hybridization solution (Cytiva Europe)
Cytiva Europe is a verified supplier 
Cytiva Europe manufactures this product 
93
Structured Review
Cytiva Europe
hybridization solution
Hybridization Solution, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+blocking+solution/Microarray+Hybridization+Solution+v2%2E0/pmc08974173-72-32-31
Average 93 stars, based on 116 article reviews
Hybridization Solution, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+blocking+solution/Microarray+Hybridization+Solution+v2%2E0/pmc08974173-72-32-31
Average 93 stars, based on 116 article reviews
hybridization solution - by Bioz Stars,
2026-10
93/100 stars
Images
Related Articles
Blocking Assay:Article Title: Comprehensive analysis of peripheral blood non-coding RNAs identifies a diagnostic panel for fungal infection after transplantation Article Snippet: .. 1ul of the cDNA is mixed with 5ul 10X of blocking reagent and 1ul 25X of separation buffer at 60°C for 30 minutes, and then be diluted by 25ul 2X of Hybridization:Article Title: Comprehensive analysis of peripheral blood non-coding RNAs identifies a diagnostic panel for fungal infection after transplantation Article Snippet: .. 1ul of the cDNA is mixed with 5ul 10X of blocking reagent and 1ul 25X of separation buffer at 60°C for 30 minutes, and then be diluted by 25ul 2X of Article Title: A chemical defense deters cannibalism in migratory locusts Article Snippet: .. Subsequently, each slide was covered with 130 μl Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of Article Title: Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription Article Snippet: .. From this, 4 μg of DNA was labeled with fluorescent Cy5 dye and used for the Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Article Title: Long noncoding intronic RNAs are differentially expressed in primary and metastatic pancreatic cancer Article Snippet: .. For each sample, cRNA targets were ressuspended in a final volume of 200 μl of 1× Article Title: A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera. Article Snippet: .. Each slide with tissue sections was covered with 130 μL Article Title: The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria Article Snippet: .. After sectioning, the samples were treated for 20 min at 4 °C with 4% paraformaldehyde dissolved in PBS, washed in PBS for 5 min, and then incubated in 0.2 M HCl for 10 min. After washing the samples two times for 2 min in PBS, the slides were transferred into Microarray:Article Title: A chemical defense deters cannibalism in migratory locusts Article Snippet: .. Subsequently, each slide was covered with 130 μl Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of Article Title: Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription Article Snippet: .. From this, 4 μg of DNA was labeled with fluorescent Cy5 dye and used for the Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Article Title: Long noncoding intronic RNAs are differentially expressed in primary and metastatic pancreatic cancer Article Snippet: .. For each sample, cRNA targets were ressuspended in a final volume of 200 μl of 1× Article Title: A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera. Article Snippet: .. Each slide with tissue sections was covered with 130 μL Article Title: The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria Article Snippet: .. After sectioning, the samples were treated for 20 min at 4 °C with 4% paraformaldehyde dissolved in PBS, washed in PBS for 5 min, and then incubated in 0.2 M HCl for 10 min. After washing the samples two times for 2 min in PBS, the slides were transferred into Labeling:Article Title: A chemical defense deters cannibalism in migratory locusts Article Snippet: .. Subsequently, each slide was covered with 130 μl Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of Article Title: Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription Article Snippet: .. From this, 4 μg of DNA was labeled with fluorescent Cy5 dye and used for the Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Article Title: A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera. Article Snippet: .. Each slide with tissue sections was covered with 130 μL Expressing:Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of Concentration Assay:Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Incubation:Article Title: Long noncoding intronic RNAs are differentially expressed in primary and metastatic pancreatic cancer Article Snippet: .. For each sample, cRNA targets were ressuspended in a final volume of 200 μl of 1× Article Title: The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria Article Snippet: .. After sectioning, the samples were treated for 20 min at 4 °C with 4% paraformaldehyde dissolved in PBS, washed in PBS for 5 min, and then incubated in 0.2 M HCl for 10 min. After washing the samples two times for 2 min in PBS, the slides were transferred into |
![Gsk3 negatively regulates mitotic effectors in Xenopus extracts. (A) Scheme of the protein <t>microarray</t> experiment to assess Gsk3-dependent polyubiquitination. Interphase egg extracts were supplemented with ubiquitin, pretreated with LiCl or NaCl (Control), and then applied on protein arrays containing ∼9,000 proteins. Polyubiquitination of arrayed proteins (PolyUb) was monitored using anti-polyubiquitin antibodies and immunofluorescence detection using an array scanner. Signal intensities were transformed and are displayed as heat-map. (B) Distribution plot of polyubiquitination changes of 3,873 in vitro polyubiquitinated proteins after Gsk3 inhibition by LiCl (Log2[Control/LiCl]). Arrays were treated with 30 mM LiCl or 30 mM NaCl (Control). LiCl reduced polyubiquitination of 864 proteins >1.8-fold. Some candidates, associated with cell cycle progression and selected for further validation are indicated in the plot. (C) Cluster of mitotic associated proteins that are significantly regulated by LiCl in B. The functional network was determined using String 9.1 and color-coded based on the fold change in the microarray (Dataset S1). (D) Western blots of indicated proteins from Xenopus eggs (naturally arrested in metaphase II) and released into interphase with the Ca2+ ionophore A23187 for 25 min, treated with LiCl, or mock treated (NaCl). β-cat, β-catenin; cycE, cyclin E; Interph., interphase; M. II, metaphase II; pH3, phosphorylated histone 3. (E) Western blots of stage VI oocytes in Prophase I and Progesterone-matured (Prog.) oocytes in metaphase II. Oocytes were injected (Inj.) with Morpholinos (MO) or mRNA as indicated. Co, control; dnWnt11, dominant negative Wnt11; pH3, phosphorylated histone 3; PPL, preprolactin (negative control).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6427/pmc04426427/pmc04426427__pnas.1423533112fig02.jpg)