methylflashtm methylated dna quantification kit (EpiGentek)
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Methylflashtm Methylated Dna Quantification Kit, supplied by EpiGentek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/massarray+quantitative+methylation+analysis/methylflash+methylated+dna+quantification+kit/pmc10160113-75-7-12
Average 90 stars, based on 1 article reviews
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1) Product Images from "MBD2 facilitates tumor metastasis by mitigating DDB2 expression"
Article Title: MBD2 facilitates tumor metastasis by mitigating DDB2 expression
Journal: Cell Death & Disease
doi: 10.1038/s41419-023-05804-1
Figure Legend Snippet: A The B16F10 melanoma tumor volume in each group was determined at the indicated time points. B Images of tumor nodules at the endpoint of the experiment. C The weight of the tumor nodules from each group at the endpoint of the experiment. D Western blot analysis of MBD2 in each group. E , F RT-PCR analysis of Cdh1 ( E ) and Cdh2 ( F ) in the indicated groups. G Representative images of E-cadherin and N-cadherin coimmunostaining in the tumor nodule sections. H Images of pulmonary metastatic nodules from the indicated groups. I Representative H&E staining of pulmonary metastatic nodules from the indicated groups. J MBD2 binds to methylated CpG DNA within the DDB2 promoter and thus represses DDB2 expression, resulting in tumor metastasis. Intravenous administration of liposomes carrying MBD2 siRNA suppressed tumor growth in an animal model bearing tumor metastasis. L-Scr siRNA: liposome-based Scrambled siRNA; L- MBD2 siRNA: liposome-based MBD2 siRNA. The data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Western Blot, Reverse Transcription Polymerase Chain Reaction, Staining, Methylation, Expressing, Liposomes, Animal Model
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