antifade mounting medium with dapi (Beyotime)
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99
Structured Review
Beyotime
antifade mounting medium with dapi
Antifade Mounting Medium With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/micro+manager+open+source+microscope+controller+software/DAPI/pmc13040898-195-3-8
Average 99 stars, based on 32901 article reviews
Antifade Mounting Medium With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/micro+manager+open+source+microscope+controller+software/DAPI/pmc13040898-195-3-8
Average 99 stars, based on 32901 article reviews
antifade mounting medium with dapi - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "A novel molecule ZYZ329 targeting histone methyltransferase SMYD3 suppresses pathological angiogenesis via the driven mitochondrial ROS/HIF-1α/VEGFA axis"
Article Title: A novel molecule ZYZ329 targeting histone methyltransferase SMYD3 suppresses pathological angiogenesis via the driven mitochondrial ROS/HIF-1α/VEGFA axis
Journal: Journal of Translational Medicine
doi: 10.1186/s12967-025-07580-2
Figure Legend Snippet: SMYD3 inhibition attenuated angiogenesis in vitro and in vivo. ( A ) HUVEC were transduced with lentiviral ShRNA (ShRNA1 and ShRNA2) or control ShRNA (Sh-Ctrl) for 48 hours. SMYD3 and H3K4me3 protein expression was quantified by Western blot, with GAPDH and total histone H3 serving as internal reference ( n = 3). ( B ) The VEGF-containing Matrigel plugs with Sh-SMYD3 or Sh-Ctrl were harvested from WT mice after 14 days ( n = 8). Bar = 3 mm. ( C, D ) Sections of Matrigel plugs were stained with H&E or CD31 (green) and DAPI (blue). Representative images and quantification of CD31 positive areas were shown ( n = 8). Bar = 100 μm. ( E ) HUVEC were incubated with Sh-SMYD3 and then seeded on Matrigel; tube formation was captured (upper panel) and relative tube length were analyzed (lower panel). n = 6. Bar = 100 μm. ( F ) the thoracic aortic ring isolated from WT mice were cultured in VEGF-containing Matrigel with Sh-SMYD3 or Sh-Ctrl. Sprouting was captured (lower panel) and sprouting/explant ratio was calculated (upper panel). n = 8. Bar = 100 μm. ( G ) The CAM was photographed after incubating with Sh-SMYD3 or Sh-Ctrl ( n = 6). Wound healing assay ( H ), cell counts ( I ), and nitric oxide ( J ) were measured ( n = 6). Bar = 100 μm. Unpaired Student’s t test ( D, E, F, H, J ), One-way ANOVA ( A ) and Two-way ANOVA ( I ) coupled with Bonferroni post hoc test were used. ns p > 0.05, ** p < 0.01, *** p < 0.001
Techniques Used: Inhibition, In Vitro, In Vivo, Transduction, shRNA, Control, Expressing, Western Blot, Staining, Incubation, Isolation, Cell Culture, Wound Healing Assay
Figure Legend Snippet: Overexpression of SMYD3 promoted angiogenesis. ( A ) HUVEC were transfected with Sh-Ctrl, Sh-SMYD3, and Sh-SMYD3 plus SMYD3-Res lentiviruses for 48 hours. SMYD3 and β-tubulin protein expression was quantified by Western blot ( n = 3). ( B ) The VEGF-containing Matrigel plugs with Sh-Ctrl, Sh-SMYD3, and Sh-SMYD3 plus SMYD3-Res lentiviruses were harvested from WT mice after 14 days ( n = 6). Bar = 4 mm. ( C ) Sections of Matrigel plugs were stained with CD31 (green) and DAPI (blue). Representative images and quantification of CD31 positive areas were shown ( n = 6). Bar = 100 μm. ( D ) HUVEC were incubated with Sh-Ctrl, Sh-SMYD3, and Sh-SMYD3 plus SMYD3-Res and then seeded on Matrigel; tube formation was captured and relative tube length was analyzed ( n = 5). Bar = 100 μm. ( E ) Matrigel plugs and ( F ) CD31 immunofluorescence staining ( n = 6). Bar = 100 μm. ( G ) Aortic ring sprouting ( n = 5). Bar = 100 μm. ( H ) Tube formation stained with Calcein AM (green) ( n = 5). Bar = 100 μm. Data shown are for Ad-SMYD3 and Ad-Ctrl groups. Unpaired Student’s t test ( F, G, H ), One-way ANOVA ( A, C, D ) coupled with Bonferroni post hoc test were used. ** p < 0.01, *** p < 0.001
Techniques Used: Over Expression, Transfection, Expressing, Western Blot, Staining, Incubation, Immunofluorescence
Figure Legend Snippet: ZYZ329 significantly attenuated the angiogenic ability of endothelial cells. Matrigel plugs containing 10 μM ZYZ329, EPZ031686, or DMSO were sectioned and stained with H&E for histological analysis ( A ) and CD31 (green)/DAPI (blue) for immunofluorescence detection ( B ). Bar = 100 μm. Representative images were shown, and the CD31-positive area was quantified ( D , n = 6). Aortic ring sprouting area was photographed ( C ) and quantified as sprouting/explant ratio ( E , n = 6). Bar = 100 μm. Endothelial tube formation in Matrigel was captured ( G ), and the corresponding relative tube length was quantified ( F , n = 6). Bar = 100 μm. ( H ) The wound closure ratio was analyzed ( n = 6). ( I ) HUVEC proliferation was assessed by cell counting on day 0 and day 3 post-treatment with 10 μM ZYZ329, EPZ031686, or DMSO ( n = 6). ( J ) Measurement of NO production in HUVEC stimulated by VEGF after treatment with 10 μM ZYZ329, EPZ031686, or DMSO ( n = 6). Matrigel plugs ( K ) and corresponding CD31 staining ( L ) following treatment with 1 µM, 5 µM, and 10 µM ZYZ329 ( n = 6). ( K ) Bar = 4 mm. ( L ) Bar = 100 μm. ( M ) Aortic ring assay under treatment with 1 µM, 5 µM, and 10 µM ZYZ329 ( n = 5). Bar = 100 μm. One-way ANOVA ( D, E, F, H, J, L, M ) and Two-way ANOVA ( I ) coupled with Bonferroni post hoc test were used. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
Techniques Used: Staining, Immunofluorescence, Cell Counting, Aortic Ring Assay
Figure Legend Snippet: ZYZ329 inhibited pathological angiogenesis in vivo. ( A ) Establishment and monitoring of murine hindlimb ischemia model. Schematic in this panel was created using BioRender (https://www.biorender.com/). ( B, C ) Vehicle, ZYZ329, and EPZ031686 were administered via intraperitoneal injection (25 mg/kg) for 14 consecutive days. The Sham group underwent skin incision without femoral artery ligation. Hindlimb perfusion was quantitatively assessed using laser speckle imaging, with ischemic/non-ischemic hindlimb perfusion ratio calculated pre-ligation and at postoperative days 0, 3, 7, and 14 ( n = 8). ( D ) High resolution laser speckle imaging was employed to characterize vascular network architecture in ischemic hindlimbs across experimental groups: Sham, Vehicle, ZYZ329, and EPZ031686 ( n = 6). ( E ) On postoperative day 14, micro-CT angiography was performed following injection of angiographic contrast agent, enabling three-dimensional reconstruction and analysis of collateral vessel distribution in ischemic hindlimbs ( n = 6). ( F ) H&E staining of ischemic hindlimb muscles in the Sham, Vehicle, ZYZ329, and EPZ031686 groups. Myofibers with central nuclei in the injured/regenerating areas are indicated by arrows ( n = 8). Bar = 50 μm. ( G ) Representative images of immunofluorescence staining of CD31 (green) and DAPI (blue) in ischemic hindlimb muscles in the Sham, Vehicle, ZYZ329, and EPZ031686 groups ( n = 8). Bar = 50 μm. ( H ) Percentage quantification of the injured/regenerating areas in ischemic hindlimb sections ( n = 8). ( I ) Analysis of CD31 staining fluorescence intensity in Sham, Vehicle, ZYZ329, and EPZ031686 groups ( n = 8). Two-way ANOVA ( C ) and One-way ANOVA ( H, I ) coupled with Bonferroni post hoc test were used. ** p < 0.01, *** p < 0.001
Techniques Used: In Vivo, Injection, Ligation, Imaging, Micro-CT, Staining, Muscles, Immunofluorescence, Fluorescence
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