qiaamp dna mini stool kit (Qiagen)
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Qiagen
qiaamp dna mini stool kit
Qiaamp Dna Mini Stool Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 56073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridization+dna+microarray+analyses/QIAamp+DNA+Mini+Kit/pm23319531-57-20-25
Average 99 stars, based on 56073 article reviews
Qiaamp Dna Mini Stool Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 56073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridization+dna+microarray+analyses/QIAamp+DNA+Mini+Kit/pm23319531-57-20-25
Average 99 stars, based on 56073 article reviews
qiaamp dna mini stool kit - by Bioz Stars,
2026-10
99/100 stars
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DNA Extraction:Article Title: Small molecules rescue pathogenic POLGA aggregation and restore its functions in a mouse model of PEO. Article Snippet: Polyclonal Antibody, Rabbit polyclonal, living colours antibody (used Takara Cat# 632460 © The Author(s) EMBO Molecular Medicine 13 Reagent/resource Reference or source Identifier or Catalogue Number for YFP) (used for WB, IP) Tom20 (D8T4N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 42406 OPA1 (D6U6N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 80471 DRP1 (D6C7), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 8570 Phospho-DRP1 (Ser616) (D9A1), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 4494 Phospho-DRP1 (Ser637) (D3A4), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 6319 MFF (E5W4M), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 84580 MFN-1 (D6E2S), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 14739 MFN2 (D1E9), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 11925 Anti-Laminin, Rabbit antibody (used for IF) Sigma-Aldrich Cat# L9393 Anti-ATG12 antibody, Rabbit Polyclonal (used for WB) Abcam Cat# ab155589 Anti-Cytochrome C antibody, Mouse monoclonal (used for IF) Novus Biologicals Cat# NB100-56503 COX IV antibody, Rabbit antibody (used for IF) Cell Signalling Technology Cat# 4844 Myc-Tag (71D10), Rabbit monoclonal antibody (used for WB) Cell Signalling Technology Cat# 2278 Anti-His, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 8036 Anti- β actin, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 47778 Anti-GST, Rabbit antibody (used for WB) Thermo Fisher Scientific Cat# 71-7500 DNA Polymerase gamma Antibody; Rabbit Polyclonal (used for IF) Thermo Fisher Scientific Cat# BS-13017R Anti-RECQL4 (used for WB, IP) Santa Cruz Biotechnology Cat# sc-366840 Oligonucleotides and other sequence-based reagents Cloning primers This study Table EV3 Reagent/resource Reference or source Identifier or Catalogue Number Genotyping PCR primer (SilvaPinheiro et al, 2021) Table EV3 Long-range mtDNA amplification primer (Chi et al, 2012) Table EV3 Primer extension assay primer (Gupta et al, 2014; Hussain et al, 2021) Table EV3 Exonuclease assay primer (Gupta et al, 2014) Table EV3 mtDNA copy number primer N/A Table EV3 Chemicals, enzymes and other reagents IPTG Merck Cat# I6758; CAS Number 367-93-1 Paraformaldehyde Merck Cat# 158127; CAS Number 30525-89-4 PMSF Merck Cat# P7626; CAS Number 329-98-6 Acrylamide Merck Cat# 110784; CAS Number 79-06-1 DTT Merck Cat# D0632; CAS Number 3483-12-3 Sodium deoxycholate Merck Cat# D6750; CAS Number 302-95-4 Tween 20 Sigma-Aldrich Cat# P7949; CAS Number 9005-64-5 Triton X-100 Merck Cat# T9284; CAS Number 9002-93-1 ATP Merck Cat# A9187; CAS Number 74804-12-9 DMSO Merck Cat# D2650; CAS Number 67-68-5 PMSF Sigma-Aldrich Cat# P7626; CAS Number 329-98-6 Imidazole Bio Basic Cat# IB0277; CAS Number 288-32-4 Lysozyme Sigma-Aldrich Cat# L4919; CAS Number 12650-88-3 Proteinase K Bio Basic Cat# PB0451; CAS Number 39450-01-6 L-Glutathione reduced Merck Cat# G6529; CAS Number 70-18-8 N, N′Methylenebisacrylamide Sigma-Aldrich Cat# M7279; CAS Number 110-26-9 Puromycin Sigma-Aldrich Cat# P8833; CAS Number 58-58-2 HEPES Sigma-Aldrich Cat# H3375; CAS Number 7365-45-9 EDTA Merck Cat# 324503; CAS Number 6381-92-6 EGTA Merck Cat# 324626; CAS Number 67-42-5 Na2HPO4 Merck Cat# 106559; CAS Number 7558-79-4 KCl Merck Cat# 104936; CAS Number 7447-40-7 14 EMBO Molecular Medicine © The Author(s) Reagent/resource Reference or source Identifier or Catalogue Number NaCl Merck Cat# 106406; CAS Number 7647-14-5 MgCl2 Sigma-Aldrich Cat# 814733; CAS Number: 7786-30-3 BSA Sigma-Aldrich Cat# SRE0098; CAS Number: 9048-46-8 2-Mercaptoethanol Sigma-Aldrich Cat# 63689; CAS Number: 60-24-2 SDS Sigma-Aldrich Cat# L3771; CAS Number: 151-21-3 Tris(hydroxymethyl) aminomethane Millipore Cat# 108382; CAS Number: 77-86-1 Glycine Millipore Cat# 3570; CAS Number: 56-40-6 Sucrose Sigma-Aldrich Cat# S9378; CAS Number: 57-50-1 Methanol Merck CAS Number: 67-56-1 CCCP Sigma-Aldrich Cat# C2759; CAS Number 555-60-2 BrdU Sigma-Aldrich Cat# B5002; CAS Number 59-14-3 IPTG Sigma-Aldrich Cat# I6758; CAS Number 367-93-1 MG132 Merck Cat# 474787; CAS Number 133407-82-6 Uridine Sigma-Aldrich Cat# U3003; CAS Number 58-96-8 Verapamil hydrochloride Merck CAS Number 152-11-4 Tazobactam Merck CAS Number 89786- 04-9 Clavulanic acid Merck CAS Number 58001- 44-8 D (-)-Mannitol Pure Merck Cat# M9647; CAS Number 69-65-8 Ampicillin Bio Basic Cat# AB0028 Kanamycin Bio Basic Cat# KB0286 Hygromycin B Sigma-Aldrich Cat# H7772 Penicillin-StreptomycinAmphotericin B Solution Biological industries Cat# 03-033-1B KH2PO4 Merck Cat# 104873; CAS Number 7778-77-0 Ethanol Merck Cat# 100983; CAS Number 64-17-5 Glycerol Merck Cat# 107051; CAS Number 56-81-5 TEMED Bio-Rad Cat# 1610801 Ammonium Persulfate Sigma-Aldrich Cat# A3678; CAS Number 7727-54-0 Coomassie brilliant blue-R-250 Bio-Rad Cat# 1610400 Ethidium bromide (EtBr) Sigma-Aldrich Cat# E7637 Luria Bertani Broth, Miller Himedia Cat# M1245 Reagent/resource Reference or source Identifier or Catalogue Number Luria Bertani Agar, Miller Himedia Cat# M1151 Skim milk powder Himedia Cat# GRM1254 Agarose Sigma-Aldrich Cat# A9539 Foetal bovine Serum Thermo Fisher Scientific Cat# 10082147 Advanced DMEM Thermo Fisher Scientific Cat# 12491-023 DMEM/F-12 medium Thermo Fisher Scientific Cat# 11320033 Horse serum Thermo Fisher Scientific Cat# 16050130 OptiMem reduced serum medium Thermo Fisher Scientific Cat# 31985-088 Complete Protease Cocktail inhibitor Roche Cat# 116974980 BL21 (DE3) competent cells Thermo Fisher Scientific Cat# EC0114 Poly-Prep Chromatography column Bio-Rad Cat# 73101550 Mounting medium with DAPI Vector Laboratories Cat# H-1200 Nhe1-HF New England BioLabs Cat# R3131 Not1-HF New England BioLabs Cat# R3189 Spe1-HF New England BioLabs Cat# R3133 XhoI New England BioLabs Cat# R0146 BamHI-HF New England BioLabs Cat# R3136 HindIII-HF New England BioLabs Cat# R3104 Glutathione SSepharose beads Cytiva Cat# 17-5279-02 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 Sepharose CL-6B Sigma-Aldrich Cat# CL6B200 3X Flag peptide Sigma-Aldrich Cat# F4799 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 LongAmp Taq DNA polymerase New England Biolabs Cat# M0323L Immobilon Western Chemiluminescent HRP substrate Merck Cat# WBKLS0500 10Χ PBS Sartorius Cat# 02-023-5 A Trypsin (2.5%) Thermo Fisher Scientific Cat# 15090046 L-Glutamine solution Biological industries Cat# 03-020-1B Prestained Low Molecular weight markers Bio-Rad Cat# 1610374 Wide range DNA marker Sigma-Aldrich Cat# D7058 Nitrocellulose membrane Bio-Rad Cat# 1620115 Streptavidin Agarose Millipore Cat# 69203 T7 Quick-coupled Transcription/ Translation system Promega Cat# L2080 © The Author(s) EMBO Molecular Medicine 15 Reagent/resource Reference or source Identifier or Catalogue Number Software GraphPad Prism 9 https://www.graphpad. .. com/ Other HOOK-Biotin-PEG2Amine Labelling Kit G-Biosciences Cat# BS-16 Mitochondrial DNA isolation kit BioVision Inc. Cat# K280-50 QuikChange II XL SiteDirected Mutagenesis Kit Agilent Cat# 200522 Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using Mutagenesis:Article Title: Small molecules rescue pathogenic POLGA aggregation and restore its functions in a mouse model of PEO. Article Snippet: Polyclonal Antibody, Rabbit polyclonal, living colours antibody (used Takara Cat# 632460 © The Author(s) EMBO Molecular Medicine 13 Reagent/resource Reference or source Identifier or Catalogue Number for YFP) (used for WB, IP) Tom20 (D8T4N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 42406 OPA1 (D6U6N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 80471 DRP1 (D6C7), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 8570 Phospho-DRP1 (Ser616) (D9A1), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 4494 Phospho-DRP1 (Ser637) (D3A4), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 6319 MFF (E5W4M), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 84580 MFN-1 (D6E2S), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 14739 MFN2 (D1E9), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 11925 Anti-Laminin, Rabbit antibody (used for IF) Sigma-Aldrich Cat# L9393 Anti-ATG12 antibody, Rabbit Polyclonal (used for WB) Abcam Cat# ab155589 Anti-Cytochrome C antibody, Mouse monoclonal (used for IF) Novus Biologicals Cat# NB100-56503 COX IV antibody, Rabbit antibody (used for IF) Cell Signalling Technology Cat# 4844 Myc-Tag (71D10), Rabbit monoclonal antibody (used for WB) Cell Signalling Technology Cat# 2278 Anti-His, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 8036 Anti- β actin, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 47778 Anti-GST, Rabbit antibody (used for WB) Thermo Fisher Scientific Cat# 71-7500 DNA Polymerase gamma Antibody; Rabbit Polyclonal (used for IF) Thermo Fisher Scientific Cat# BS-13017R Anti-RECQL4 (used for WB, IP) Santa Cruz Biotechnology Cat# sc-366840 Oligonucleotides and other sequence-based reagents Cloning primers This study Table EV3 Reagent/resource Reference or source Identifier or Catalogue Number Genotyping PCR primer (SilvaPinheiro et al, 2021) Table EV3 Long-range mtDNA amplification primer (Chi et al, 2012) Table EV3 Primer extension assay primer (Gupta et al, 2014; Hussain et al, 2021) Table EV3 Exonuclease assay primer (Gupta et al, 2014) Table EV3 mtDNA copy number primer N/A Table EV3 Chemicals, enzymes and other reagents IPTG Merck Cat# I6758; CAS Number 367-93-1 Paraformaldehyde Merck Cat# 158127; CAS Number 30525-89-4 PMSF Merck Cat# P7626; CAS Number 329-98-6 Acrylamide Merck Cat# 110784; CAS Number 79-06-1 DTT Merck Cat# D0632; CAS Number 3483-12-3 Sodium deoxycholate Merck Cat# D6750; CAS Number 302-95-4 Tween 20 Sigma-Aldrich Cat# P7949; CAS Number 9005-64-5 Triton X-100 Merck Cat# T9284; CAS Number 9002-93-1 ATP Merck Cat# A9187; CAS Number 74804-12-9 DMSO Merck Cat# D2650; CAS Number 67-68-5 PMSF Sigma-Aldrich Cat# P7626; CAS Number 329-98-6 Imidazole Bio Basic Cat# IB0277; CAS Number 288-32-4 Lysozyme Sigma-Aldrich Cat# L4919; CAS Number 12650-88-3 Proteinase K Bio Basic Cat# PB0451; CAS Number 39450-01-6 L-Glutathione reduced Merck Cat# G6529; CAS Number 70-18-8 N, N′Methylenebisacrylamide Sigma-Aldrich Cat# M7279; CAS Number 110-26-9 Puromycin Sigma-Aldrich Cat# P8833; CAS Number 58-58-2 HEPES Sigma-Aldrich Cat# H3375; CAS Number 7365-45-9 EDTA Merck Cat# 324503; CAS Number 6381-92-6 EGTA Merck Cat# 324626; CAS Number 67-42-5 Na2HPO4 Merck Cat# 106559; CAS Number 7558-79-4 KCl Merck Cat# 104936; CAS Number 7447-40-7 14 EMBO Molecular Medicine © The Author(s) Reagent/resource Reference or source Identifier or Catalogue Number NaCl Merck Cat# 106406; CAS Number 7647-14-5 MgCl2 Sigma-Aldrich Cat# 814733; CAS Number: 7786-30-3 BSA Sigma-Aldrich Cat# SRE0098; CAS Number: 9048-46-8 2-Mercaptoethanol Sigma-Aldrich Cat# 63689; CAS Number: 60-24-2 SDS Sigma-Aldrich Cat# L3771; CAS Number: 151-21-3 Tris(hydroxymethyl) aminomethane Millipore Cat# 108382; CAS Number: 77-86-1 Glycine Millipore Cat# 3570; CAS Number: 56-40-6 Sucrose Sigma-Aldrich Cat# S9378; CAS Number: 57-50-1 Methanol Merck CAS Number: 67-56-1 CCCP Sigma-Aldrich Cat# C2759; CAS Number 555-60-2 BrdU Sigma-Aldrich Cat# B5002; CAS Number 59-14-3 IPTG Sigma-Aldrich Cat# I6758; CAS Number 367-93-1 MG132 Merck Cat# 474787; CAS Number 133407-82-6 Uridine Sigma-Aldrich Cat# U3003; CAS Number 58-96-8 Verapamil hydrochloride Merck CAS Number 152-11-4 Tazobactam Merck CAS Number 89786- 04-9 Clavulanic acid Merck CAS Number 58001- 44-8 D (-)-Mannitol Pure Merck Cat# M9647; CAS Number 69-65-8 Ampicillin Bio Basic Cat# AB0028 Kanamycin Bio Basic Cat# KB0286 Hygromycin B Sigma-Aldrich Cat# H7772 Penicillin-StreptomycinAmphotericin B Solution Biological industries Cat# 03-033-1B KH2PO4 Merck Cat# 104873; CAS Number 7778-77-0 Ethanol Merck Cat# 100983; CAS Number 64-17-5 Glycerol Merck Cat# 107051; CAS Number 56-81-5 TEMED Bio-Rad Cat# 1610801 Ammonium Persulfate Sigma-Aldrich Cat# A3678; CAS Number 7727-54-0 Coomassie brilliant blue-R-250 Bio-Rad Cat# 1610400 Ethidium bromide (EtBr) Sigma-Aldrich Cat# E7637 Luria Bertani Broth, Miller Himedia Cat# M1245 Reagent/resource Reference or source Identifier or Catalogue Number Luria Bertani Agar, Miller Himedia Cat# M1151 Skim milk powder Himedia Cat# GRM1254 Agarose Sigma-Aldrich Cat# A9539 Foetal bovine Serum Thermo Fisher Scientific Cat# 10082147 Advanced DMEM Thermo Fisher Scientific Cat# 12491-023 DMEM/F-12 medium Thermo Fisher Scientific Cat# 11320033 Horse serum Thermo Fisher Scientific Cat# 16050130 OptiMem reduced serum medium Thermo Fisher Scientific Cat# 31985-088 Complete Protease Cocktail inhibitor Roche Cat# 116974980 BL21 (DE3) competent cells Thermo Fisher Scientific Cat# EC0114 Poly-Prep Chromatography column Bio-Rad Cat# 73101550 Mounting medium with DAPI Vector Laboratories Cat# H-1200 Nhe1-HF New England BioLabs Cat# R3131 Not1-HF New England BioLabs Cat# R3189 Spe1-HF New England BioLabs Cat# R3133 XhoI New England BioLabs Cat# R0146 BamHI-HF New England BioLabs Cat# R3136 HindIII-HF New England BioLabs Cat# R3104 Glutathione SSepharose beads Cytiva Cat# 17-5279-02 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 Sepharose CL-6B Sigma-Aldrich Cat# CL6B200 3X Flag peptide Sigma-Aldrich Cat# F4799 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 LongAmp Taq DNA polymerase New England Biolabs Cat# M0323L Immobilon Western Chemiluminescent HRP substrate Merck Cat# WBKLS0500 10Χ PBS Sartorius Cat# 02-023-5 A Trypsin (2.5%) Thermo Fisher Scientific Cat# 15090046 L-Glutamine solution Biological industries Cat# 03-020-1B Prestained Low Molecular weight markers Bio-Rad Cat# 1610374 Wide range DNA marker Sigma-Aldrich Cat# D7058 Nitrocellulose membrane Bio-Rad Cat# 1620115 Streptavidin Agarose Millipore Cat# 69203 T7 Quick-coupled Transcription/ Translation system Promega Cat# L2080 © The Author(s) EMBO Molecular Medicine 15 Reagent/resource Reference or source Identifier or Catalogue Number Software GraphPad Prism 9 https://www.graphpad. .. com/ Other HOOK-Biotin-PEG2Amine Labelling Kit G-Biosciences Cat# BS-16 Mitochondrial DNA isolation kit BioVision Inc. Cat# K280-50 QuikChange II XL SiteDirected Mutagenesis Kit Agilent Cat# 200522 Recombinant:Article Title: Small molecules rescue pathogenic POLGA aggregation and restore its functions in a mouse model of PEO. Article Snippet: Polyclonal Antibody, Rabbit polyclonal, living colours antibody (used Takara Cat# 632460 © The Author(s) EMBO Molecular Medicine 13 Reagent/resource Reference or source Identifier or Catalogue Number for YFP) (used for WB, IP) Tom20 (D8T4N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 42406 OPA1 (D6U6N), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 80471 DRP1 (D6C7), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 8570 Phospho-DRP1 (Ser616) (D9A1), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 4494 Phospho-DRP1 (Ser637) (D3A4), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 6319 MFF (E5W4M), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 84580 MFN-1 (D6E2S), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 14739 MFN2 (D1E9), Rabbit monoclonal (used for WB) Cell Signalling Technology Cat# 11925 Anti-Laminin, Rabbit antibody (used for IF) Sigma-Aldrich Cat# L9393 Anti-ATG12 antibody, Rabbit Polyclonal (used for WB) Abcam Cat# ab155589 Anti-Cytochrome C antibody, Mouse monoclonal (used for IF) Novus Biologicals Cat# NB100-56503 COX IV antibody, Rabbit antibody (used for IF) Cell Signalling Technology Cat# 4844 Myc-Tag (71D10), Rabbit monoclonal antibody (used for WB) Cell Signalling Technology Cat# 2278 Anti-His, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 8036 Anti- β actin, Mouse monoclonal antibody (used for WB) Santa Cruz Biotechnology Cat# Sc 47778 Anti-GST, Rabbit antibody (used for WB) Thermo Fisher Scientific Cat# 71-7500 DNA Polymerase gamma Antibody; Rabbit Polyclonal (used for IF) Thermo Fisher Scientific Cat# BS-13017R Anti-RECQL4 (used for WB, IP) Santa Cruz Biotechnology Cat# sc-366840 Oligonucleotides and other sequence-based reagents Cloning primers This study Table EV3 Reagent/resource Reference or source Identifier or Catalogue Number Genotyping PCR primer (SilvaPinheiro et al, 2021) Table EV3 Long-range mtDNA amplification primer (Chi et al, 2012) Table EV3 Primer extension assay primer (Gupta et al, 2014; Hussain et al, 2021) Table EV3 Exonuclease assay primer (Gupta et al, 2014) Table EV3 mtDNA copy number primer N/A Table EV3 Chemicals, enzymes and other reagents IPTG Merck Cat# I6758; CAS Number 367-93-1 Paraformaldehyde Merck Cat# 158127; CAS Number 30525-89-4 PMSF Merck Cat# P7626; CAS Number 329-98-6 Acrylamide Merck Cat# 110784; CAS Number 79-06-1 DTT Merck Cat# D0632; CAS Number 3483-12-3 Sodium deoxycholate Merck Cat# D6750; CAS Number 302-95-4 Tween 20 Sigma-Aldrich Cat# P7949; CAS Number 9005-64-5 Triton X-100 Merck Cat# T9284; CAS Number 9002-93-1 ATP Merck Cat# A9187; CAS Number 74804-12-9 DMSO Merck Cat# D2650; CAS Number 67-68-5 PMSF Sigma-Aldrich Cat# P7626; CAS Number 329-98-6 Imidazole Bio Basic Cat# IB0277; CAS Number 288-32-4 Lysozyme Sigma-Aldrich Cat# L4919; CAS Number 12650-88-3 Proteinase K Bio Basic Cat# PB0451; CAS Number 39450-01-6 L-Glutathione reduced Merck Cat# G6529; CAS Number 70-18-8 N, N′Methylenebisacrylamide Sigma-Aldrich Cat# M7279; CAS Number 110-26-9 Puromycin Sigma-Aldrich Cat# P8833; CAS Number 58-58-2 HEPES Sigma-Aldrich Cat# H3375; CAS Number 7365-45-9 EDTA Merck Cat# 324503; CAS Number 6381-92-6 EGTA Merck Cat# 324626; CAS Number 67-42-5 Na2HPO4 Merck Cat# 106559; CAS Number 7558-79-4 KCl Merck Cat# 104936; CAS Number 7447-40-7 14 EMBO Molecular Medicine © The Author(s) Reagent/resource Reference or source Identifier or Catalogue Number NaCl Merck Cat# 106406; CAS Number 7647-14-5 MgCl2 Sigma-Aldrich Cat# 814733; CAS Number: 7786-30-3 BSA Sigma-Aldrich Cat# SRE0098; CAS Number: 9048-46-8 2-Mercaptoethanol Sigma-Aldrich Cat# 63689; CAS Number: 60-24-2 SDS Sigma-Aldrich Cat# L3771; CAS Number: 151-21-3 Tris(hydroxymethyl) aminomethane Millipore Cat# 108382; CAS Number: 77-86-1 Glycine Millipore Cat# 3570; CAS Number: 56-40-6 Sucrose Sigma-Aldrich Cat# S9378; CAS Number: 57-50-1 Methanol Merck CAS Number: 67-56-1 CCCP Sigma-Aldrich Cat# C2759; CAS Number 555-60-2 BrdU Sigma-Aldrich Cat# B5002; CAS Number 59-14-3 IPTG Sigma-Aldrich Cat# I6758; CAS Number 367-93-1 MG132 Merck Cat# 474787; CAS Number 133407-82-6 Uridine Sigma-Aldrich Cat# U3003; CAS Number 58-96-8 Verapamil hydrochloride Merck CAS Number 152-11-4 Tazobactam Merck CAS Number 89786- 04-9 Clavulanic acid Merck CAS Number 58001- 44-8 D (-)-Mannitol Pure Merck Cat# M9647; CAS Number 69-65-8 Ampicillin Bio Basic Cat# AB0028 Kanamycin Bio Basic Cat# KB0286 Hygromycin B Sigma-Aldrich Cat# H7772 Penicillin-StreptomycinAmphotericin B Solution Biological industries Cat# 03-033-1B KH2PO4 Merck Cat# 104873; CAS Number 7778-77-0 Ethanol Merck Cat# 100983; CAS Number 64-17-5 Glycerol Merck Cat# 107051; CAS Number 56-81-5 TEMED Bio-Rad Cat# 1610801 Ammonium Persulfate Sigma-Aldrich Cat# A3678; CAS Number 7727-54-0 Coomassie brilliant blue-R-250 Bio-Rad Cat# 1610400 Ethidium bromide (EtBr) Sigma-Aldrich Cat# E7637 Luria Bertani Broth, Miller Himedia Cat# M1245 Reagent/resource Reference or source Identifier or Catalogue Number Luria Bertani Agar, Miller Himedia Cat# M1151 Skim milk powder Himedia Cat# GRM1254 Agarose Sigma-Aldrich Cat# A9539 Foetal bovine Serum Thermo Fisher Scientific Cat# 10082147 Advanced DMEM Thermo Fisher Scientific Cat# 12491-023 DMEM/F-12 medium Thermo Fisher Scientific Cat# 11320033 Horse serum Thermo Fisher Scientific Cat# 16050130 OptiMem reduced serum medium Thermo Fisher Scientific Cat# 31985-088 Complete Protease Cocktail inhibitor Roche Cat# 116974980 BL21 (DE3) competent cells Thermo Fisher Scientific Cat# EC0114 Poly-Prep Chromatography column Bio-Rad Cat# 73101550 Mounting medium with DAPI Vector Laboratories Cat# H-1200 Nhe1-HF New England BioLabs Cat# R3131 Not1-HF New England BioLabs Cat# R3189 Spe1-HF New England BioLabs Cat# R3133 XhoI New England BioLabs Cat# R0146 BamHI-HF New England BioLabs Cat# R3136 HindIII-HF New England BioLabs Cat# R3104 Glutathione SSepharose beads Cytiva Cat# 17-5279-02 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 Sepharose CL-6B Sigma-Aldrich Cat# CL6B200 3X Flag peptide Sigma-Aldrich Cat# F4799 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 LongAmp Taq DNA polymerase New England Biolabs Cat# M0323L Immobilon Western Chemiluminescent HRP substrate Merck Cat# WBKLS0500 10Χ PBS Sartorius Cat# 02-023-5 A Trypsin (2.5%) Thermo Fisher Scientific Cat# 15090046 L-Glutamine solution Biological industries Cat# 03-020-1B Prestained Low Molecular weight markers Bio-Rad Cat# 1610374 Wide range DNA marker Sigma-Aldrich Cat# D7058 Nitrocellulose membrane Bio-Rad Cat# 1620115 Streptavidin Agarose Millipore Cat# 69203 T7 Quick-coupled Transcription/ Translation system Promega Cat# L2080 © The Author(s) EMBO Molecular Medicine 15 Reagent/resource Reference or source Identifier or Catalogue Number Software GraphPad Prism 9 https://www.graphpad. .. com/ Other HOOK-Biotin-PEG2Amine Labelling Kit G-Biosciences Cat# BS-16 Mitochondrial DNA isolation kit BioVision Inc. Cat# K280-50 QuikChange II XL SiteDirected Mutagenesis Kit Agilent Cat# 200522 Qubit:Article Title: Consistent temperature-dependent effects on multiple displacement amplification. Article Snippet: Multiple displacement amplification (MDA) is widely used in the sample preparation steps of sequencing, but the amplification bias remains a major obstacle to its application.. While elevating reaction temperature has been reported to improve MDA performance, the strand thermodynamics across various temperatures remain poorly understood.. Through a comparative analysis of the sequencing data generated from MDA reactions performed at different temperatures, we characterized the temperature-dependent patterns of genomic bias and found that performing MDA at 38C optimizes coverage uniformity for complex human genomic samples. Sensitivity:Article Title: Consistent temperature-dependent effects on multiple displacement amplification. Article Snippet: Multiple displacement amplification (MDA) is widely used in the sample preparation steps of sequencing, but the amplification bias remains a major obstacle to its application.. While elevating reaction temperature has been reported to improve MDA performance, the strand thermodynamics across various temperatures remain poorly understood.. Through a comparative analysis of the sequencing data generated from MDA reactions performed at different temperatures, we characterized the temperature-dependent patterns of genomic bias and found that performing MDA at 38C optimizes coverage uniformity for complex human genomic samples. Isolation:Article Title: Highly multiplexed mammalian metabolic engineering with a shotgun approach. Article Snippet: .. gDNA was isolated from 3–6 million cells using either the Purification:Article Title: Highly multiplexed mammalian metabolic engineering with a shotgun approach. Article Snippet: .. gDNA was isolated from 3–6 million cells using either the Article Title: Clinical Evaluation of a Stool‐Based Molecular Point‐of‐Care Test Kit for Noninvasive Detection of Helicobacter pylori Infection and Clarithromycin Resistance Article Snippet: .. For stool, DNA was extracted from 100 μL of stool collection liquid with the Whole-Genome Sequencing:Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using Whole Complete genome sequencing:Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using Analysis:Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using Cell Culture:Article Title: Helicobacter pylori in Russian children: a multidimensional cross-sectional study Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-65019-0 DNA extraction, whole-genome sequencing (WGS) and bioinformatics analysis The genomic DNA was extracted from the gastric homogenized samples using D-Cells kit (Biolabmix, Russia), and cultured H. pylori isolates using Polymerase Chain Reaction:Article Title: Clinical Evaluation of a Stool‐Based Molecular Point‐of‐Care Test Kit for Noninvasive Detection of Helicobacter pylori Infection and Clarithromycin Resistance Article Snippet: .. For stool, DNA was extracted from 100 μL of stool collection liquid with the |
