tianamp genomic dna kit (tiangen biotech co)

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tiangen biotech co tianamp genomic dna kit

a Comparison of Etv5 expression levels between mESC lines and somatic cell lines. The relative expression was based on the microarray data from BioGPS database. b The interactions between pluripotency relevant regulators and Etv5 . ChIP-seq and ChIP-chip data with Etv5 as target were extracted from ESCAPE database and used for drawing these interactions. c Growth curve of J1 mESCs stably infected with shCtrl and Etv5 shRNA (shEtv5-7). d RT-qPCR analysis of Etv5 and Tet2 in mESCs stably infected with shCtrl, Etv5 shRNA (shEtv5-7), and shEtv5-7 plus lentiviral Etv5 . Data are shown as mean ± SD ( n = 3). * P < 0.05, *** P < 0.001. Two-way ANOVA with Sidak’s multiple comparisons test was used for c . One-way ANOVA with Dunnett’s multiple comparisons test for d . e Western blotting of TET2 in mESCs stably infected with shCtrl, shEtv5-7, and shEtv5-7 + Etv5 . GAPDH was used as internal control. The relative quantification is also shown. f Dot blot of global 5hmC in mESCs stably infected with shCtrl, shEtv5-7, and shEtv5-7 plus lentiviral Etv5 . The blotting result of serially diluted <t>genomic</t> <t>DNA</t> (100-3.125 ng) was shown (left panel). The same membrane stained with methylene blue as DNA loading control was also presented (right panel)

Tianamp Genomic Dna Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 98/100, based on 657 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridization+dna+microarray+analyses/TIANamp+Genomic+DNA+Kit/pmc05833841-264-12-16
Average 98 stars, based on 657 article reviews

tianamp genomic dna kit - by Bioz Stars, 2026-10

98/100 stars

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1) Product Images from "The oncogene Etv5 promotes MET in somatic reprogramming and orchestrates epiblast/primitive endoderm specification during mESCs differentiation"

Article Title: The oncogene Etv5 promotes MET in somatic reprogramming and orchestrates epiblast/primitive endoderm specification during mESCs differentiation

Journal: Cell Death & Disease

doi: 10.1038/s41419-018-0335-1

Figure Legend Snippet: a Comparison of Etv5 expression levels between mESC lines and somatic cell lines. The relative expression was based on the microarray data from BioGPS database. b The interactions between pluripotency relevant regulators and Etv5 . ChIP-seq and ChIP-chip data with Etv5 as target were extracted from ESCAPE database and used for drawing these interactions. c Growth curve of J1 mESCs stably infected with shCtrl and Etv5 shRNA (shEtv5-7). d RT-qPCR analysis of Etv5 and Tet2 in mESCs stably infected with shCtrl, Etv5 shRNA (shEtv5-7), and shEtv5-7 plus lentiviral Etv5 . Data are shown as mean ± SD ( n = 3). * P < 0.05, *** P < 0.001. Two-way ANOVA with Sidak’s multiple comparisons test was used for c . One-way ANOVA with Dunnett’s multiple comparisons test for d . e Western blotting of TET2 in mESCs stably infected with shCtrl, shEtv5-7, and shEtv5-7 + Etv5 . GAPDH was used as internal control. The relative quantification is also shown. f Dot blot of global 5hmC in mESCs stably infected with shCtrl, shEtv5-7, and shEtv5-7 plus lentiviral Etv5 . The blotting result of serially diluted genomic DNA (100-3.125 ng) was shown (left panel). The same membrane stained with methylene blue as DNA loading control was also presented (right panel)

Techniques Used: Comparison, Expressing, Microarray, ChIP-sequencing, ChIP-chip, Stable Transfection, Infection, shRNA, Quantitative RT-PCR, Western Blot, Control, Quantitative Proteomics, Dot Blot, Membrane, Staining

Real-time Polymerase Chain Reaction:

Article Title: The hypothalamic orexin–medial prefrontal cortex pathway modulates susceptibility to depression in the learned helplessness paradigm in male mice
Article Snippet: Finally, coverslips were mounted using neutral balsam 212 (Cat# 8590, Solarbio, Beijing, China). .. 213 Quantitative real-time PCR 214 Total RNA was extracted from fresh hypothalamic tissue using an RNA kit (Cat# DP304-02, 215 TIANGEN, Beijing, China). .. The concentration and purity of the RNA were assessed with a 216 NanoDrop 2000C (Thermo Fisher Scientific, USA).

Tissue:

DNA Extraction:

Article Title: Genetic Association Analysis of Skin Traits and the TAF11 Gene in Shenxian Pigs.
Article Snippet: .. DNA was extracted from ear tissue samples of 50 pigs using the Tiangen Fast DNA Extraction and Detection Kit (DP304-02, Tiangen Biotech Co., Ltd., Beijing, China) according to the manufacturer’s instructions. ..

Article Title: The EHMT2-MBLAC2 axis suppresses ribosomal DNA transcription in response to nucleolar DNA damage
Article Snippet: The 4D-FastDIA proteomic analysis was performed by PTM-Biolabs (Hangzhou, China). .. Genomic DNA extraction was performed with TIANamp Genomic DNA Kit (TIANGEN, DP304-02) following the manufacturer’s recommendation. ..

Article Title: Mn3O4 nanozymes increased cotton salt tolerance by recruiting S-adenosylmethionine synthase to reduce DNA methylation level
Article Snippet: .. TIANamp Blood Kit (Tiangen Biotech, cat. DP304–02) was used for DNA extraction. ..

Article Title: Combining molecular simulations and bitter receptor modelling reveals the bitter-taste mechanism of walnut protease hydrolysates.
Article Snippet: .. About 24 h post-transfection, the cells were harvested, and genomic DNA was extracted using the Cell Genomic DNA Extraction Kit (Tiangen, Cat. No. DP304-02). ..

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