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rhodamine phalloidin  (Cytoskeleton Inc)


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    Cytoskeleton Inc rhodamine phalloidin
    IL-17C stimulation promotes human intestinal microvascular endothelial cells (HIMEC) angiogenesis. ( A ) Representative bands of RT-PCR and quantitative analyses of two receptors of IL-17C, IL-17RA ( A , left ), and IL-17RE ( A , right ) in HIMECs treated with vehicle control or IL-17C (100 ng/mL, 3 h) are shown ( n = 3). GAPDH was used for the loading control. NS, not significant. ( B ) HIMECs were seeded into polymerized Matrigel and treated with IL-17C (100 ng/mL, 4 h) for in vitro tube formation assays ( B , left ). Scale bar, 100 μm. Total tube length of photos was measured using ImageJ v.1.47 software ( n = 6) ( B , right ). Error bars, SD. ** p < 0.01 vs. the control group. ( C ) The mouse aortic ring assay was conducted to investigate ex vivo angiogenic activity of IL-17C (100 ng/mL, 14 d) ( C , top ). Scale bar, 50 μm. For quantification, total sprouted tube vessels of 3 independent photos from each group were measured using ImageJ v.1.47 software ( C , bottom ). Error bars, SEM ( n = 3). *** p < 0.001 vs. the control group. ( D ) For the wound healing assays, cells were seeded in a culture plate, incubated with culture media with/without IL-17C (100 ng/mL), and scratched to create wounds. Photos were taken 24 h after wound creation ( D , left ). Scale bar, 50 μm. A view of representative fields from images of the wound healing assays, analyzed using ImageJ v.1.47 software ( D , right ). Error bars, SD ( n = 6). ** p < 0.01 vs. the control group. ( E ) F-actin organization in endothelial cells determined via <t>rhodamine-phalloidin</t> staining ( n = 3). Scale bar, 50 μm. ( F ) PCR microarray was performed to investigate expression levels of angiogenesis-related genes by IL-17C (100 ng/mL, 3 h) treatment in HIMECs ( n = 1). ( G ) The mRNA levels of CXCL3, IL-8, MMP-1, and MMP-10 were analyzed by qPCR at 3 h post IL-17C treatment. Error bars, SD ( n = 3). ** p < 0.01 and *** p < 0.001 vs. the control group.
    Rhodamine Phalloidin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 97/100, based on 1288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+dara+analysis+tool/Rhodamine+Phalloidin/pmc07348989-98-45-48
    Average 97 stars, based on 1288 article reviews
    rhodamine phalloidin - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "miR-23a-3p is a Key Regulator of IL-17C-Induced Tumor Angiogenesis in Colorectal Cancer"

    Article Title: miR-23a-3p is a Key Regulator of IL-17C-Induced Tumor Angiogenesis in Colorectal Cancer

    Journal: Cells

    doi: 10.3390/cells9061363

    IL-17C stimulation promotes human intestinal microvascular endothelial cells (HIMEC) angiogenesis. ( A ) Representative bands of RT-PCR and quantitative analyses of two receptors of IL-17C, IL-17RA ( A , left ), and IL-17RE ( A , right ) in HIMECs treated with vehicle control or IL-17C (100 ng/mL, 3 h) are shown ( n = 3). GAPDH was used for the loading control. NS, not significant. ( B ) HIMECs were seeded into polymerized Matrigel and treated with IL-17C (100 ng/mL, 4 h) for in vitro tube formation assays ( B , left ). Scale bar, 100 μm. Total tube length of photos was measured using ImageJ v.1.47 software ( n = 6) ( B , right ). Error bars, SD. ** p < 0.01 vs. the control group. ( C ) The mouse aortic ring assay was conducted to investigate ex vivo angiogenic activity of IL-17C (100 ng/mL, 14 d) ( C , top ). Scale bar, 50 μm. For quantification, total sprouted tube vessels of 3 independent photos from each group were measured using ImageJ v.1.47 software ( C , bottom ). Error bars, SEM ( n = 3). *** p < 0.001 vs. the control group. ( D ) For the wound healing assays, cells were seeded in a culture plate, incubated with culture media with/without IL-17C (100 ng/mL), and scratched to create wounds. Photos were taken 24 h after wound creation ( D , left ). Scale bar, 50 μm. A view of representative fields from images of the wound healing assays, analyzed using ImageJ v.1.47 software ( D , right ). Error bars, SD ( n = 6). ** p < 0.01 vs. the control group. ( E ) F-actin organization in endothelial cells determined via rhodamine-phalloidin staining ( n = 3). Scale bar, 50 μm. ( F ) PCR microarray was performed to investigate expression levels of angiogenesis-related genes by IL-17C (100 ng/mL, 3 h) treatment in HIMECs ( n = 1). ( G ) The mRNA levels of CXCL3, IL-8, MMP-1, and MMP-10 were analyzed by qPCR at 3 h post IL-17C treatment. Error bars, SD ( n = 3). ** p < 0.01 and *** p < 0.001 vs. the control group.
    Figure Legend Snippet: IL-17C stimulation promotes human intestinal microvascular endothelial cells (HIMEC) angiogenesis. ( A ) Representative bands of RT-PCR and quantitative analyses of two receptors of IL-17C, IL-17RA ( A , left ), and IL-17RE ( A , right ) in HIMECs treated with vehicle control or IL-17C (100 ng/mL, 3 h) are shown ( n = 3). GAPDH was used for the loading control. NS, not significant. ( B ) HIMECs were seeded into polymerized Matrigel and treated with IL-17C (100 ng/mL, 4 h) for in vitro tube formation assays ( B , left ). Scale bar, 100 μm. Total tube length of photos was measured using ImageJ v.1.47 software ( n = 6) ( B , right ). Error bars, SD. ** p < 0.01 vs. the control group. ( C ) The mouse aortic ring assay was conducted to investigate ex vivo angiogenic activity of IL-17C (100 ng/mL, 14 d) ( C , top ). Scale bar, 50 μm. For quantification, total sprouted tube vessels of 3 independent photos from each group were measured using ImageJ v.1.47 software ( C , bottom ). Error bars, SEM ( n = 3). *** p < 0.001 vs. the control group. ( D ) For the wound healing assays, cells were seeded in a culture plate, incubated with culture media with/without IL-17C (100 ng/mL), and scratched to create wounds. Photos were taken 24 h after wound creation ( D , left ). Scale bar, 50 μm. A view of representative fields from images of the wound healing assays, analyzed using ImageJ v.1.47 software ( D , right ). Error bars, SD ( n = 6). ** p < 0.01 vs. the control group. ( E ) F-actin organization in endothelial cells determined via rhodamine-phalloidin staining ( n = 3). Scale bar, 50 μm. ( F ) PCR microarray was performed to investigate expression levels of angiogenesis-related genes by IL-17C (100 ng/mL, 3 h) treatment in HIMECs ( n = 1). ( G ) The mRNA levels of CXCL3, IL-8, MMP-1, and MMP-10 were analyzed by qPCR at 3 h post IL-17C treatment. Error bars, SD ( n = 3). ** p < 0.01 and *** p < 0.001 vs. the control group.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, In Vitro, Software, Aortic Ring Assay, Ex Vivo, Activity Assay, Incubation, Staining, Microarray, Expressing

    Related Articles

    Labeling:

    Article Title: Fabrication of biomimetic topological micropattern arrays to induce anisotropic/isotropic orientation of fibroblast
    Article Snippet: .. 100 μL 100 nM rhodamine labeled phalloidin working solution (Cytoskeleton) was added to fully cover the cells on the micropattern array chips, and the samples were stained at room temperature for 45 min. After staining, the phalloidin working solution was removed, followed by washing three times with PBS. .. • 100 μL 10 μg/mL DAPI staining working solution (Biosharp) was then added, and the samples were stained at room temperature for 5 min.

    Staining:

    Article Title: Fabrication of biomimetic topological micropattern arrays to induce anisotropic/isotropic orientation of fibroblast
    Article Snippet: .. 100 μL 100 nM rhodamine labeled phalloidin working solution (Cytoskeleton) was added to fully cover the cells on the micropattern array chips, and the samples were stained at room temperature for 45 min. After staining, the phalloidin working solution was removed, followed by washing three times with PBS. .. • 100 μL 10 μg/mL DAPI staining working solution (Biosharp) was then added, and the samples were stained at room temperature for 5 min.



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