Microarray:Article Title: Evaluating the risk of patent infringement by means of semantic patent analysis: the case of DNA chips
Article Snippet: In this paper, we are going to present a method for detecting the risks of patent infringement by evaluating similarities between patent documents on the basis of semantic patent analysis.. This approach enables the user to visualize similarities in the contents on a semantic patent map by means of multi-dimensional scaling.. The effectiveness of the semantic patent map has already been demonstrated by Dressler (2006) with regard to patents of seal technology, in which documents are commonly kept short and the extracted contents are concise.
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Identifying Genes Regulated in a Myc-dependent Manner
Article Snippet: .. Identifying Differentially Expressed Genes Using a cDNA Microarray Approach—Fluorescently labeled cDNA representing mRNA from asynchronously growing HO15.19-GFP and HO15.19-myc cells was used to probe 6.3K cDNA microarrays in duplicate (Synteni). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Microarray technology GEM microarrays and drug discovery.
Article Snippet: Incyte Genomics’ GEM Gene Expression Microarray is a proven genomics tool used by a large number of pharmaceutical companies to speed up the drug discovery and development process.. The development and integration of this technology, together with Incyte’s sequence databases and clone resources, have resulted in GEM microarrays that span approximately 60,000 human genes as well as approximately 60,000 plant, rat, mouse, yeast, and bacterial genes.. The technology underlying the use of these arrays and their application to the drug discovery process is highlighted.
Produced:
Plasmid Preparation:Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Amplification:Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Polymerase Chain Reaction:Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Hybridization:Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Isolation:Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Labeling:Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Identifying Genes Regulated in a Myc-dependent Manner
Article Snippet: .. Identifying Differentially Expressed Genes Using a cDNA Microarray Approach—Fluorescently labeled cDNA representing mRNA from asynchronously growing HO15.19-GFP and HO15.19-myc cells was used to probe 6.3K cDNA microarrays in duplicate (Synteni). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Reverse Transcription:Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Derivative Assay:Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Cell Culture:Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
Article Title: Sequences characteristic of hypoxia-regulated gene transcription
Article Snippet: .. For this, the inserts of each plasmid were amplified by PCR and robotically fabricated on the glass. cDNA chip printing was performed by Synteni (Wang et al, 1999) Preparation of Probes for Microarray Hybridization Isolated messenger RNA is labeled with fluorescent dNTP's using a reverse transcription reaction, using 50 μg template RNA (probes derived from nuclear RNA and total RNA), to generate a labeled cDNA probe. mRNA is extracted from either C6 or A172 cells cultured in normoxia conditions and labeled with Cy3-dCTP (Amersham) and mRNA extracted from C6 or A172 cells cultured under hypoxic conditions is labeled with Cy5-dCTP (Amersham). ..
other:Article Title: DNA microarrays in medicine: manufacturing techniques and potential applications
Article Snippet: Since their first description in 1995, DNA microarrays have moved to centre stage in many areas of biomedical research and are now assuming an increasingly important role in diagnostics and in pharmacology.. Such microarrays may be used to detect and quantify nucleic acid and to determine its sequence.. In medicine, a major area of use is in detection of changes in the pattern of gene expression in conditions such as cancer.
|
