Review




Structured Review

CombiMatrix microarray imager
Fluorescence detection of rehybridization by target oligonucleotide to probes on the <t>microarray</t> used in following stripping at 95 °C for 1 h. Different concentrations (0, 2, 20, or 200 pM) of biotinylated target oligonucleotide were incubated in individual chambers of a four-chamber hyb cap, and binding was detected using Cy5-SA. ( A ) Complementary 5′-aminated DNA probe immobilized on Ppy deposited using constant current from 10 to 520 nA for 1.0 s. (B) Same as (4A), but a complementary DNA probe with an 5′-aminated T-linker was immobilized on the Ppy. ( C ) Same as (4A), but a non-complementary DNA probe with a 5′-aminated T-linker was immobilized on the Ppy.
Microarray Imager, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+chip+combimatrix+12k+electrasense/microarray+imager/pmc03231185-62-4-9
Average 90 stars, based on 1 article reviews
microarray imager - by Bioz Stars, 2026-11
90/100 stars

Images

1) Product Images from "Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes"

Article Title: Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s100807371

Fluorescence detection of rehybridization by target oligonucleotide to probes on the microarray used in following stripping at 95 °C for 1 h. Different concentrations (0, 2, 20, or 200 pM) of biotinylated target oligonucleotide were incubated in individual chambers of a four-chamber hyb cap, and binding was detected using Cy5-SA. ( A ) Complementary 5′-aminated DNA probe immobilized on Ppy deposited using constant current from 10 to 520 nA for 1.0 s. (B) Same as (4A), but a complementary DNA probe with an 5′-aminated T-linker was immobilized on the Ppy. ( C ) Same as (4A), but a non-complementary DNA probe with a 5′-aminated T-linker was immobilized on the Ppy.
Figure Legend Snippet: Fluorescence detection of rehybridization by target oligonucleotide to probes on the microarray used in following stripping at 95 °C for 1 h. Different concentrations (0, 2, 20, or 200 pM) of biotinylated target oligonucleotide were incubated in individual chambers of a four-chamber hyb cap, and binding was detected using Cy5-SA. ( A ) Complementary 5′-aminated DNA probe immobilized on Ppy deposited using constant current from 10 to 520 nA for 1.0 s. (B) Same as (4A), but a complementary DNA probe with an 5′-aminated T-linker was immobilized on the Ppy. ( C ) Same as (4A), but a non-complementary DNA probe with a 5′-aminated T-linker was immobilized on the Ppy.

Techniques Used: Fluorescence, Microarray, Stripping Membranes, Incubation, Binding Assay

Effects on ECD of adding a 5′-aminated 20 T-linker to DNA probes and preheating the immobilized probes prior to hybridization. Polypyrrole was deposited at 30 nA, and 20 pM of biotinylated oligonucleotide was hybridized on the array. A second microarray was incubated in 2XPBST for 1 h at 95 °C and washed once in PBS prior to hybridization.
Figure Legend Snippet: Effects on ECD of adding a 5′-aminated 20 T-linker to DNA probes and preheating the immobilized probes prior to hybridization. Polypyrrole was deposited at 30 nA, and 20 pM of biotinylated oligonucleotide was hybridized on the array. A second microarray was incubated in 2XPBST for 1 h at 95 °C and washed once in PBS prior to hybridization.

Techniques Used: Hybridization, Microarray, Incubation

Related Articles

Microarray:

Article Title: Gene Expression Profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation
Article Snippet: After imaging, microarrays were stripped for subsequent re-hybridisation up to three times using the CombiMatrix CustomArray stripping kit (CombiMatrix Corporation, Mukilteo, USA) according to manufacturer's instructions. .. Microarray Imager (CombiMatrix Corporation, Mukilteo, USA) was then used to create spot intensity reports, generate gene ID mapping files and assign gene identification. .. Microarray slides were scanned using a fluorescent microarray scanner (GenePix 4000B, MDS Analytical Technologies, Toronto, Canada).

Article Title: Potential mechanisms underlying embryonic developmental toxicity caused by benzo[a]pyrene in Japanese medaka (Oryzias latipes).
Article Snippet: The medaka embryos incorporating BaP revealed typical teratogenic and developmental effects.. The gene expression analysis presented several genes and hub proteins involved in the developmental effects of BaP on medaka embryos.. Our findings suggest a potential mechanism of teratogenicity and developmental toxicity caused by exposure to BaP. a r t i c l e i n f o

Article Title: Nanosecond pulsed electric field incorporation technique to predict molecular mechanisms of teratogenicity and developmental toxicity of estradiol-17β on medaka embryos.
Article Snippet: Department of Creative Engineering, National Institute of Technology, Ariake College, 150 Higashi‐Hagio, Omuta, Fukuoka 836‐8585, Japan Graduate School of Agriculture, Ehime University, 3‐5‐7 Tarumi, Matsuyama 790‐8566, Japan Computer Science and Systems Engineering, Kyushu Institute of Technology, 680‐4 Kawazu, Iizuka, Fukuoka 820‐0067, Japan Mizuki Biotech, Co., Ltd., 1‐1 Hyakunenkouen, Kurume, Fukuoka 839‐0864, Japan Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3‐1‐100 Tsukide, Higashi‐ku, Kumamoto 862‐ 8502, Japan Correspondence Nobuaki Tominaga, 150 Higashi‐Hagio, Omuta, Fukuoka 836‐8585, Japan.. Email: tominaga@ariake‐nct.ac.jp Funding information Japan Society for the Promotion of Sciences, Japan; Grants‐in‐Aid for Scientific Research(B), Grant/Award Number: 16H02990

Article Title: Expression analysis of ginsenoside biosynthesis-related genes in methyl jasmonate-treated adventitious roots of Panax ginseng via DNA microarray analysis
Article Snippet: Many ginsenoside biosynthesis-related genes have been identified in Panax ginseng.. However, there is no report of the analysis of changes in gene expression induced methyl jasmonic acid (MeJA) using DNA microarray analysis.. To identify the genes related to ginsenoside biosynthesis, we harvested P. ginseng adventitious roots at 0, 24, 72 and 120 h after MeJA treatment.

Article Title: The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola
Article Snippet: Microarrays were scanned using ScanArray 4000 XL (PerkinElmer Life Sciences, Waltham, MA, USA). .. Data extracted with Microarray Imager (Combimatrix, Irvine, CA, USA) were normalized by median scaling before deposition in NCBI GEO database (ID: GSE42972). ..

Expressing:

Article Title: Potential mechanisms underlying embryonic developmental toxicity caused by benzo[a]pyrene in Japanese medaka (Oryzias latipes).
Article Snippet: The medaka embryos incorporating BaP revealed typical teratogenic and developmental effects.. The gene expression analysis presented several genes and hub proteins involved in the developmental effects of BaP on medaka embryos.. Our findings suggest a potential mechanism of teratogenicity and developmental toxicity caused by exposure to BaP. a r t i c l e i n f o

Article Title: Nanosecond pulsed electric field incorporation technique to predict molecular mechanisms of teratogenicity and developmental toxicity of estradiol-17β on medaka embryos.
Article Snippet: Department of Creative Engineering, National Institute of Technology, Ariake College, 150 Higashi‐Hagio, Omuta, Fukuoka 836‐8585, Japan Graduate School of Agriculture, Ehime University, 3‐5‐7 Tarumi, Matsuyama 790‐8566, Japan Computer Science and Systems Engineering, Kyushu Institute of Technology, 680‐4 Kawazu, Iizuka, Fukuoka 820‐0067, Japan Mizuki Biotech, Co., Ltd., 1‐1 Hyakunenkouen, Kurume, Fukuoka 839‐0864, Japan Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3‐1‐100 Tsukide, Higashi‐ku, Kumamoto 862‐ 8502, Japan Correspondence Nobuaki Tominaga, 150 Higashi‐Hagio, Omuta, Fukuoka 836‐8585, Japan.. Email: tominaga@ariake‐nct.ac.jp Funding information Japan Society for the Promotion of Sciences, Japan; Grants‐in‐Aid for Scientific Research(B), Grant/Award Number: 16H02990

Article Title: Transcriptional response of mysid crustacean, Americamysis bahia, is affected by subchronic exposure to nonylphenol.
Article Snippet: Nonylphenol (NP) has been classified as an endocrine-disrupting chemical.. In this study, we conducted mysid DNA microarray analysis with which has 2240 oligo DNA probes to observe differential gene expressions in mysid crustacean (Americamysis bahia) exposed to 1, 3, 10 and 30 μg/l of NP for 14 days.. As a result, we found 31, 27, 39 and 68 genes were differentially expressed in the respective concentrations.

Software:

Article Title: Expression analysis of ginsenoside biosynthesis-related genes in methyl jasmonate-treated adventitious roots of Panax ginseng via DNA microarray analysis
Article Snippet: Many ginsenoside biosynthesis-related genes have been identified in Panax ginseng.. However, there is no report of the analysis of changes in gene expression induced methyl jasmonic acid (MeJA) using DNA microarray analysis.. To identify the genes related to ginsenoside biosynthesis, we harvested P. ginseng adventitious roots at 0, 24, 72 and 120 h after MeJA treatment.



Similar Products

90
CombiMatrix microarray chip combimatrix 12k electrasense
Microarray Chip Combimatrix 12k Electrasense, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+chip+combimatrix+12k+electrasense/combimatrix+electrasenseh+microarray/pm27286805-44-41-45
Average 90 stars, based on 1 article reviews
microarray chip combimatrix 12k electrasense - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results