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    Thermo Fisher -provided human canine microarray comparisons
    Confirmation of cDNA-Array data by quantitative RT-PCT
    Provided Human Canine Microarray Comparisons, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+comparisons/IL-12%2FIL-23+p40+Canine+ProcartaPlex+Simplex+Kit/pmc02994823-223-19-16
    Average 90 stars, based on 1 article reviews
    -provided human canine microarray comparisons - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Metastatic canine mammary carcinomas can be identified by a gene expression profile that partly overlaps with human breast cancer profiles"

    Article Title: Metastatic canine mammary carcinomas can be identified by a gene expression profile that partly overlaps with human breast cancer profiles

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-10-618

    Confirmation of cDNA-Array data by quantitative RT-PCT
    Figure Legend Snippet: Confirmation of cDNA-Array data by quantitative RT-PCT

    Techniques Used: Microarray

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    other:

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    Article Snippet: The PGF 2α kit was purchased from Assay Designs and the IL-23p19 kit was purchased from eBioscience.

    Article Title: Evaluation of cutaneous and circulating (serum and exosomes) levels of chemokines (CCL17, CCL22, CCL27 and CCL28) in atopic dogs and their correlation with severity of the disease.
    Article Snippet: Materials and methods – Skin biopsies and blood samples were taken from 12 privately owned atopic [lesional (AD-L) and nonlesional (AD-NL) skin] and 12 privately owned healthy dogs.. Circulating exosomes were extracted from the serum.. Cutaneous and exosomal mRNA levels of CCL17, CCL22, CCL27 and CCL28 were quantified using quantitative real-time PCR.

    Article Title: Neospora caninum: a new class of biopharmaceuticals in the therapeutic arsenal against cancer
    Article Snippet: Cytokine production in splenocyte culture supernatants and/or tumor dissociation supernatants were evaluated by ELISA with specific kits: IL-1β, IL-6, IL-12p40, IL-18 and transforming growth factor (TGF)-β (eBioscience), CCL2 and VEGF (Invitrogen), IL-15 and PDL1 (R&D systems).

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    Microarray:

    Article Title: Comparison of regional gene expression differences in the brains of the domestic dog and human
    Article Snippet: .. Note that a comprehensive Affymetrix short oligonucleotide canine microarray has also just been described,[ , ] as have two small targeted cardiovascular arrays [ , ]. ..

    Article Title: Metastatic canine mammary carcinomas can be identified by a gene expression profile that partly overlaps with human breast cancer profiles
    Article Snippet: .. To supplement the gene annotations of the differentially expressed genes with functional information, BLAST search and Affymetrix-provided human to canine microarray comparisons were used to map canine genes to their human equivalents as has been shown before [ ]. ..

    Gene Expression:

    Article Title: Lack of functional alpha-lactalbumin prevents involution in Cape fur seals and identifies the protein as an apoptotic milk factor in mammary gland involution
    Article Snippet: .. Affymetrix canine arrays were used to examine the pattern of gene expression in mammary tissue from Cape fur seals ( Arctocephalus pusillus ) during pregnancy, on-shore lactation and off-shore lactation. ..

    Functional Assay:

    Article Title: Metastatic canine mammary carcinomas can be identified by a gene expression profile that partly overlaps with human breast cancer profiles
    Article Snippet: .. To supplement the gene annotations of the differentially expressed genes with functional information, BLAST search and Affymetrix-provided human to canine microarray comparisons were used to map canine genes to their human equivalents as has been shown before [ ]. ..



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    Image Search Results


    Confirmation of cDNA-Array data by quantitative RT-PCT

    Journal: BMC Cancer

    Article Title: Metastatic canine mammary carcinomas can be identified by a gene expression profile that partly overlaps with human breast cancer profiles

    doi: 10.1186/1471-2407-10-618

    Figure Lengend Snippet: Confirmation of cDNA-Array data by quantitative RT-PCT

    Article Snippet: To supplement the gene annotations of the differentially expressed genes with functional information, BLAST search and Affymetrix-provided human to canine microarray comparisons were used to map canine genes to their human equivalents as has been shown before [ ].

    Techniques: Microarray

    Figure 1. Study design. Carotid plaques (n=34) from asymptomatic and symptomatic patients were sectioned at 2 levels and 3 cores drilled from each level (A) to construct 2 tissue microarray (TMA) blocks with 204 cores in total (B). Based on symptomatic vs asymptom- atic microarray comparisons generated for n=127 plaques (C), highly upregulated genes were chosen for the study (D; in red). Several genes already known to be associated with atherosclerosis were also included (D; in black). TMAs were stained by immunohistochemistry (IHC) with antibodies toward human proteins of interest, and tissue sections were scored (0, 1, 2, or 3) for semiquantitative grading of staining intensity (E). Significantly differentially expressed proteins were validated further by IHC on individual plaques (F).

    Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

    Article Title: Profiling of Atherosclerotic Lesions by Gene and Tissue Microarrays Reveals PCSK6 as a Novel Protease in Unstable Carotid Atherosclerosis

    doi: 10.1161/atvbaha.113.301743

    Figure Lengend Snippet: Figure 1. Study design. Carotid plaques (n=34) from asymptomatic and symptomatic patients were sectioned at 2 levels and 3 cores drilled from each level (A) to construct 2 tissue microarray (TMA) blocks with 204 cores in total (B). Based on symptomatic vs asymptom- atic microarray comparisons generated for n=127 plaques (C), highly upregulated genes were chosen for the study (D; in red). Several genes already known to be associated with atherosclerosis were also included (D; in black). TMAs were stained by immunohistochemistry (IHC) with antibodies toward human proteins of interest, and tissue sections were scored (0, 1, 2, or 3) for semiquantitative grading of staining intensity (E). Significantly differentially expressed proteins were validated further by IHC on individual plaques (F).

    Article Snippet: From the list of highly upregulated genes obtained by microarray comparisons between symptomatic and asymptomatic carotid lesions, candidates with relatively restricted tissue distribution in humans were chosen for further analysis (based on the Human Protein Atlas).

    Techniques: Construct, Microarray, Generated, Staining, Immunohistochemistry

    Figure 4. PCSK6 is the most significantly upregulated proprotein convertase (PC) in symptomatic carotid plaques. Analy- ses of n=127 microarray profiles between carotid plaques (CP) and normal controls (iliac arteries [IA]) show significant down- regulation of Furin and PCSK5 in plaque tissue, upregulation of PCSK6 and PCSK7, whereas PCSK9 and PCSK2 did not exhibit significant variation in expression (A). Posi- tive correlation was found between expres- sion of PCSK6 and PCSK9, PCSK2 and PCSK7, whereas negative was observed with PCSK5 (B). Several PCSK6 isoforms recognized by different Affymetrix probes were significantly upregulated in plaques compared with controls, but most highly the isoform coding for secreted protein (C). This isoform was the only one with signifi- cantly higher expression in comparison between symptomatic (s) and asymptom- atic (as) plaques (D). Results were con- firmed by quantitative real-time polymerase chain reaction (qRT-PCR) analyses for expression of secreted PCSK6 isoform in a separate cohort of n=233 carotid plaques (E and F). Micorarray data expressed as log2 value and qRT-PCR data as fold change compared with control. Data in A, C, and D show mean with SD, whereas median with interquartile range is depicted in E and F. ER indicates endoplasmatic reticulum.

    Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

    Article Title: Profiling of Atherosclerotic Lesions by Gene and Tissue Microarrays Reveals PCSK6 as a Novel Protease in Unstable Carotid Atherosclerosis

    doi: 10.1161/atvbaha.113.301743

    Figure Lengend Snippet: Figure 4. PCSK6 is the most significantly upregulated proprotein convertase (PC) in symptomatic carotid plaques. Analy- ses of n=127 microarray profiles between carotid plaques (CP) and normal controls (iliac arteries [IA]) show significant down- regulation of Furin and PCSK5 in plaque tissue, upregulation of PCSK6 and PCSK7, whereas PCSK9 and PCSK2 did not exhibit significant variation in expression (A). Posi- tive correlation was found between expres- sion of PCSK6 and PCSK9, PCSK2 and PCSK7, whereas negative was observed with PCSK5 (B). Several PCSK6 isoforms recognized by different Affymetrix probes were significantly upregulated in plaques compared with controls, but most highly the isoform coding for secreted protein (C). This isoform was the only one with signifi- cantly higher expression in comparison between symptomatic (s) and asymptom- atic (as) plaques (D). Results were con- firmed by quantitative real-time polymerase chain reaction (qRT-PCR) analyses for expression of secreted PCSK6 isoform in a separate cohort of n=233 carotid plaques (E and F). Micorarray data expressed as log2 value and qRT-PCR data as fold change compared with control. Data in A, C, and D show mean with SD, whereas median with interquartile range is depicted in E and F. ER indicates endoplasmatic reticulum.

    Article Snippet: From the list of highly upregulated genes obtained by microarray comparisons between symptomatic and asymptomatic carotid lesions, candidates with relatively restricted tissue distribution in humans were chosen for further analysis (based on the Human Protein Atlas).

    Techniques: Microarray, Expressing, Comparison, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control