bovine albumin fraction v solution thermo fisher scientific (Thermo Fisher)
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Bovine Albumin Fraction V Solution Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Protein Concentration:Article Title: Title Pending 15701 Article Snippet: .. The protein concentration of each extract was determined using the Pierce Detergent Compatible Bradford Assay with bovine Bradford Assay:Article Title: Title Pending 15701 Article Snippet: .. The protein concentration of each extract was determined using the Pierce Detergent Compatible Bradford Assay with bovine Conjugation Assay:Article Title: Epstein-Barr virus orchestrates spatial reorganization and immunomodulation in the classic Hodgkin lymphoma tumor microenvironment Article Snippet: Maxpar X8 Multimetal Labeling Kit (Fluidigm, 201300) and Ionpath Conjugation Kits (Ionpath, 600XXX) were utilized. .. Briefly, 100 μg of bovine Incubation:Article Title: Epstein-Barr virus orchestrates spatial reorganization and immunomodulation in the classic Hodgkin lymphoma tumor microenvironment Article Snippet: Maxpar X8 Multimetal Labeling Kit (Fluidigm, 201300) and Ionpath Conjugation Kits (Ionpath, 600XXX) were utilized. .. Briefly, 100 μg of bovine Article Title: Natural senolytic activity of Rhodiola rosea extract alleviates age-associated phenotypes via paraptosis Article Snippet: The stained sections were visualized using a BZ-9000 microscope (Keyence Corporation). .. For immunofluorescence staining, paraffin sections were deparaffinized and subjected to antigen retrieval by microwave treatment in 10 mM Tris-EDTA buffer (pH 9.0) for 10 min. After blocking with bovine Saline:Article Title: The in-vitro effect of bovine milk, and plant-based alternatives on oral microorganisms and biofilms. Article Snippet: .. Wells of two 24-well plates were coated with 100 μL of test substances or 0.9% w/v NaCl for 15 min. Then 50 μL of Suspension:Article Title: The in-vitro effect of bovine milk, and plant-based alternatives on oral microorganisms and biofilms. Article Snippet: .. Wells of two 24-well plates were coated with 100 μL of test substances or 0.9% w/v NaCl for 15 min. Then 50 μL of Labeling:Article Title: Methods for generating surface coatings via self-assembly of silk fibroin and silk fibroin-like macromolecules Article Snippet: .. First, non-silk fibroin proteins, including other:Article Title: Low Density Neutrophil Heterogeneity and Spleen Tyrosine Kinase as a Therapeutic Target in Sepsis. Article Snippet: Following the overnight incubation period, plates were washed five times with PBS and blocking was performed with 5% Albumin Bovine, Immunofluorescence:Article Title: Natural senolytic activity of Rhodiola rosea extract alleviates age-associated phenotypes via paraptosis Article Snippet: The stained sections were visualized using a BZ-9000 microscope (Keyence Corporation). .. For immunofluorescence staining, paraffin sections were deparaffinized and subjected to antigen retrieval by microwave treatment in 10 mM Tris-EDTA buffer (pH 9.0) for 10 min. After blocking with bovine Staining:Article Title: Natural senolytic activity of Rhodiola rosea extract alleviates age-associated phenotypes via paraptosis Article Snippet: The stained sections were visualized using a BZ-9000 microscope (Keyence Corporation). .. For immunofluorescence staining, paraffin sections were deparaffinized and subjected to antigen retrieval by microwave treatment in 10 mM Tris-EDTA buffer (pH 9.0) for 10 min. After blocking with bovine Blocking Assay:Article Title: Natural senolytic activity of Rhodiola rosea extract alleviates age-associated phenotypes via paraptosis Article Snippet: The stained sections were visualized using a BZ-9000 microscope (Keyence Corporation). .. For immunofluorescence staining, paraffin sections were deparaffinized and subjected to antigen retrieval by microwave treatment in 10 mM Tris-EDTA buffer (pH 9.0) for 10 min. After blocking with bovine |
![Gsk3 negatively regulates mitotic effectors in Xenopus extracts. (A) Scheme of the protein <t>microarray</t> experiment to assess Gsk3-dependent polyubiquitination. Interphase egg extracts were supplemented with ubiquitin, pretreated with LiCl or NaCl (Control), and then applied on protein arrays containing ∼9,000 proteins. Polyubiquitination of arrayed proteins (PolyUb) was monitored using anti-polyubiquitin antibodies and immunofluorescence detection using an array scanner. Signal intensities were transformed and are displayed as heat-map. (B) Distribution plot of polyubiquitination changes of 3,873 in vitro polyubiquitinated proteins after Gsk3 inhibition by LiCl (Log2[Control/LiCl]). Arrays were treated with 30 mM LiCl or 30 mM NaCl (Control). LiCl reduced polyubiquitination of 864 proteins >1.8-fold. Some candidates, associated with cell cycle progression and selected for further validation are indicated in the plot. (C) Cluster of mitotic associated proteins that are significantly regulated by LiCl in B. The functional network was determined using String 9.1 and color-coded based on the fold change in the microarray (Dataset S1). (D) Western blots of indicated proteins from Xenopus eggs (naturally arrested in metaphase II) and released into interphase with the Ca2+ ionophore A23187 for 25 min, treated with LiCl, or mock treated (NaCl). β-cat, β-catenin; cycE, cyclin E; Interph., interphase; M. II, metaphase II; pH3, phosphorylated histone 3. (E) Western blots of stage VI oocytes in Prophase I and Progesterone-matured (Prog.) oocytes in metaphase II. Oocytes were injected (Inj.) with Morpholinos (MO) or mRNA as indicated. Co, control; dnWnt11, dominant negative Wnt11; pH3, phosphorylated histone 3; PPL, preprolactin (negative control).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6427/pmc04426427/pmc04426427__pnas.1423533112fig02.jpg)