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qiaamp dna mini stool kit  (Qiagen)


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    Structured Review

    Qiagen qiaamp dna mini stool kit
    Qiaamp Dna Mini Stool Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 56073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+dna+microarray+analyses/QIAamp+DNA+Mini+Kit/pm23319531-57-20-25
    Average 99 stars, based on 56073 article reviews
    qiaamp dna mini stool kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Small molecules rescue pathogenic POLGA aggregation and restore its functions in a mouse model of PEO.
    Article Snippet: Polyclonal Antibody, Rabbit polyclonal, living colours antibody (used Takara Cat# 632460 © The Author(s) EMBO Molecular Medicine 13 Reagent/resource Reference or source Identifier or Catalogue Number for YFP) (used for WB, IP) Tom20 (D8T4N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 42406 OPA1 (D6U6N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 80471 DRP1 (D6C7), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 8570 Phospho-DRP1 (Ser616) (D9A1), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 4494 Phospho-DRP1 (Ser637) (D3A4), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 6319 MFF (E5W4M), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 84580 MFN-1 (D6E2S), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 14739 MFN2 (D1E9), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 11925 Anti-Laminin, Rabbit antibody (used for IF) Sigma-Aldrich Cat# L9393 Anti-ATG12 antibody, Rabbit Polyclonal (used for WB) Abcam Cat# ab155589 Anti-Cytochrome C antibody, Mouse monoclonal (used for IF) Novus Biologicals Cat# NB100-56503 COX IV antibody, Rabbit antibody (used for IF) Cell Signalling Technology Cat# 4844 Myc-Tag (71D10), Rabbit monoclonal antibody (used for WB) Cell Signalling Technology Cat# 2278 Anti-His, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 8036 Anti- β actin, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 47778 Anti-GST, Rabbit antibody (used for WB) Thermo Fisher Scientific Cat# 71-7500 DNA Polymerase gamma Antibody; Rabbit Polyclonal (used for IF) Thermo Fisher Scientific Cat# BS-13017R Anti-RECQL4 (used for WB, IP) Santa Cruz Biotechnology Cat# sc-366840 Oligonucleotides and other sequence-based reagents Cloning primers This study Table EV3 Reagent/resource Reference or source Identifier or Catalogue Number Genotyping PCR primer (SilvaPinheiro et al, 2021) Table EV3 Long-range mtDNA amplification primer (Chi et al, 2012) Table EV3 Primer extension assay primer (Gupta et al, 2014; Hussain et al, 2021) Table EV3 Exonuclease assay primer (Gupta et al, 2014) Table EV3 mtDNA copy number primer N/A Table EV3 Chemicals, enzymes and other reagents IPTG Merck Cat# I6758; CAS Number 367-93-1 Paraformaldehyde Merck Cat# 158127; CAS Number 30525-89-4 PMSF Merck Cat# P7626; CAS Number 329-98-6 Acrylamide Merck Cat# 110784; CAS Number 79-06-1 DTT Merck Cat# D0632; CAS Number 3483-12-3 Sodium deoxycholate Merck Cat# D6750; CAS Number 302-95-4 Tween 20 Sigma-Aldrich Cat# P7949; CAS Number 9005-64-5 Triton X-100 Merck Cat# T9284; CAS Number 9002-93-1 ATP Merck Cat# A9187; CAS Number 74804-12-9 DMSO Merck Cat# D2650; CAS Number 67-68-5 PMSF Sigma-Aldrich Cat# P7626; CAS Number 329-98-6 Imidazole Bio Basic Cat# IB0277; CAS Number 288-32-4 Lysozyme Sigma-Aldrich Cat# L4919; CAS Number 12650-88-3 Proteinase K Bio Basic Cat# PB0451; CAS Number 39450-01-6 L-Glutathione reduced Merck Cat# G6529; CAS Number 70-18-8 N, N′Methylenebisacrylamide Sigma-Aldrich Cat# M7279; CAS Number 110-26-9 Puromycin Sigma-Aldrich Cat# P8833; CAS Number 58-58-2 HEPES Sigma-Aldrich Cat# H3375; CAS Number 7365-45-9 EDTA Merck Cat# 324503; CAS Number 6381-92-6 EGTA Merck Cat# 324626; CAS Number 67-42-5 Na2HPO4 Merck Cat# 106559; CAS Number 7558-79-4 KCl Merck Cat# 104936; CAS Number 7447-40-7 14 EMBO Molecular Medicine © The Author(s) Reagent/resource Reference or source Identifier or Catalogue Number NaCl Merck Cat# 106406; CAS Number 7647-14-5 MgCl2 Sigma-Aldrich Cat# 814733; CAS Number: 7786-30-3 BSA Sigma-Aldrich Cat# SRE0098; CAS Number: 9048-46-8 2-Mercaptoethanol Sigma-Aldrich Cat# 63689; CAS Number: 60-24-2 SDS Sigma-Aldrich Cat# L3771; CAS Number: 151-21-3 Tris(hydroxymethyl) aminomethane Millipore Cat# 108382; CAS Number: 77-86-1 Glycine Millipore Cat# 3570; CAS Number: 56-40-6 Sucrose Sigma-Aldrich Cat# S9378; CAS Number: 57-50-1 Methanol Merck CAS Number: 67-56-1 CCCP Sigma-Aldrich Cat# C2759; CAS Number 555-60-2 BrdU Sigma-Aldrich Cat# B5002; CAS Number 59-14-3 IPTG Sigma-Aldrich Cat# I6758; CAS Number 367-93-1 MG132 Merck Cat# 474787; CAS Number 133407-82-6 Uridine Sigma-Aldrich Cat# U3003; CAS Number 58-96-8 Verapamil hydrochloride Merck CAS Number 152-11-4 Tazobactam Merck CAS Number 89786- 04-9 Clavulanic acid Merck CAS Number 58001- 44-8 D (-)-Mannitol Pure Merck Cat# M9647; CAS Number 69-65-8 Ampicillin Bio Basic Cat# AB0028 Kanamycin Bio Basic Cat# KB0286 Hygromycin B Sigma-Aldrich Cat# H7772 Penicillin-StreptomycinAmphotericin B Solution Biological industries Cat# 03-033-1B KH2PO4 Merck Cat# 104873; CAS Number 7778-77-0 Ethanol Merck Cat# 100983; CAS Number 64-17-5 Glycerol Merck Cat# 107051; CAS Number 56-81-5 TEMED Bio-Rad Cat# 1610801 Ammonium Persulfate Sigma-Aldrich Cat# A3678; CAS Number 7727-54-0 Coomassie brilliant blue-R-250 Bio-Rad Cat# 1610400 Ethidium bromide (EtBr) Sigma-Aldrich Cat# E7637 Luria Bertani Broth, Miller Himedia Cat# M1245 Reagent/resource Reference or source Identifier or Catalogue Number Luria Bertani Agar, Miller Himedia Cat# M1151 Skim milk powder Himedia Cat# GRM1254 Agarose Sigma-Aldrich Cat# A9539 Foetal bovine Serum Thermo Fisher Scientific Cat# 10082147 Advanced DMEM Thermo Fisher Scientific Cat# 12491-023 DMEM/F-12 medium Thermo Fisher Scientific Cat# 11320033 Horse serum Thermo Fisher Scientific Cat# 16050130 OptiMem reduced serum medium Thermo Fisher Scientific Cat# 31985-088 Complete Protease Cocktail inhibitor Roche Cat# 116974980 BL21 (DE3) competent cells Thermo Fisher Scientific Cat# EC0114 Poly-Prep Chromatography column Bio-Rad Cat# 73101550 Mounting medium with DAPI Vector Laboratories Cat# H-1200 Nhe1-HF New England BioLabs Cat# R3131 Not1-HF New England BioLabs Cat# R3189 Spe1-HF New England BioLabs Cat# R3133 XhoI New England BioLabs Cat# R0146 BamHI-HF New England BioLabs Cat# R3136 HindIII-HF New England BioLabs Cat# R3104 Glutathione SSepharose beads Cytiva Cat# 17-5279-02 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 Sepharose CL-6B Sigma-Aldrich Cat# CL6B200 3X Flag peptide Sigma-Aldrich Cat# F4799 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 LongAmp Taq DNA polymerase New England Biolabs Cat# M0323L Immobilon Western Chemiluminescent HRP substrate Merck Cat# WBKLS0500 10Χ PBS Sartorius Cat# 02-023-5 A Trypsin (2.5%) Thermo Fisher Scientific Cat# 15090046 L-Glutamine solution Biological industries Cat# 03-020-1B Prestained Low Molecular weight markers Bio-Rad Cat# 1610374 Wide range DNA marker Sigma-Aldrich Cat# D7058 Nitrocellulose membrane Bio-Rad Cat# 1620115 Streptavidin Agarose Millipore Cat# 69203 T7 Quick-coupled Transcription/ Translation system Promega Cat# L2080 © The Author(s) EMBO Molecular Medicine 15 Reagent/resource Reference or source Identifier or Catalogue Number Software GraphPad Prism 9 https://www.graphpad. .. com/ Other HOOK-Biotin-PEG2Amine Labelling Kit G-Biosciences Cat# BS-16 Mitochondrial DNA isolation kit BioVision Inc. Cat# K280-50 QuikChange II XL SiteDirected Mutagenesis Kit Agilent Cat# 200522 QIAamp DNA Mini Kit Qiagen Cat# 51304 QIAamp DNA Midi Kit Qiagen Cat# 12143 Reagents, antibodies, primers and recombinants All antibodies, reagents (including chemicals, recombinant proteins, cell lines, peptides and oligonucleotides), animals, deposited data and other materials used are described in structured methods. .. com/en/product/cellanalysis/real-time-cellmetabolic-analysis/xfsoftware/seahorsewave-desktop-software740897 FlowJo v10 https://www.flowjo.

    Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study
    Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using QIAamp DNA Mini Kit (QIAGEN, Germany), both according to the manufacturers’ instructions. ..

    Mutagenesis:

    Article Title: Small molecules rescue pathogenic POLGA aggregation and restore its functions in a mouse model of PEO.
    Article Snippet: Polyclonal Antibody, Rabbit polyclonal, living colours antibody (used Takara Cat# 632460 © The Author(s) EMBO Molecular Medicine 13 Reagent/resource Reference or source Identifier or Catalogue Number for YFP) (used for WB, IP) Tom20 (D8T4N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 42406 OPA1 (D6U6N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 80471 DRP1 (D6C7), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 8570 Phospho-DRP1 (Ser616) (D9A1), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 4494 Phospho-DRP1 (Ser637) (D3A4), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 6319 MFF (E5W4M), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 84580 MFN-1 (D6E2S), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 14739 MFN2 (D1E9), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 11925 Anti-Laminin, Rabbit antibody (used for IF) Sigma-Aldrich Cat# L9393 Anti-ATG12 antibody, Rabbit Polyclonal (used for WB) Abcam Cat# ab155589 Anti-Cytochrome C antibody, Mouse monoclonal (used for IF) Novus Biologicals Cat# NB100-56503 COX IV antibody, Rabbit antibody (used for IF) Cell Signalling Technology Cat# 4844 Myc-Tag (71D10), Rabbit monoclonal antibody (used for WB) Cell Signalling Technology Cat# 2278 Anti-His, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 8036 Anti- β actin, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 47778 Anti-GST, Rabbit antibody (used for WB) Thermo Fisher Scientific Cat# 71-7500 DNA Polymerase gamma Antibody; Rabbit Polyclonal (used for IF) Thermo Fisher Scientific Cat# BS-13017R Anti-RECQL4 (used for WB, IP) Santa Cruz Biotechnology Cat# sc-366840 Oligonucleotides and other sequence-based reagents Cloning primers This study Table EV3 Reagent/resource Reference or source Identifier or Catalogue Number Genotyping PCR primer (SilvaPinheiro et al, 2021) Table EV3 Long-range mtDNA amplification primer (Chi et al, 2012) Table EV3 Primer extension assay primer (Gupta et al, 2014; Hussain et al, 2021) Table EV3 Exonuclease assay primer (Gupta et al, 2014) Table EV3 mtDNA copy number primer N/A Table EV3 Chemicals, enzymes and other reagents IPTG Merck Cat# I6758; CAS Number 367-93-1 Paraformaldehyde Merck Cat# 158127; CAS Number 30525-89-4 PMSF Merck Cat# P7626; CAS Number 329-98-6 Acrylamide Merck Cat# 110784; CAS Number 79-06-1 DTT Merck Cat# D0632; CAS Number 3483-12-3 Sodium deoxycholate Merck Cat# D6750; CAS Number 302-95-4 Tween 20 Sigma-Aldrich Cat# P7949; CAS Number 9005-64-5 Triton X-100 Merck Cat# T9284; CAS Number 9002-93-1 ATP Merck Cat# A9187; CAS Number 74804-12-9 DMSO Merck Cat# D2650; CAS Number 67-68-5 PMSF Sigma-Aldrich Cat# P7626; CAS Number 329-98-6 Imidazole Bio Basic Cat# IB0277; CAS Number 288-32-4 Lysozyme Sigma-Aldrich Cat# L4919; CAS Number 12650-88-3 Proteinase K Bio Basic Cat# PB0451; CAS Number 39450-01-6 L-Glutathione reduced Merck Cat# G6529; CAS Number 70-18-8 N, N′Methylenebisacrylamide Sigma-Aldrich Cat# M7279; CAS Number 110-26-9 Puromycin Sigma-Aldrich Cat# P8833; CAS Number 58-58-2 HEPES Sigma-Aldrich Cat# H3375; CAS Number 7365-45-9 EDTA Merck Cat# 324503; CAS Number 6381-92-6 EGTA Merck Cat# 324626; CAS Number 67-42-5 Na2HPO4 Merck Cat# 106559; CAS Number 7558-79-4 KCl Merck Cat# 104936; CAS Number 7447-40-7 14 EMBO Molecular Medicine © The Author(s) Reagent/resource Reference or source Identifier or Catalogue Number NaCl Merck Cat# 106406; CAS Number 7647-14-5 MgCl2 Sigma-Aldrich Cat# 814733; CAS Number: 7786-30-3 BSA Sigma-Aldrich Cat# SRE0098; CAS Number: 9048-46-8 2-Mercaptoethanol Sigma-Aldrich Cat# 63689; CAS Number: 60-24-2 SDS Sigma-Aldrich Cat# L3771; CAS Number: 151-21-3 Tris(hydroxymethyl) aminomethane Millipore Cat# 108382; CAS Number: 77-86-1 Glycine Millipore Cat# 3570; CAS Number: 56-40-6 Sucrose Sigma-Aldrich Cat# S9378; CAS Number: 57-50-1 Methanol Merck CAS Number: 67-56-1 CCCP Sigma-Aldrich Cat# C2759; CAS Number 555-60-2 BrdU Sigma-Aldrich Cat# B5002; CAS Number 59-14-3 IPTG Sigma-Aldrich Cat# I6758; CAS Number 367-93-1 MG132 Merck Cat# 474787; CAS Number 133407-82-6 Uridine Sigma-Aldrich Cat# U3003; CAS Number 58-96-8 Verapamil hydrochloride Merck CAS Number 152-11-4 Tazobactam Merck CAS Number 89786- 04-9 Clavulanic acid Merck CAS Number 58001- 44-8 D (-)-Mannitol Pure Merck Cat# M9647; CAS Number 69-65-8 Ampicillin Bio Basic Cat# AB0028 Kanamycin Bio Basic Cat# KB0286 Hygromycin B Sigma-Aldrich Cat# H7772 Penicillin-StreptomycinAmphotericin B Solution Biological industries Cat# 03-033-1B KH2PO4 Merck Cat# 104873; CAS Number 7778-77-0 Ethanol Merck Cat# 100983; CAS Number 64-17-5 Glycerol Merck Cat# 107051; CAS Number 56-81-5 TEMED Bio-Rad Cat# 1610801 Ammonium Persulfate Sigma-Aldrich Cat# A3678; CAS Number 7727-54-0 Coomassie brilliant blue-R-250 Bio-Rad Cat# 1610400 Ethidium bromide (EtBr) Sigma-Aldrich Cat# E7637 Luria Bertani Broth, Miller Himedia Cat# M1245 Reagent/resource Reference or source Identifier or Catalogue Number Luria Bertani Agar, Miller Himedia Cat# M1151 Skim milk powder Himedia Cat# GRM1254 Agarose Sigma-Aldrich Cat# A9539 Foetal bovine Serum Thermo Fisher Scientific Cat# 10082147 Advanced DMEM Thermo Fisher Scientific Cat# 12491-023 DMEM/F-12 medium Thermo Fisher Scientific Cat# 11320033 Horse serum Thermo Fisher Scientific Cat# 16050130 OptiMem reduced serum medium Thermo Fisher Scientific Cat# 31985-088 Complete Protease Cocktail inhibitor Roche Cat# 116974980 BL21 (DE3) competent cells Thermo Fisher Scientific Cat# EC0114 Poly-Prep Chromatography column Bio-Rad Cat# 73101550 Mounting medium with DAPI Vector Laboratories Cat# H-1200 Nhe1-HF New England BioLabs Cat# R3131 Not1-HF New England BioLabs Cat# R3189 Spe1-HF New England BioLabs Cat# R3133 XhoI New England BioLabs Cat# R0146 BamHI-HF New England BioLabs Cat# R3136 HindIII-HF New England BioLabs Cat# R3104 Glutathione SSepharose beads Cytiva Cat# 17-5279-02 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 Sepharose CL-6B Sigma-Aldrich Cat# CL6B200 3X Flag peptide Sigma-Aldrich Cat# F4799 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 LongAmp Taq DNA polymerase New England Biolabs Cat# M0323L Immobilon Western Chemiluminescent HRP substrate Merck Cat# WBKLS0500 10Χ PBS Sartorius Cat# 02-023-5 A Trypsin (2.5%) Thermo Fisher Scientific Cat# 15090046 L-Glutamine solution Biological industries Cat# 03-020-1B Prestained Low Molecular weight markers Bio-Rad Cat# 1610374 Wide range DNA marker Sigma-Aldrich Cat# D7058 Nitrocellulose membrane Bio-Rad Cat# 1620115 Streptavidin Agarose Millipore Cat# 69203 T7 Quick-coupled Transcription/ Translation system Promega Cat# L2080 © The Author(s) EMBO Molecular Medicine 15 Reagent/resource Reference or source Identifier or Catalogue Number Software GraphPad Prism 9 https://www.graphpad. .. com/ Other HOOK-Biotin-PEG2Amine Labelling Kit G-Biosciences Cat# BS-16 Mitochondrial DNA isolation kit BioVision Inc. Cat# K280-50 QuikChange II XL SiteDirected Mutagenesis Kit Agilent Cat# 200522 QIAamp DNA Mini Kit Qiagen Cat# 51304 QIAamp DNA Midi Kit Qiagen Cat# 12143 Reagents, antibodies, primers and recombinants All antibodies, reagents (including chemicals, recombinant proteins, cell lines, peptides and oligonucleotides), animals, deposited data and other materials used are described in structured methods. .. com/en/product/cellanalysis/real-time-cellmetabolic-analysis/xfsoftware/seahorsewave-desktop-software740897 FlowJo v10 https://www.flowjo.

    Recombinant:

    Article Title: Small molecules rescue pathogenic POLGA aggregation and restore its functions in a mouse model of PEO.
    Article Snippet: Polyclonal Antibody, Rabbit polyclonal, living colours antibody (used Takara Cat# 632460 © The Author(s) EMBO Molecular Medicine 13 Reagent/resource Reference or source Identifier or Catalogue Number for YFP) (used for WB, IP) Tom20 (D8T4N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 42406 OPA1 (D6U6N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 80471 DRP1 (D6C7), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 8570 Phospho-DRP1 (Ser616) (D9A1), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 4494 Phospho-DRP1 (Ser637) (D3A4), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 6319 MFF (E5W4M), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 84580 MFN-1 (D6E2S), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 14739 MFN2 (D1E9), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 11925 Anti-Laminin, Rabbit antibody (used for IF) Sigma-Aldrich Cat# L9393 Anti-ATG12 antibody, Rabbit Polyclonal (used for WB) Abcam Cat# ab155589 Anti-Cytochrome C antibody, Mouse monoclonal (used for IF) Novus Biologicals Cat# NB100-56503 COX IV antibody, Rabbit antibody (used for IF) Cell Signalling Technology Cat# 4844 Myc-Tag (71D10), Rabbit monoclonal antibody (used for WB) Cell Signalling Technology Cat# 2278 Anti-His, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 8036 Anti- β actin, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 47778 Anti-GST, Rabbit antibody (used for WB) Thermo Fisher Scientific Cat# 71-7500 DNA Polymerase gamma Antibody; Rabbit Polyclonal (used for IF) Thermo Fisher Scientific Cat# BS-13017R Anti-RECQL4 (used for WB, IP) Santa Cruz Biotechnology Cat# sc-366840 Oligonucleotides and other sequence-based reagents Cloning primers This study Table EV3 Reagent/resource Reference or source Identifier or Catalogue Number Genotyping PCR primer (SilvaPinheiro et al, 2021) Table EV3 Long-range mtDNA amplification primer (Chi et al, 2012) Table EV3 Primer extension assay primer (Gupta et al, 2014; Hussain et al, 2021) Table EV3 Exonuclease assay primer (Gupta et al, 2014) Table EV3 mtDNA copy number primer N/A Table EV3 Chemicals, enzymes and other reagents IPTG Merck Cat# I6758; CAS Number 367-93-1 Paraformaldehyde Merck Cat# 158127; CAS Number 30525-89-4 PMSF Merck Cat# P7626; CAS Number 329-98-6 Acrylamide Merck Cat# 110784; CAS Number 79-06-1 DTT Merck Cat# D0632; CAS Number 3483-12-3 Sodium deoxycholate Merck Cat# D6750; CAS Number 302-95-4 Tween 20 Sigma-Aldrich Cat# P7949; CAS Number 9005-64-5 Triton X-100 Merck Cat# T9284; CAS Number 9002-93-1 ATP Merck Cat# A9187; CAS Number 74804-12-9 DMSO Merck Cat# D2650; CAS Number 67-68-5 PMSF Sigma-Aldrich Cat# P7626; CAS Number 329-98-6 Imidazole Bio Basic Cat# IB0277; CAS Number 288-32-4 Lysozyme Sigma-Aldrich Cat# L4919; CAS Number 12650-88-3 Proteinase K Bio Basic Cat# PB0451; CAS Number 39450-01-6 L-Glutathione reduced Merck Cat# G6529; CAS Number 70-18-8 N, N′Methylenebisacrylamide Sigma-Aldrich Cat# M7279; CAS Number 110-26-9 Puromycin Sigma-Aldrich Cat# P8833; CAS Number 58-58-2 HEPES Sigma-Aldrich Cat# H3375; CAS Number 7365-45-9 EDTA Merck Cat# 324503; CAS Number 6381-92-6 EGTA Merck Cat# 324626; CAS Number 67-42-5 Na2HPO4 Merck Cat# 106559; CAS Number 7558-79-4 KCl Merck Cat# 104936; CAS Number 7447-40-7 14 EMBO Molecular Medicine © The Author(s) Reagent/resource Reference or source Identifier or Catalogue Number NaCl Merck Cat# 106406; CAS Number 7647-14-5 MgCl2 Sigma-Aldrich Cat# 814733; CAS Number: 7786-30-3 BSA Sigma-Aldrich Cat# SRE0098; CAS Number: 9048-46-8 2-Mercaptoethanol Sigma-Aldrich Cat# 63689; CAS Number: 60-24-2 SDS Sigma-Aldrich Cat# L3771; CAS Number: 151-21-3 Tris(hydroxymethyl) aminomethane Millipore Cat# 108382; CAS Number: 77-86-1 Glycine Millipore Cat# 3570; CAS Number: 56-40-6 Sucrose Sigma-Aldrich Cat# S9378; CAS Number: 57-50-1 Methanol Merck CAS Number: 67-56-1 CCCP Sigma-Aldrich Cat# C2759; CAS Number 555-60-2 BrdU Sigma-Aldrich Cat# B5002; CAS Number 59-14-3 IPTG Sigma-Aldrich Cat# I6758; CAS Number 367-93-1 MG132 Merck Cat# 474787; CAS Number 133407-82-6 Uridine Sigma-Aldrich Cat# U3003; CAS Number 58-96-8 Verapamil hydrochloride Merck CAS Number 152-11-4 Tazobactam Merck CAS Number 89786- 04-9 Clavulanic acid Merck CAS Number 58001- 44-8 D (-)-Mannitol Pure Merck Cat# M9647; CAS Number 69-65-8 Ampicillin Bio Basic Cat# AB0028 Kanamycin Bio Basic Cat# KB0286 Hygromycin B Sigma-Aldrich Cat# H7772 Penicillin-StreptomycinAmphotericin B Solution Biological industries Cat# 03-033-1B KH2PO4 Merck Cat# 104873; CAS Number 7778-77-0 Ethanol Merck Cat# 100983; CAS Number 64-17-5 Glycerol Merck Cat# 107051; CAS Number 56-81-5 TEMED Bio-Rad Cat# 1610801 Ammonium Persulfate Sigma-Aldrich Cat# A3678; CAS Number 7727-54-0 Coomassie brilliant blue-R-250 Bio-Rad Cat# 1610400 Ethidium bromide (EtBr) Sigma-Aldrich Cat# E7637 Luria Bertani Broth, Miller Himedia Cat# M1245 Reagent/resource Reference or source Identifier or Catalogue Number Luria Bertani Agar, Miller Himedia Cat# M1151 Skim milk powder Himedia Cat# GRM1254 Agarose Sigma-Aldrich Cat# A9539 Foetal bovine Serum Thermo Fisher Scientific Cat# 10082147 Advanced DMEM Thermo Fisher Scientific Cat# 12491-023 DMEM/F-12 medium Thermo Fisher Scientific Cat# 11320033 Horse serum Thermo Fisher Scientific Cat# 16050130 OptiMem reduced serum medium Thermo Fisher Scientific Cat# 31985-088 Complete Protease Cocktail inhibitor Roche Cat# 116974980 BL21 (DE3) competent cells Thermo Fisher Scientific Cat# EC0114 Poly-Prep Chromatography column Bio-Rad Cat# 73101550 Mounting medium with DAPI Vector Laboratories Cat# H-1200 Nhe1-HF New England BioLabs Cat# R3131 Not1-HF New England BioLabs Cat# R3189 Spe1-HF New England BioLabs Cat# R3133 XhoI New England BioLabs Cat# R0146 BamHI-HF New England BioLabs Cat# R3136 HindIII-HF New England BioLabs Cat# R3104 Glutathione SSepharose beads Cytiva Cat# 17-5279-02 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 Sepharose CL-6B Sigma-Aldrich Cat# CL6B200 3X Flag peptide Sigma-Aldrich Cat# F4799 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 LongAmp Taq DNA polymerase New England Biolabs Cat# M0323L Immobilon Western Chemiluminescent HRP substrate Merck Cat# WBKLS0500 10Χ PBS Sartorius Cat# 02-023-5 A Trypsin (2.5%) Thermo Fisher Scientific Cat# 15090046 L-Glutamine solution Biological industries Cat# 03-020-1B Prestained Low Molecular weight markers Bio-Rad Cat# 1610374 Wide range DNA marker Sigma-Aldrich Cat# D7058 Nitrocellulose membrane Bio-Rad Cat# 1620115 Streptavidin Agarose Millipore Cat# 69203 T7 Quick-coupled Transcription/ Translation system Promega Cat# L2080 © The Author(s) EMBO Molecular Medicine 15 Reagent/resource Reference or source Identifier or Catalogue Number Software GraphPad Prism 9 https://www.graphpad. .. com/ Other HOOK-Biotin-PEG2Amine Labelling Kit G-Biosciences Cat# BS-16 Mitochondrial DNA isolation kit BioVision Inc. Cat# K280-50 QuikChange II XL SiteDirected Mutagenesis Kit Agilent Cat# 200522 QIAamp DNA Mini Kit Qiagen Cat# 51304 QIAamp DNA Midi Kit Qiagen Cat# 12143 Reagents, antibodies, primers and recombinants All antibodies, reagents (including chemicals, recombinant proteins, cell lines, peptides and oligonucleotides), animals, deposited data and other materials used are described in structured methods. .. com/en/product/cellanalysis/real-time-cellmetabolic-analysis/xfsoftware/seahorsewave-desktop-software740897 FlowJo v10 https://www.flowjo.

    Qubit:

    Article Title: Consistent temperature-dependent effects on multiple displacement amplification.
    Article Snippet: Multiple displacement amplification (MDA) is widely used in the sample preparation steps of sequencing, but the amplification bias remains a major obstacle to its application.. While elevating reaction temperature has been reported to improve MDA performance, the strand thermodynamics across various temperatures remain poorly understood.. Through a comparative analysis of the sequencing data generated from MDA reactions performed at different temperatures, we characterized the temperature-dependent patterns of genomic bias and found that performing MDA at 38C optimizes coverage uniformity for complex human genomic samples.

    Sensitivity:

    Article Title: Consistent temperature-dependent effects on multiple displacement amplification.
    Article Snippet: Multiple displacement amplification (MDA) is widely used in the sample preparation steps of sequencing, but the amplification bias remains a major obstacle to its application.. While elevating reaction temperature has been reported to improve MDA performance, the strand thermodynamics across various temperatures remain poorly understood.. Through a comparative analysis of the sequencing data generated from MDA reactions performed at different temperatures, we characterized the temperature-dependent patterns of genomic bias and found that performing MDA at 38C optimizes coverage uniformity for complex human genomic samples.

    Isolation:

    Article Title: Highly multiplexed mammalian metabolic engineering with a shotgun approach.
    Article Snippet: .. gDNA was isolated from 3–6 million cells using either the QIAamp DNA mini kit (Qiagen) or the Monarch genomic purification kit (New England Biolabs), incorporating an RNase treatment phase in all instances. .. To achieve target fragment lengths, 2 μg of gDNA was diluted in 150 μl of nuclease-free water and sheared using g-TUBE (Covaris) at 3,000g.

    Purification:

    Article Title: Highly multiplexed mammalian metabolic engineering with a shotgun approach.
    Article Snippet: .. gDNA was isolated from 3–6 million cells using either the QIAamp DNA mini kit (Qiagen) or the Monarch genomic purification kit (New England Biolabs), incorporating an RNase treatment phase in all instances. .. To achieve target fragment lengths, 2 μg of gDNA was diluted in 150 μl of nuclease-free water and sheared using g-TUBE (Covaris) at 3,000g.

    Article Title: Clinical Evaluation of a Stool‐Based Molecular Point‐of‐Care Test Kit for Noninvasive Detection of Helicobacter pylori Infection and Clarithromycin Resistance
    Article Snippet: .. For stool, DNA was extracted from 100 μL of stool collection liquid with the QIAamp DNA Mini Kit (QIAGEN GmbH, Hilden, Germany) and concentrated with the NucleoSpin Gel and PCR Clean‐up (Takara Bio, Kusatsu, Japan) to obtain 20 μL of purified DNA. ..

    Whole-Genome Sequencing:

    Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study
    Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using QIAamp DNA Mini Kit (QIAGEN, Germany), both according to the manufacturers’ instructions. ..

    Whole Complete genome sequencing:

    Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study
    Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using QIAamp DNA Mini Kit (QIAGEN, Germany), both according to the manufacturers’ instructions. ..

    Analysis:

    Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study
    Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using QIAamp DNA Mini Kit (QIAGEN, Germany), both according to the manufacturers’ instructions. ..

    Cell Culture:

    Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study
    Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using QIAamp DNA Mini Kit (QIAGEN, Germany), both according to the manufacturers’ instructions. ..

    Polymerase Chain Reaction:

    Article Title: Clinical Evaluation of a Stool‐Based Molecular Point‐of‐Care Test Kit for Noninvasive Detection of Helicobacter pylori Infection and Clarithromycin Resistance
    Article Snippet: .. For stool, DNA was extracted from 100 μL of stool collection liquid with the QIAamp DNA Mini Kit (QIAGEN GmbH, Hilden, Germany) and concentrated with the NucleoSpin Gel and PCR Clean‐up (Takara Bio, Kusatsu, Japan) to obtain 20 μL of purified DNA. ..



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    TABLE 5

    Journal: Biology of Reproduction

    Article Title: Novel Targets for the Transcription Factors MEF2 in MA-10 Leydig Cells 1

    doi: 10.1095/biolreprod.114.127761

    Figure Lengend Snippet: TABLE 5

    Article Snippet: Sample Labeling and Microarray Hybridization DNA microarray analyses were performed using Affymetrix Mouse Gene 1.0 ST arrays.

    Techniques: Comparison, Microarray