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hybridization solution  (Cytiva Europe)


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    Structured Review

    Cytiva Europe hybridization solution
    Hybridization Solution, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+solution+version+2/Microarray+Hybridization+Solution+v2%2E0/pmc09317141-86-77-91
    Average 93 stars, based on 116 article reviews
    hybridization solution - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Comprehensive analysis of peripheral blood non-coding RNAs identifies a diagnostic panel for fungal infection after transplantation
    Article Snippet: .. 1ul of the cDNA is mixed with 5ul 10X of blocking reagent and 1ul 25X of separation buffer at 60°C for 30 minutes, and then be diluted by 25ul 2X of GE hybridization solution. .. It was further transferred into Mouse LncRNA Array (Arraystar, USA, v2.0) and incubated in an Agilent hybridization oven at 65°C for 17 hours.

    Hybridization:

    Article Title: Comprehensive analysis of peripheral blood non-coding RNAs identifies a diagnostic panel for fungal infection after transplantation
    Article Snippet: .. 1ul of the cDNA is mixed with 5ul 10X of blocking reagent and 1ul 25X of separation buffer at 60°C for 30 minutes, and then be diluted by 25ul 2X of GE hybridization solution. .. It was further transferred into Mouse LncRNA Array (Arraystar, USA, v2.0) and incubated in an Agilent hybridization oven at 65°C for 17 hours.

    Article Title: A chemical defense deters cannibalism in migratory locusts
    Article Snippet: .. Subsequently, each slide was covered with 130 μl hybridization solution [50% formamide, 25% H2O, 25% Microarray Hybridization Solution Version 2.0 (GE Healthcare, Freiburg, Germany)] containing either the labeled antisense or sense RNA probe. ..

    Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells
    Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of hybridization solution was dispensed into the gasket slide and assembled to the lncRNA expression microarray slide. .. Slides were incubated for 17 h at 65°C in an Agilent Hybridization Oven.

    Article Title: Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription
    Article Snippet: .. From this, 4 μg of DNA was labeled with fluorescent Cy5 dye and used for the microarray hybridization (GE Amersham, USA). ..

    Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens
    Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Microarray Hybridization Solution Version 2 (Amersham Biosciences) and formamide (Sigma Chemical Co, St Louis, MO, USA) to a final concentration of 50%. ..

    Article Title: Long noncoding intronic RNAs are differentially expressed in primary and metastatic pancreatic cancer
    Article Snippet: .. For each sample, cRNA targets were ressuspended in a final volume of 200 μl of 1× Microarray Hybridization Solution v.2.0 (GE Healthcare) containing 25% formamide, denaturated at 92°C for 2 minutes and incubated with microarrays at 42°C for 16 hours using an automated slide processor (GE Healthcare). .. Following sequential washes in 1× SSC; 0.2% SDS, 0.1× SSC; 0.2% SDS and 0.1× SSC; 0.2% SDS, microarray slides were scanned immediately in a Generation III Microarray Systems Scanner (Molecular Dynamics/GE Healthcare).

    Article Title: A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera.
    Article Snippet: .. Each slide with tissue sections was covered with 130 μL hybridization solution 1 [50% formamide, 25% H2O, 25% Microarray hybridization solution version 2.0 (GE Healthcare, Freiburg, Germany)] supplemented with the labeled riboprobe. ..

    Article Title: The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria
    Article Snippet: .. After sectioning, the samples were treated for 20 min at 4 °C with 4% paraformaldehyde dissolved in PBS, washed in PBS for 5 min, and then incubated in 0.2 M HCl for 10 min. After washing the samples two times for 2 min in PBS, the slides were transferred into pre-hybridization solution (5× SSC (0.75 M NaCl, 0.075 M sodium citrate, pH 7.0) and 50% formamide) for 10 min. Next, each slide was covered with 130 μL hybridization solution (50% formamide, 25% H 2 O, 25% Microarray Hybridization Solution Version 2.0 (GE Healthcare, Freiburg, Germany)) containing either the ORCO or the SNMP1 antisense riboprobe. ..

    Microarray:

    Article Title: A chemical defense deters cannibalism in migratory locusts
    Article Snippet: .. Subsequently, each slide was covered with 130 μl hybridization solution [50% formamide, 25% H2O, 25% Microarray Hybridization Solution Version 2.0 (GE Healthcare, Freiburg, Germany)] containing either the labeled antisense or sense RNA probe. ..

    Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells
    Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of hybridization solution was dispensed into the gasket slide and assembled to the lncRNA expression microarray slide. .. Slides were incubated for 17 h at 65°C in an Agilent Hybridization Oven.

    Article Title: Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription
    Article Snippet: .. From this, 4 μg of DNA was labeled with fluorescent Cy5 dye and used for the microarray hybridization (GE Amersham, USA). ..

    Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens
    Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Microarray Hybridization Solution Version 2 (Amersham Biosciences) and formamide (Sigma Chemical Co, St Louis, MO, USA) to a final concentration of 50%. ..

    Article Title: Long noncoding intronic RNAs are differentially expressed in primary and metastatic pancreatic cancer
    Article Snippet: .. For each sample, cRNA targets were ressuspended in a final volume of 200 μl of 1× Microarray Hybridization Solution v.2.0 (GE Healthcare) containing 25% formamide, denaturated at 92°C for 2 minutes and incubated with microarrays at 42°C for 16 hours using an automated slide processor (GE Healthcare). .. Following sequential washes in 1× SSC; 0.2% SDS, 0.1× SSC; 0.2% SDS and 0.1× SSC; 0.2% SDS, microarray slides were scanned immediately in a Generation III Microarray Systems Scanner (Molecular Dynamics/GE Healthcare).

    Article Title: A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera.
    Article Snippet: .. Each slide with tissue sections was covered with 130 μL hybridization solution 1 [50% formamide, 25% H2O, 25% Microarray hybridization solution version 2.0 (GE Healthcare, Freiburg, Germany)] supplemented with the labeled riboprobe. ..

    Article Title: The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria
    Article Snippet: .. After sectioning, the samples were treated for 20 min at 4 °C with 4% paraformaldehyde dissolved in PBS, washed in PBS for 5 min, and then incubated in 0.2 M HCl for 10 min. After washing the samples two times for 2 min in PBS, the slides were transferred into pre-hybridization solution (5× SSC (0.75 M NaCl, 0.075 M sodium citrate, pH 7.0) and 50% formamide) for 10 min. Next, each slide was covered with 130 μL hybridization solution (50% formamide, 25% H 2 O, 25% Microarray Hybridization Solution Version 2.0 (GE Healthcare, Freiburg, Germany)) containing either the ORCO or the SNMP1 antisense riboprobe. ..

    Labeling:

    Article Title: A chemical defense deters cannibalism in migratory locusts
    Article Snippet: .. Subsequently, each slide was covered with 130 μl hybridization solution [50% formamide, 25% H2O, 25% Microarray Hybridization Solution Version 2.0 (GE Healthcare, Freiburg, Germany)] containing either the labeled antisense or sense RNA probe. ..

    Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells
    Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of hybridization solution was dispensed into the gasket slide and assembled to the lncRNA expression microarray slide. .. Slides were incubated for 17 h at 65°C in an Agilent Hybridization Oven.

    Article Title: Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription
    Article Snippet: .. From this, 4 μg of DNA was labeled with fluorescent Cy5 dye and used for the microarray hybridization (GE Amersham, USA). ..

    Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens
    Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Microarray Hybridization Solution Version 2 (Amersham Biosciences) and formamide (Sigma Chemical Co, St Louis, MO, USA) to a final concentration of 50%. ..

    Article Title: A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera.
    Article Snippet: .. Each slide with tissue sections was covered with 130 μL hybridization solution 1 [50% formamide, 25% H2O, 25% Microarray hybridization solution version 2.0 (GE Healthcare, Freiburg, Germany)] supplemented with the labeled riboprobe. ..

    Expressing:

    Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells
    Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of hybridization solution was dispensed into the gasket slide and assembled to the lncRNA expression microarray slide. .. Slides were incubated for 17 h at 65°C in an Agilent Hybridization Oven.

    Concentration Assay:

    Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens
    Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Microarray Hybridization Solution Version 2 (Amersham Biosciences) and formamide (Sigma Chemical Co, St Louis, MO, USA) to a final concentration of 50%. ..

    Incubation:

    Article Title: Long noncoding intronic RNAs are differentially expressed in primary and metastatic pancreatic cancer
    Article Snippet: .. For each sample, cRNA targets were ressuspended in a final volume of 200 μl of 1× Microarray Hybridization Solution v.2.0 (GE Healthcare) containing 25% formamide, denaturated at 92°C for 2 minutes and incubated with microarrays at 42°C for 16 hours using an automated slide processor (GE Healthcare). .. Following sequential washes in 1× SSC; 0.2% SDS, 0.1× SSC; 0.2% SDS and 0.1× SSC; 0.2% SDS, microarray slides were scanned immediately in a Generation III Microarray Systems Scanner (Molecular Dynamics/GE Healthcare).

    Article Title: The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria
    Article Snippet: .. After sectioning, the samples were treated for 20 min at 4 °C with 4% paraformaldehyde dissolved in PBS, washed in PBS for 5 min, and then incubated in 0.2 M HCl for 10 min. After washing the samples two times for 2 min in PBS, the slides were transferred into pre-hybridization solution (5× SSC (0.75 M NaCl, 0.075 M sodium citrate, pH 7.0) and 50% formamide) for 10 min. Next, each slide was covered with 130 μL hybridization solution (50% formamide, 25% H 2 O, 25% Microarray Hybridization Solution Version 2.0 (GE Healthcare, Freiburg, Germany)) containing either the ORCO or the SNMP1 antisense riboprobe. ..



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