Journal: bioRxiv
Article Title: Mechanisms of Pathogenicity of Hypertrophic Cardiomyopathy-Associated Troponin T (TNNT2) Variant R278C +/− During Development
doi: 10.1101/2023.06.06.542948
Figure Lengend Snippet: Panel A. GFP-tagged α-actinin hiPSC-CMs cultured on 5 kPa PDMS (left) and SarcTrack wavelet fitting (right). Panel B. The relaxation of WT (black, n=9) and R278C +/− (red, n=12) hiPSC-CMs cultured on 5 kPa PDMS, with respect to: B1) time-to-half maximal relaxation (ms). B2) the maximum velocity of relaxation (μm . s −1 ). Panel C. The contractile parameters of WT (black, n=9) and R278C +/− (red, n=12) hiPSC-CMs cultured on 5 kPa PDMS and stimulated at 1 Hz with respect to: C1) percent maximal shortening; C2) time to half maximal contraction; C3) maximum velocity of contraction (μm s −1 ). (* p-value< 0.05, ** p-value <0.005, ***p-value<0.0005)
Article Snippet: To examine the contractile properties of R278C, we employed the newly developed Matlab-based algorithm, SarcTrack, to quantify sarcomeric shortening in α-actinin 2-GFP tagged hiPSC-CMs.
Techniques: Cell Culture