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human mirna onearray ® mirna profiling chip  (Phalanx Biotech)

 
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    Structured Review

    Phalanx Biotech human mirna onearray ® mirna profiling chip
    A. A schematic representation of the procedure for <t>miRNA</t> selection. Differential expressions of miRNAs in osthole-treated cells versus vehicle-treated cells were analyzed with a <t>OneArray</t> ® miRNA profiling chip. B. Treatment of Du145 cells with osthole for 6 h. miR-146a, miR-22-3p, and miR-23a-3p expressions were detected by a quantitative PCR. C. Upper panel, Du145 cells were transfected with an miR-23a-3p mimic or mimic control for 24 h followed by osthole (60 μM) treatment for an additional 24 h. E-cadherin expression levels were determined by a Western blot analysis. Quantitative E-cadherin protein levels were adjusted to the β-actin protein level. Lower panel, Relative luciferase activities of DU145 cells co-transfected with an E-cadherin luciferase 3′UTR reporter vector and miR-23a-3p mimic or mimic control for 24 h. Values are presented as the mean ± SE of three independent experiments. * p < 0.05, compared to the control groups. D. DU145 cells were transfected with an miR-23a-3p mimic or mimic control for 24 h followed by osthole (60 μM) treatment for an additional 24 h. The cell-invasion ability was determined by a Matrigel invasion assay. Values are presented as the mean ± SE of three independent experiments. Data were analyzed using a one-way ANOVA with Tukey's post-hoc tests at 95% confidence intervals; different letters represent different levels of significance. E. DU145 cells were transfected with either an miR-23a-3p inhibitor or a negative control. The cell-invasion ability was determined by a Matrigel invasion assay. Values are presented as the mean ± SE of three independent experiments. * p < 0.05, compared to the control groups. F. PC3 or DU145 cells were treated with TGF-β for 6, 12, or 24 h. Expression levels of E-cadherin and miR-23a-3p were determined by Western blotting (upper panel) and a quantitative PCR (lower panel), respectively. Quantitative E-cadherin protein levels were adjusted to the β-actin protein level.
    Human Mirna Onearray ® Mirna Profiling Chip, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+and+high-throughput+sequencing+database/human+mirna+onearray+v4/pmc04673254-105-14-31
    Average 90 stars, based on 1 article reviews
    human mirna onearray ® mirna profiling chip - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "By inhibiting snail signaling and miR-23a-3p, osthole suppresses the EMT-mediated metastatic ability in prostate cancer"

    Article Title: By inhibiting snail signaling and miR-23a-3p, osthole suppresses the EMT-mediated metastatic ability in prostate cancer

    Journal: Oncotarget

    doi:

    A. A schematic representation of the procedure for miRNA selection. Differential expressions of miRNAs in osthole-treated cells versus vehicle-treated cells were analyzed with a OneArray ® miRNA profiling chip. B. Treatment of Du145 cells with osthole for 6 h. miR-146a, miR-22-3p, and miR-23a-3p expressions were detected by a quantitative PCR. C. Upper panel, Du145 cells were transfected with an miR-23a-3p mimic or mimic control for 24 h followed by osthole (60 μM) treatment for an additional 24 h. E-cadherin expression levels were determined by a Western blot analysis. Quantitative E-cadherin protein levels were adjusted to the β-actin protein level. Lower panel, Relative luciferase activities of DU145 cells co-transfected with an E-cadherin luciferase 3′UTR reporter vector and miR-23a-3p mimic or mimic control for 24 h. Values are presented as the mean ± SE of three independent experiments. * p < 0.05, compared to the control groups. D. DU145 cells were transfected with an miR-23a-3p mimic or mimic control for 24 h followed by osthole (60 μM) treatment for an additional 24 h. The cell-invasion ability was determined by a Matrigel invasion assay. Values are presented as the mean ± SE of three independent experiments. Data were analyzed using a one-way ANOVA with Tukey's post-hoc tests at 95% confidence intervals; different letters represent different levels of significance. E. DU145 cells were transfected with either an miR-23a-3p inhibitor or a negative control. The cell-invasion ability was determined by a Matrigel invasion assay. Values are presented as the mean ± SE of three independent experiments. * p < 0.05, compared to the control groups. F. PC3 or DU145 cells were treated with TGF-β for 6, 12, or 24 h. Expression levels of E-cadherin and miR-23a-3p were determined by Western blotting (upper panel) and a quantitative PCR (lower panel), respectively. Quantitative E-cadherin protein levels were adjusted to the β-actin protein level.
    Figure Legend Snippet: A. A schematic representation of the procedure for miRNA selection. Differential expressions of miRNAs in osthole-treated cells versus vehicle-treated cells were analyzed with a OneArray ® miRNA profiling chip. B. Treatment of Du145 cells with osthole for 6 h. miR-146a, miR-22-3p, and miR-23a-3p expressions were detected by a quantitative PCR. C. Upper panel, Du145 cells were transfected with an miR-23a-3p mimic or mimic control for 24 h followed by osthole (60 μM) treatment for an additional 24 h. E-cadherin expression levels were determined by a Western blot analysis. Quantitative E-cadherin protein levels were adjusted to the β-actin protein level. Lower panel, Relative luciferase activities of DU145 cells co-transfected with an E-cadherin luciferase 3′UTR reporter vector and miR-23a-3p mimic or mimic control for 24 h. Values are presented as the mean ± SE of three independent experiments. * p < 0.05, compared to the control groups. D. DU145 cells were transfected with an miR-23a-3p mimic or mimic control for 24 h followed by osthole (60 μM) treatment for an additional 24 h. The cell-invasion ability was determined by a Matrigel invasion assay. Values are presented as the mean ± SE of three independent experiments. Data were analyzed using a one-way ANOVA with Tukey's post-hoc tests at 95% confidence intervals; different letters represent different levels of significance. E. DU145 cells were transfected with either an miR-23a-3p inhibitor or a negative control. The cell-invasion ability was determined by a Matrigel invasion assay. Values are presented as the mean ± SE of three independent experiments. * p < 0.05, compared to the control groups. F. PC3 or DU145 cells were treated with TGF-β for 6, 12, or 24 h. Expression levels of E-cadherin and miR-23a-3p were determined by Western blotting (upper panel) and a quantitative PCR (lower panel), respectively. Quantitative E-cadherin protein levels were adjusted to the β-actin protein level.

    Techniques Used: Selection, Real-time Polymerase Chain Reaction, Transfection, Expressing, Western Blot, Luciferase, Plasmid Preparation, Invasion Assay, Negative Control

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    Article Title: Myeloid-derived suppressor cells shift Th17/Treg ratio and promote systemic lupus erythematosus progression through arginase-1/miR-322-5p/TGF-β pathway
    Article Snippet: The human miRNA OneArray v6 chip (Phalanx Biotech Group, Hsinchu, Taiwan) was performed to evaluate the co-differentially expressed miRNAs of the three groups (Supplementary Tables S1 and 2).

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    Article Snippet: We performed miRNA microarray validation (The Mouse & Rat miRNA OneArray ® , Phalanx Biotech Group, Hsinchu, Taiwan) and qRT-PCR analysis to compare the expression of miRNAs in the HFD-fed db/db mice and standard diet–fed db/db mice [ ].

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    Microarray:

    Article Title: Polyamines from myeloid-derived suppressor cells promote Th17 polarization and disease progression.
    Article Snippet: .. Microarray was performed using Human miRNA OneArray v6 chip (Phalanx Biotech Group) as recommended by the manufacturer. hsa-miR-542–5p transfection A total of 2 to 4 105 purified naive CD4+ T cells in 100 mL of 1640 medium were seeded per well into 96-well plates. .. HiPerFect transfection reagent (0.6 mL, Qiagen) and hsa-miR542–5p mimics, inhibitor, or control (0.3 mL, GenePharma) were prepared in 20 mL of complete 1640 medium.

    Article Title: The RNA-binding protein KSRP aggravates malignant progression of clear cell renal cell carcinoma through transcriptional inhibition and post-transcriptional destabilization of the NEDD4L ubiquitin ligase
    Article Snippet: Gene expression levels were normalized as log2 values using GeneSpring software (Agilent Technologies, Santa Clara, CA, USA), and the obtained list contained genes that were up- or downregulated with greater than twofold changes in response to KSRP-KD. .. To identify which miRNAs are regulated by KSRP, a high-throughput and specific miRNA microarray (human miRNA OneArray ® miRNA profiling chip) using Caki-1 cells with/without KSRP-KD was conducted by the Phalanx Biotech Group (Hsinchu, Taiwan). ..

    Transfection:

    Article Title: Polyamines from myeloid-derived suppressor cells promote Th17 polarization and disease progression.
    Article Snippet: .. Microarray was performed using Human miRNA OneArray v6 chip (Phalanx Biotech Group) as recommended by the manufacturer. hsa-miR-542–5p transfection A total of 2 to 4 105 purified naive CD4+ T cells in 100 mL of 1640 medium were seeded per well into 96-well plates. .. HiPerFect transfection reagent (0.6 mL, Qiagen) and hsa-miR542–5p mimics, inhibitor, or control (0.3 mL, GenePharma) were prepared in 20 mL of complete 1640 medium.

    Purification:

    Article Title: Polyamines from myeloid-derived suppressor cells promote Th17 polarization and disease progression.
    Article Snippet: .. Microarray was performed using Human miRNA OneArray v6 chip (Phalanx Biotech Group) as recommended by the manufacturer. hsa-miR-542–5p transfection A total of 2 to 4 105 purified naive CD4+ T cells in 100 mL of 1640 medium were seeded per well into 96-well plates. .. HiPerFect transfection reagent (0.6 mL, Qiagen) and hsa-miR542–5p mimics, inhibitor, or control (0.3 mL, GenePharma) were prepared in 20 mL of complete 1640 medium.



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