate, glutamate, and malate. ..

    Article Title: Mrs4 loss of function in fungi during adaptation to the cystic fibrosis lung.
    Article Snippet: .. Biolog carbon utilization phenotype microarray plates. ..

    Article Title: Mrs4 loss of function in fungi during adaptation to the cystic fibrosis lung
    Article Snippet: .. To further assess metabolic differences associated with Mrs4 LOF, we analyzed growth of B_L01 parent and mrs4 Δ:: MRS4 REF on different sole carbon sources using the Biolog TM carbon utilization phenotype microarray plates. ..

    Article Title: A host-pathogen metabolic synchrony that facilitates disease tolerance
    Article Snippet: .. For the Carbon Source Phenotype Microarray TM (Biolog), a stock solution of 2 × 10 7 bacteria/mL of overnight bacteria grown in LB was washed and prepared in 1X IF-Oa buffer (Biolog #72268) supplemented with 1X Redox Dye Mix A (Biolog #74221). .. Subsequently, 100 μL of this stock solution (delivering 2 × 10 6 bacteria) was added to each well of a PM1 Microplate TM (Biolog #12111) and the plate was incubated at 37 °C for 24 hr.

    Article Title: Mrs4 loss of function in fungi during adaptation to the cystic fibrosis lung.
    Article Snippet: .. To further assess metabolic differences associated with Mrs4 LOF, we analyzed growth of B_L01 parent and mrs4∆::MRS4REF on different sole carbon sources using the Biolog carbon utilization phenotype microarray plates. ..

    Mutagenesis:

    Article Title: Control of citrate utilization by Candida albicans Adr1.
    Article Snippet: .. Growth tests with Biolog carbon source microarray plates (20) indicated that the triple mutant was defective in utilizing citrate, glutamate, and malate. ..



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    SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype <t>Microarray</t> PM13.
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    Image Search Results


    SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.

    Journal: mBio

    Article Title: Physiological roles of an Acinetobacter -specific σ factor

    doi: 10.1128/mbio.00968-25

    Figure Lengend Snippet: SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.

    Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

    Techniques: RNA Sequencing, Over Expression, Plasmid Preparation, Control, Knockdown, Two Tailed Test, Activity Assay, Fluorescence, Microarray