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clustal x  (DNASTAR)


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    Structured Review

    DNASTAR clustal x
    Clustal X, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 11736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mega+6+program/Lasergene/pm35569913-95-5-8
    Average 99 stars, based on 11736 article reviews
    clustal x - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Software:

    Article Title: First Isolation, Genomic Characterization and Experimental Infection of Bovine Coronavirus from Sheep
    Article Snippet: PCR products were examined by agarose gel electrophoresis, purified using a PCR purification kit (Tiangen Biotech Co., Ltd., Beijing, China; Cat. No. DP214-02), cloned into the pMD19-T vector (Takara, Japan; Cat. No. 6013), and subjected to Sanger sequencing by Chengdu Qinke Biotechnology Co., Ltd. (Chengdu, China). .. The Sangerderived sequences were aligned with the metagenomic assemblies using SeqMan software in the DNASTAR Lasergene package version 7.1 (DNASTAR Inc., Madison, WI, USA). ..

    Article Title: Restoration of infectivity-virulence and toxin biosynthesis in Botrytis cinerea B459 via upstream-targeted marker-free CRISPR/Cas9 editing of the transcription factor Bcin04g03490.
    Article Snippet: For sgRNA purification, RNA Clean and Concentrator 25 (ref.Rl O18; Zymo Research; USA) was used. .. Sequence analysis utilized software from the DNASTAR Lasergene package (DNASTAR; USA). .. Oligonucleotides (Table S1) were sourced from Metabion (Metabion International AG; Germany).

    Article Title: Co-circulation of H5N1 and H5N6 high pathogenicity avian influenza viruses in migratory birds at a coastal wintering site in China.
    Article Snippet: The amplified products were sent to Comate Bioscience Co., Ltd. (Changchun, China) for Sanger sequencing. .. Viral gene segments were analysed using the DNAStar Lasergene software package. ..

    Article Title: Immunogenicity of recombinant porcine parainfluenza virus 5 expressing the spike protein of porcine epidemic diarrhea virus.
    Article Snippet: The porcine epidemic diarrhea virus (PEDV) induces severe diarrhea in neonatal piglets, often resulting in high mortality rates.. Consequently, the development of effective and safe vaccines is a critical priority for controlling PEDV infection.. Vaccines vectored by the parainfluenza virus 5 (PIV5) exhibit distinct advantages in terms of safety, genomic stability, and immunogenicity.

    Article Title: Otx2 directly regulates tbx3 expression during early eye formation.
    Article Snippet: After identification of 10k base pairs upstream and downstream the tbx3 gene for each species in NCBI:Gene or Ensembl.org, ECR were identified using ECR Browser (http://ecrbrowser.dcode.org) and MultiPIPMaker, as previously described (Ogino et al., 2006; Ledford et al., 2017). .. All sequence analysis was performed using the Lasergene Software Package (DNASTAR, Madison, WI). .. Putative transcription factor binding sites were identified using rVista (rvista.dcode.org) with a TRANSFAC professional v10.2 library.

    Article Title: Structural analyses of a heterodimeric SusCD complex captures intermediate states of maltooligosaccharide transport.
    Article Snippet: .. Burland, T. G. (2000) DNASTAR's Lasergene sequence analysis software. ..

    Article Title: Chitinase production and effects of land use change on fungal communities in the soil of the Brazilian Cerrado.
    Article Snippet: The resulting amplicons were subjected to Sanger sequencing at Instituto René Rachou (FIOCRUZ Minas). .. Sanger sequencing chromatograms were processed using DNASTAR Lasergene software to generate consensus ITS sequences. ..

    Sequencing:

    Article Title: Restoration of infectivity-virulence and toxin biosynthesis in Botrytis cinerea B459 via upstream-targeted marker-free CRISPR/Cas9 editing of the transcription factor Bcin04g03490.
    Article Snippet: For sgRNA purification, RNA Clean and Concentrator 25 (ref.Rl O18; Zymo Research; USA) was used. .. Sequence analysis utilized software from the DNASTAR Lasergene package (DNASTAR; USA). .. Oligonucleotides (Table S1) were sourced from Metabion (Metabion International AG; Germany).

    Article Title: Immunogenicity of recombinant porcine parainfluenza virus 5 expressing the spike protein of porcine epidemic diarrhea virus.
    Article Snippet: The porcine epidemic diarrhea virus (PEDV) induces severe diarrhea in neonatal piglets, often resulting in high mortality rates.. Consequently, the development of effective and safe vaccines is a critical priority for controlling PEDV infection.. Vaccines vectored by the parainfluenza virus 5 (PIV5) exhibit distinct advantages in terms of safety, genomic stability, and immunogenicity.

    Article Title: Otx2 directly regulates tbx3 expression during early eye formation.
    Article Snippet: After identification of 10k base pairs upstream and downstream the tbx3 gene for each species in NCBI:Gene or Ensembl.org, ECR were identified using ECR Browser (http://ecrbrowser.dcode.org) and MultiPIPMaker, as previously described (Ogino et al., 2006; Ledford et al., 2017). .. All sequence analysis was performed using the Lasergene Software Package (DNASTAR, Madison, WI). .. Putative transcription factor binding sites were identified using rVista (rvista.dcode.org) with a TRANSFAC professional v10.2 library.

    Article Title: Structural analyses of a heterodimeric SusCD complex captures intermediate states of maltooligosaccharide transport.
    Article Snippet: .. Burland, T. G. (2000) DNASTAR's Lasergene sequence analysis software. ..

    Analysis:

    Article Title: Restoration of infectivity-virulence and toxin biosynthesis in Botrytis cinerea B459 via upstream-targeted marker-free CRISPR/Cas9 editing of the transcription factor Bcin04g03490.
    Article Snippet: For sgRNA purification, RNA Clean and Concentrator 25 (ref.Rl O18; Zymo Research; USA) was used. .. Sequence analysis utilized software from the DNASTAR Lasergene package (DNASTAR; USA). .. Oligonucleotides (Table S1) were sourced from Metabion (Metabion International AG; Germany).

    Article Title: Otx2 directly regulates tbx3 expression during early eye formation.
    Article Snippet: After identification of 10k base pairs upstream and downstream the tbx3 gene for each species in NCBI:Gene or Ensembl.org, ECR were identified using ECR Browser (http://ecrbrowser.dcode.org) and MultiPIPMaker, as previously described (Ogino et al., 2006; Ledford et al., 2017). .. All sequence analysis was performed using the Lasergene Software Package (DNASTAR, Madison, WI). .. Putative transcription factor binding sites were identified using rVista (rvista.dcode.org) with a TRANSFAC professional v10.2 library.

    Article Title: Structural analyses of a heterodimeric SusCD complex captures intermediate states of maltooligosaccharide transport.
    Article Snippet: .. Burland, T. G. (2000) DNASTAR's Lasergene sequence analysis software. ..

    Hemagglutination Assay:

    Article Title: H9N2 avian influenza virus in Muscovy ducks: a nationwide epidemiological survey and vaccine efficacy assessment.
    Article Snippet: For molecular identification, reverse transcription (RT)-PCR was performed to amplify the HA genes via specific primers (HA-F: 5′- CAA GAT GGA AGT AGT ATC ACT-3′; HA-R: 5′- TTG CCA ATT ATA TAC AAA TGT − 3′), and the resulting PCR products were sequenced by Shanghai Sangon Biotech. .. Nucleotide sequences of the HA gene were assembled via DNAstar Lasergene 11. ..

    DNA Sequencing:

    Article Title: Chitinase production and effects of land use change on fungal communities in the soil of the Brazilian Cerrado.
    Article Snippet: The resulting amplicons were subjected to Sanger sequencing at Instituto René Rachou (FIOCRUZ Minas). .. Sanger sequencing chromatograms were processed using DNASTAR Lasergene software to generate consensus ITS sequences. ..



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    Image Search Results


    Phylogenetic analysis of the porcine epidemic diarrhea virus isolates based on the nucleotide sequences of the partials S genes. The tree was generated by the maximum likelihood method of the software MEGA v.6.05. Numbers at nodes represent the percentage of 1000 bootstrap replicates (values <70 are not shown). The scale bar indicates nucleotide substitution per site. The recent Japanese PEDV isolates in this study are marked by solid round symbols, the Japanese strains prior to 2013 are marked by solid diamond symbols , and the vaccine strains being used in Japan are marked by solid square symbols . The US strains and US-like strains are marked by triangle hollow symbols , while the S INDEL strains are marked by solid triangle symbols

    Journal: SpringerPlus

    Article Title: US-like isolates of porcine epidemic diarrhea virus from Japanese outbreaks between 2013 and 2014

    doi: 10.1186/s40064-015-1552-z

    Figure Lengend Snippet: Phylogenetic analysis of the porcine epidemic diarrhea virus isolates based on the nucleotide sequences of the partials S genes. The tree was generated by the maximum likelihood method of the software MEGA v.6.05. Numbers at nodes represent the percentage of 1000 bootstrap replicates (values <70 are not shown). The scale bar indicates nucleotide substitution per site. The recent Japanese PEDV isolates in this study are marked by solid round symbols, the Japanese strains prior to 2013 are marked by solid diamond symbols , and the vaccine strains being used in Japan are marked by solid square symbols . The US strains and US-like strains are marked by triangle hollow symbols , while the S INDEL strains are marked by solid triangle symbols

    Article Snippet: Phylogenetic trees based on the nucleotide sequences of the partial S gene and ORF3 gene were constructed with maximum likelihood method using Hasegawa-Kishino-Yano substitution model with discrete Gamma distribution, and bootstrap tests of 1000 replicates in the MEGA v.6 program.

    Techniques: Virus, Generated, Software

    Phylogenetic analysis of the porcine epidemic diarrhea virus isolates based on the ORF3 of PEDV. The tree was generated by the maximum likelihood method of the software MEGA v.6.05. Numbers at nodes represent the percentage of 1000 bootstrap replicates (values <70 are not show). The scale bar indicates nucleotide substitution per site. The recent Japanese PEDV isolates in this study are marked by solid round symbols and the vaccine strains being used in Japan are marked by solid square symbols . The US strains and US-like strains are marked by triangle hollow symbols , while the S INDEL strains are marked by solid triangle symbols

    Journal: SpringerPlus

    Article Title: US-like isolates of porcine epidemic diarrhea virus from Japanese outbreaks between 2013 and 2014

    doi: 10.1186/s40064-015-1552-z

    Figure Lengend Snippet: Phylogenetic analysis of the porcine epidemic diarrhea virus isolates based on the ORF3 of PEDV. The tree was generated by the maximum likelihood method of the software MEGA v.6.05. Numbers at nodes represent the percentage of 1000 bootstrap replicates (values <70 are not show). The scale bar indicates nucleotide substitution per site. The recent Japanese PEDV isolates in this study are marked by solid round symbols and the vaccine strains being used in Japan are marked by solid square symbols . The US strains and US-like strains are marked by triangle hollow symbols , while the S INDEL strains are marked by solid triangle symbols

    Article Snippet: Phylogenetic trees based on the nucleotide sequences of the partial S gene and ORF3 gene were constructed with maximum likelihood method using Hasegawa-Kishino-Yano substitution model with discrete Gamma distribution, and bootstrap tests of 1000 replicates in the MEGA v.6 program.

    Techniques: Virus, Generated, Software