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polyclonal goat anti megalin antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology polyclonal goat anti megalin antibody

    Polyclonal Goat Anti Megalin Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/megalign+software/megalin+Antibody/pmc07856474-8-0-5
    Average 93 stars, based on 142 article reviews
    polyclonal goat anti megalin antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation"

    Article Title: In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2021.100314


    Figure Legend Snippet:

    Techniques Used: Recombinant, Blocking Assay, Gene Expression, TaqMan Assay, Software, Fluorescence, Microscopy

    Related Articles

    other:

    Article Title: NNMT inhibition counteracts tubular senescence and fibrosis in early stages of chronic kidney disease.
    Article Snippet: Slides were mounted using VectaMount mounting medium (Vectorlabs) and scanned with Philips IntelliSite Ultra Fast Scanner.

    Incubation:

    Article Title: Heterogeneity in lysosomal dynamics and metabolic functions along the kidney proximal tubule.
    Article Snippet: .. Stainings were performed on 5 μm thick cryosections incubated overnight with the following antibodies: mouse anti-Megalin (H10) (1:100, Santa cruz Biotech, sc-515772), rabbit anti-Rab5 (1:50, Cell signaling, #3547), AR TI CL E IN P RE SS rabbit anti-Rab7 (1:50, Abcam, ab137029), goat anti-Cathepsin L (1:100, R&D Systems, AF1515), rabbit anti-OAT1 (1:100, Alpha diagnostic international, OAT11-A). .. LAL staining was performed in paraffin embedded tissues, following a citrate pH 6 buffer antigen retrieval step at high temperature and pressure, using mouse anti-LAL (1:100, Santa cruz Biotech, sc-58374).

    Article Title: Lipocalin‐2 Restores Soluble Guanylyl Cyclase‐Dependent Dilation of the Afferent Arteriole After Renal Transplantation or Ex Vivo Hypoxia/Reoxygenation in Mice
    Article Snippet: The membranes were blocked for 1 h at room temperature with 5% skim milk (Applichem, Darmstadt, Germany) and 1% bovine serum albumin (SERVA, Heidelberg, Germany), dissolved in TBST (50 mM TRIZMA base, 150 mM NaCl, pH 7.5 adjusted with HCl, 0.1% Tween‐20). .. The membranes were then incubated overnight at 4°C with the following primary antibodies: anti‐Lrp2 (sc‐515772, Santa Cruz Biotechnology, CA, USA), anti‐Akt (#9272, Cell Signaling Technologies). ..

    Article Title: Single‐Chain Anti‐IL‐1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4°C: anti‐His (MA5‐33032, Invitrogen; 1:2000 dilution) and anti‐megalin (sc‐515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Article Title: Lipocalin-2 Restores Soluble Guanylyl Cyclase-Dependent Dilation of the Afferent Arteriole After Renal Transplantation or Ex Vivo Hypoxia/Reoxygenation in Mice.
    Article Snippet: The membranes were blocked for 1 h at room temperature with 5% skim milk (Applichem, Darmstadt, Germany) and 1% bovine serum albumin (SERVA, Heidelberg, Germany), dissolved in TBST (50 mM TRIZMA base, 150 mM NaCl, pH 7.5 adjusted with HCl, 0.1% Tween- 20). .. The membranes were then incubated overnight at 4°C with the following primary antibodies: anti- Lrp2 (sc- 515772, Santa Cruz Biotechnology, CA, USA), antiAkt (#9272, Cell Signaling Technologies). ..

    Article Title: Single-Chain Anti-IL-1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease.
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4◦C: anti-His (MA5-33032, Invitrogen; 1:2000 dilution) and anti-megalin (sc-515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Biomarker Discovery:

    Article Title:
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used anti-Wilms’ tumor-1 (WT1; ab89901, Abcam plc. .. / sc-7385, Santa Cruz Biotechnology Inc.); anti-cytokeratin 8 (CK8 (TROMA-I); DSHB); anti-human nuclei (HuNu; MAB4383, Merck Millipore); anti-Ku80 (2180S, Cell Signaling Technology, Inc.); anti-lotus tetragonolobus 3 nature portfolio | reporting sum m ary M arch 2021 lectin (LTL; B-1325, Vector Laboratories, Inc.); anti-E-cadherin-1 (ECAD; 610181, BD Biosciences); anti-nephrin (GP-N2, Vector Laboratories, Inc.); anti-podocalyxin (PODXL; AF1658, R&D Systems, Inc.); anti-Jagged 1 (JAG1; sc-390177, Santa Cruz Biotechnology Inc.); anti-cadherin 6 (CDH6; AF1658, R&D Systems, Inc.); anti-aquaporin 1 (AQP1; ab9566, Abcam plc.); anti-megalin (sc-515772, Santa Cruz Biotechnology Inc.); anti-Dolichos biflorus agglutinin (DBA; B-1035, Vector Laboratories, Inc.); anti-sodium-chloride cotransporter (NCC; AB3553, Merck Millipore); anti-integrin alpha 8(ITGA8; BAF4076, R&D Systems, Inc.); anti-platlet-derived growth factor receptor alpha (PDGFRA; 323506, BioLegend, Inc.) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer. .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used anti-Wilms’ tumor-1 (WT1; ab89901, Abcam plc.

    Blocking Assay:

    Article Title: Single‐Chain Anti‐IL‐1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4°C: anti‐His (MA5‐33032, Invitrogen; 1:2000 dilution) and anti‐megalin (sc‐515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Article Title: Single-Chain Anti-IL-1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease.
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4◦C: anti-His (MA5-33032, Invitrogen; 1:2000 dilution) and anti-megalin (sc-515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Staining:

    Article Title: Single‐Chain Anti‐IL‐1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4°C: anti‐His (MA5‐33032, Invitrogen; 1:2000 dilution) and anti‐megalin (sc‐515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Article Title: Single-Chain Anti-IL-1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease.
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4◦C: anti-His (MA5-33032, Invitrogen; 1:2000 dilution) and anti-megalin (sc-515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.



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