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sequenom massarray methylation  (Sequenom)

 
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    Sequenom sequenom massarray methylation
    Fig. 4. Cd interfered with the DNA <t>methylation</t> of Oct4 and Nanog of mouse ES cells into ovarian GCs. A and E represented the methylation status of CpG islands on DNA templates in the promoter regions of both factors was evaluated using the Sequenom <t>MassARRAY</t> Methylation. B and F: DNA methylation rate of CpG locus in the promoter region of different sites of both factors. C–K represented the degree of DNA methylation on different fragments of the factors in maps and DNA methylation rate in the promoter region in each fragment and groups. Data are presented as the mean ± SD, *P < 0.05 compared with the IA group.
    Sequenom Massarray Methylation, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray+analysis/analysis+massarray+methylation+sequenom/pm34871855-191-17-17
    Average 86 stars, based on 1 article reviews
    sequenom massarray methylation - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Ultra-high field spinal cord MRI in multiple sclerosis: Where are we standing? A literature review."

    Article Title: Ultra-high field spinal cord MRI in multiple sclerosis: Where are we standing? A literature review.

    Journal: Multiple sclerosis and related disorders

    doi: 10.1016/j.msard.2021.103436

    Fig. 4. Cd interfered with the DNA methylation of Oct4 and Nanog of mouse ES cells into ovarian GCs. A and E represented the methylation status of CpG islands on DNA templates in the promoter regions of both factors was evaluated using the Sequenom MassARRAY Methylation. B and F: DNA methylation rate of CpG locus in the promoter region of different sites of both factors. C–K represented the degree of DNA methylation on different fragments of the factors in maps and DNA methylation rate in the promoter region in each fragment and groups. Data are presented as the mean ± SD, *P < 0.05 compared with the IA group.
    Figure Legend Snippet: Fig. 4. Cd interfered with the DNA methylation of Oct4 and Nanog of mouse ES cells into ovarian GCs. A and E represented the methylation status of CpG islands on DNA templates in the promoter regions of both factors was evaluated using the Sequenom MassARRAY Methylation. B and F: DNA methylation rate of CpG locus in the promoter region of different sites of both factors. C–K represented the degree of DNA methylation on different fragments of the factors in maps and DNA methylation rate in the promoter region in each fragment and groups. Data are presented as the mean ± SD, *P < 0.05 compared with the IA group.

    Techniques Used: DNA Methylation Assay, Methylation

    Fig. 5. Cd interfered with the DNA methylation of Amhr2 and Sox2 of mouse ES cells into ovarian GCs. A and E represented the methylation status of CpG islands on DNA templates in the promoter regions of both factors was evaluated using the Sequenom MassARRAY Methylation. B and F: DNA methylation rate of CpG locus in the promoter region of different sites of both factors. C-K represented the degree of DNA methylation on different fragments of the factors in maps and DNA methylation rate in the promoter region in each fragment and groups. Data are presented as the mean ± SD, *P < 0.05 compared with the IA group.
    Figure Legend Snippet: Fig. 5. Cd interfered with the DNA methylation of Amhr2 and Sox2 of mouse ES cells into ovarian GCs. A and E represented the methylation status of CpG islands on DNA templates in the promoter regions of both factors was evaluated using the Sequenom MassARRAY Methylation. B and F: DNA methylation rate of CpG locus in the promoter region of different sites of both factors. C-K represented the degree of DNA methylation on different fragments of the factors in maps and DNA methylation rate in the promoter region in each fragment and groups. Data are presented as the mean ± SD, *P < 0.05 compared with the IA group.

    Techniques Used: DNA Methylation Assay, Methylation

    Related Articles

    Methylation:

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics
    Article Snippet: .. Sequenom MassARRAY methylation analysis was used to detect the HIF-1α−25 promoter methylation level (Figure and Tables and ). ..

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics.
    Article Snippet: .. Sequenom MassARRAY methylation analysis was used to detect the HIF-1α−25 promoter methylation level (Figure S1 and Tables 3 and 4). ..

    Article Title: SNCA inhibits breast cancer progression through TP53AIP1–p53-mediated mitochondrial apoptosis and suppression of cancer stemness
    Article Snippet: .. 320 321 Sequenom mass ARRAY methylation analysis 322 Genomic DNA extracted from BC tissues was treated with sodium bisulfite using the 323 BisulFlash DNA Modification Kit (EpiGentek, Farmingdale, New York, USA) 324 following the manufacturer's guidelines. .. The methylation status of the SNCA 325 promoter region was evaluated using the Seuenom Mass ARRAY platform provided 326 by BioMiao Biological Technology.

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics.
    Article Snippet: .. Alcohol administration enhanced HIF-1α−25 promoter methylation in rats at weeks 8 and 10 Sequenom MassARRAY methylation analysis was conducted to detect the HIF-1α−25 promoter methylation status in the Control and Alcohol groups at weeks 6, 8, and 10 (Table 2 and Figure S1). ..

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics.
    Article Snippet: .. Methylation-Specific PCR, Sequenom MassARRAY methylation, ELISA, Western blot, and correlation analysis of 16S rRNA sequencing and metabolomics were performed. ..

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics.
    Article Snippet: .. Measurement of methylation level was conducted by quantitative methylation analysis (Sequenom). ..

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics
    Article Snippet: Note: AP: Acute Pancreatitis; AST: Aspartate Aminotransferase; ALT: Alanine Aminotransferase; GGT: Gamma-Glutamyl Transferase; BUN: Blood Urea Nitrogen; ALP: Alkaline Phosphatase; CREA: Creatinine; HE: Hematoxylin and Eosin; ELISA: Enzyme-Linked Immunosorbent Assay; HIF-1α: Hypoxia-inducible factor 1 alpha; VEGF: Vascular Endothelial Growth Factor .. Sequenom MassARRAY methylation analysis was conducted to detect the HIF-1α−25 promoter methylation status in the Control and Alcohol groups at weeks 6, 8, and 10 (Table and Figure ). ..

    Modification:

    Article Title: SNCA inhibits breast cancer progression through TP53AIP1–p53-mediated mitochondrial apoptosis and suppression of cancer stemness
    Article Snippet: .. 320 321 Sequenom mass ARRAY methylation analysis 322 Genomic DNA extracted from BC tissues was treated with sodium bisulfite using the 323 BisulFlash DNA Modification Kit (EpiGentek, Farmingdale, New York, USA) 324 following the manufacturer's guidelines. .. The methylation status of the SNCA 325 promoter region was evaluated using the Seuenom Mass ARRAY platform provided 326 by BioMiao Biological Technology.

    Control:

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics.
    Article Snippet: .. Alcohol administration enhanced HIF-1α−25 promoter methylation in rats at weeks 8 and 10 Sequenom MassARRAY methylation analysis was conducted to detect the HIF-1α−25 promoter methylation status in the Control and Alcohol groups at weeks 6, 8, and 10 (Table 2 and Figure S1). ..

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics
    Article Snippet: Note: AP: Acute Pancreatitis; AST: Aspartate Aminotransferase; ALT: Alanine Aminotransferase; GGT: Gamma-Glutamyl Transferase; BUN: Blood Urea Nitrogen; ALP: Alkaline Phosphatase; CREA: Creatinine; HE: Hematoxylin and Eosin; ELISA: Enzyme-Linked Immunosorbent Assay; HIF-1α: Hypoxia-inducible factor 1 alpha; VEGF: Vascular Endothelial Growth Factor .. Sequenom MassARRAY methylation analysis was conducted to detect the HIF-1α−25 promoter methylation status in the Control and Alcohol groups at weeks 6, 8, and 10 (Table and Figure ). ..

    Polymerase Chain Reaction:

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics.
    Article Snippet: .. Methylation-Specific PCR, Sequenom MassARRAY methylation, ELISA, Western blot, and correlation analysis of 16S rRNA sequencing and metabolomics were performed. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics.
    Article Snippet: .. Methylation-Specific PCR, Sequenom MassARRAY methylation, ELISA, Western blot, and correlation analysis of 16S rRNA sequencing and metabolomics were performed. ..

    Western Blot:

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics.
    Article Snippet: .. Methylation-Specific PCR, Sequenom MassARRAY methylation, ELISA, Western blot, and correlation analysis of 16S rRNA sequencing and metabolomics were performed. ..

    Sequencing:

    Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics.
    Article Snippet: .. Methylation-Specific PCR, Sequenom MassARRAY methylation, ELISA, Western blot, and correlation analysis of 16S rRNA sequencing and metabolomics were performed. ..



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    Image Search Results


    Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Journal: Discover Oncology

    Article Title: CTHRC1 modulates cell proliferation and invasion in hepatocellular carcinoma by DNA methylation

    doi: 10.1007/s12672-024-01194-8

    Figure Lengend Snippet: Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Article Snippet: Agena MassARRAY® Methylation Analysis assessed the methylation level of CTHRC1 in the promoter region.

    Techniques: Expressing, DNA Methylation Assay, Methylation, Quantitative RT-PCR, Western Blot