Journal: International Journal of Molecular Sciences
Article Title: Transcriptional Analysis of Hair Follicle-Derived Keratinocytes from Donors with Atopic Dermatitis Reveals Enhanced Induction of IL32 Gene by IFN-γ
doi: 10.3390/ijms14023215
Figure Lengend Snippet: Genes showing differential expression between FDKs from donors with atopic dermatitis (AD) and from non-atopic controls (Non-AD) by microarray analysis, as determined by comparison analysis using a permutation test. The log 2 ratio (the Cy5 to Cy3 fluorescence intensity using the same Cy3-labelled probe, consisting of a mixture of NHEK extracts and Universal Human Reference RNA, as a reference) was used as a expression level. For comparative studies of gene expression between AD-FDKs and Non-AD-FDKs, a permutation test was performed (number of permutations: 500) and 827 genes were extracted. In this figure, 11 genes that exhibit a difference of at least 2-fold and meet the criterion p < 0.05 are visualized as the tree after hierarchical clustering (Distance; pearson uncentered, Linkage; complete).
Article Snippet: Probes with poor-quality signals (signal/noise < 1.2) and those with null data at a frequency of more than 35% in all samples were filtered out using the microarray data analysis software Expressionist (GeneData AG, Basel, Switzerland); after this procedure, 13,722 probes remained.
Techniques: Quantitative Proteomics, Microarray, Comparison, Fluorescence, Expressing, Gene Expression