streptavidin conjugated alexa 647 (Thermo Fisher)
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streptavidin conjugated alexa 647
Streptavidin Conjugated Alexa 647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+blocking+solution/Streptavidin/ppr0055369-104-7-10
Average 99 stars, based on 1 article reviews
Streptavidin Conjugated Alexa 647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+blocking+solution/Streptavidin/ppr0055369-104-7-10
Average 99 stars, based on 1 article reviews
streptavidin conjugated alexa 647 - by Bioz Stars,
2026-10
99/100 stars
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Protein Concentration:Article Title: OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy Article Snippet: .. In some experiments, bio-AddAB was pre-incubated with other:Article Title: Interacting proteins of AMPK studied using TurboID proximity labeling technology Article Snippet: Dynabeads MyOne streptavidin C1 (cat. no. 65001), biotin (cat. no. B20656 ) and Article Title: A conserved mammalian mecciRNA, mecciATP6, regulates mitochondrial homeostasis through interaction with HNRNPA3 Article Snippet: For smFISH and immunofluorescence (IF) analyses, the colocalization ratio between smFISH/IF signals and mitochondria was quantified using the Coloc2 plugin in ImageJ. Article Title: Targeting Mettl8-Tcf1 axis promotes CD8 + T PEX differentiation and antitumor immunity Article Snippet: The DNA ends were blunted and labeled with biotin by Klenow DNA polymerase in the presence of dCTP, dGTP, dTTP, and biotin-14-dATP, followed by ligation using T4 DNA ligase (NEB). Transfection:Article Title: CircEif3c/miR-96–5p/PHF20L1/MEOX2 axis in perivascular preadipocyte exosomes mediates fibroblast dysfunction and vascular remodeling Article Snippet: Biotin-labeled miRNAs were transfected into cells using Lipofectamine 3000 (#L3000-001, Invitrogen, Thermo Fisher Scientific, USA), and cell lysates were subsequently collected. .. Cell lysates were collected 48 h after transfection and incubated with Incubation:Article Title: CircEif3c/miR-96–5p/PHF20L1/MEOX2 axis in perivascular preadipocyte exosomes mediates fibroblast dysfunction and vascular remodeling Article Snippet: Biotin-labeled miRNAs were transfected into cells using Lipofectamine 3000 (#L3000-001, Invitrogen, Thermo Fisher Scientific, USA), and cell lysates were subsequently collected. .. Cell lysates were collected 48 h after transfection and incubated with Article Title: HSF1 and YTHDC1 enhance DNA repair under heat stress by promoting repair-associated gene expression Article Snippet: The samples were washed three more times in PBS containing 0.1% Tween 20, incubated with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/ml, Beyotime, C1002) for 5 min, and sealed. .. In addition, cells were incubated with Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma Article Snippet: Samples were acquired on a BD LSRFortessa flow cytometer. .. Biotinylated circSMAD4 sense RNA (with antisense RNA as a negative control) was generated by in vitro transcription using Biotin-16-UTP (Beyotime, Cat# D7336), incubated with macrophage lysates, and captured using Magnetic Beads:Article Title: CircEif3c/miR-96–5p/PHF20L1/MEOX2 axis in perivascular preadipocyte exosomes mediates fibroblast dysfunction and vascular remodeling Article Snippet: Biotin-labeled miRNAs were transfected into cells using Lipofectamine 3000 (#L3000-001, Invitrogen, Thermo Fisher Scientific, USA), and cell lysates were subsequently collected. .. Cell lysates were collected 48 h after transfection and incubated with Centrifugation:Article Title: CircEif3c/miR-96–5p/PHF20L1/MEOX2 axis in perivascular preadipocyte exosomes mediates fibroblast dysfunction and vascular remodeling Article Snippet: Biotin-labeled miRNAs were transfected into cells using Lipofectamine 3000 (#L3000-001, Invitrogen, Thermo Fisher Scientific, USA), and cell lysates were subsequently collected. .. Cell lysates were collected 48 h after transfection and incubated with Cell-Signaling:Article Title: Divalent siRNA for prion disease Article Snippet: .. The assay uses antibodies EP1802Y (Abcam, ab52604) for capture and in-house biotinylated 8H4 (Abcam ab61409) for detection, followed by Negative Control:Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma Article Snippet: Samples were acquired on a BD LSRFortessa flow cytometer. .. Biotinylated circSMAD4 sense RNA (with antisense RNA as a negative control) was generated by in vitro transcription using Biotin-16-UTP (Beyotime, Cat# D7336), incubated with macrophage lysates, and captured using Generated:Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma Article Snippet: Samples were acquired on a BD LSRFortessa flow cytometer. .. Biotinylated circSMAD4 sense RNA (with antisense RNA as a negative control) was generated by in vitro transcription using Biotin-16-UTP (Beyotime, Cat# D7336), incubated with macrophage lysates, and captured using In Vitro:Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma Article Snippet: Samples were acquired on a BD LSRFortessa flow cytometer. .. Biotinylated circSMAD4 sense RNA (with antisense RNA as a negative control) was generated by in vitro transcription using Biotin-16-UTP (Beyotime, Cat# D7336), incubated with macrophage lysates, and captured using |
![Gsk3 negatively regulates mitotic effectors in Xenopus extracts. (A) Scheme of the protein <t>microarray</t> experiment to assess Gsk3-dependent polyubiquitination. Interphase egg extracts were supplemented with ubiquitin, pretreated with LiCl or NaCl (Control), and then applied on protein arrays containing ∼9,000 proteins. Polyubiquitination of arrayed proteins (PolyUb) was monitored using anti-polyubiquitin antibodies and immunofluorescence detection using an array scanner. Signal intensities were transformed and are displayed as heat-map. (B) Distribution plot of polyubiquitination changes of 3,873 in vitro polyubiquitinated proteins after Gsk3 inhibition by LiCl (Log2[Control/LiCl]). Arrays were treated with 30 mM LiCl or 30 mM NaCl (Control). LiCl reduced polyubiquitination of 864 proteins >1.8-fold. Some candidates, associated with cell cycle progression and selected for further validation are indicated in the plot. (C) Cluster of mitotic associated proteins that are significantly regulated by LiCl in B. The functional network was determined using String 9.1 and color-coded based on the fold change in the microarray (Dataset S1). (D) Western blots of indicated proteins from Xenopus eggs (naturally arrested in metaphase II) and released into interphase with the Ca2+ ionophore A23187 for 25 min, treated with LiCl, or mock treated (NaCl). β-cat, β-catenin; cycE, cyclin E; Interph., interphase; M. II, metaphase II; pH3, phosphorylated histone 3. (E) Western blots of stage VI oocytes in Prophase I and Progesterone-matured (Prog.) oocytes in metaphase II. Oocytes were injected (Inj.) with Morpholinos (MO) or mRNA as indicated. Co, control; dnWnt11, dominant negative Wnt11; pH3, phosphorylated histone 3; PPL, preprolactin (negative control).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6427/pmc04426427/pmc04426427__pnas.1423533112fig02.jpg)