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Arraystar inc super rna labeling kits
Super Rna Labeling Kits, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lncrna+microarray+scanner/super+rna+labeling+kit/pm39869159-176-17-20
Average 90 stars, based on 1 article reviews
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Modification:

Article Title: Exploring the Roles of m6A-Modified circRNAs in Myasthenia Gravis Based on Multi-Omics Analysis.
Article Snippet: Myasthenia gravis (MG) is an autoimmune disease mediated by autoantibodies.. The important roles of circRNAs modified by m6A methylation have been reported in the pathogenesis of other autoimmune diseases, but remain unclear in MG. To address this point, we collected peripheral blood mononuclear cells from six MG patients and six healthy controls and performed m6A‐circRNA epitranscriptomic microarray and RNA sequencing.. Differentially m6A‐modified circRNAs and differentially expressed genes (DEGs) were analyzed.

Immunoprecipitation:

Article Title: Exploring the Roles of m6A-Modified circRNAs in Myasthenia Gravis Based on Multi-Omics Analysis.
Article Snippet: Myasthenia gravis (MG) is an autoimmune disease mediated by autoantibodies.. The important roles of circRNAs modified by m6A methylation have been reported in the pathogenesis of other autoimmune diseases, but remain unclear in MG. To address this point, we collected peripheral blood mononuclear cells from six MG patients and six healthy controls and performed m6A‐circRNA epitranscriptomic microarray and RNA sequencing.. Differentially m6A‐modified circRNAs and differentially expressed genes (DEGs) were analyzed.

Magnetic Beads:

Article Title: Exploring the Roles of m6A-Modified circRNAs in Myasthenia Gravis Based on Multi-Omics Analysis.
Article Snippet: Myasthenia gravis (MG) is an autoimmune disease mediated by autoantibodies.. The important roles of circRNAs modified by m6A methylation have been reported in the pathogenesis of other autoimmune diseases, but remain unclear in MG. To address this point, we collected peripheral blood mononuclear cells from six MG patients and six healthy controls and performed m6A‐circRNA epitranscriptomic microarray and RNA sequencing.. Differentially m6A‐modified circRNAs and differentially expressed genes (DEGs) were analyzed.

Labeling:

Article Title: Exploring the Roles of m6A-Modified circRNAs in Myasthenia Gravis Based on Multi-Omics Analysis.
Article Snippet: Myasthenia gravis (MG) is an autoimmune disease mediated by autoantibodies.. The important roles of circRNAs modified by m6A methylation have been reported in the pathogenesis of other autoimmune diseases, but remain unclear in MG. To address this point, we collected peripheral blood mononuclear cells from six MG patients and six healthy controls and performed m6A‐circRNA epitranscriptomic microarray and RNA sequencing.. Differentially m6A‐modified circRNAs and differentially expressed genes (DEGs) were analyzed.

Article Title: Genome-wide analysis of m6A-modified circRNAs in the mouse model of myocardial injury induced by obstructive sleep apnea.
Article Snippet: .. Following the addition of an equal quantity of calibration spike-in control RNA, the enriched “IP” and “Sup” RNAs were amplified independently and labeled with Cy3 (for “Sup”) and Cy5 (for “IP”) using the Arraystar Super RNA Labeling Kit. .. The purity and amount of total RNA sample were determined using the NanoDrop ND-1000 (pmol dye/μg cRNA).

Article Title: Circular RNA circDhx32 promotes cardiac inflammatory responses in mouse cardiac ischemia-reperfusion injury via binding to FOXO1 competed with AdipoR1.
Article Snippet: The “IP” and “Sup” RNAs were treated with RNase R to eliminate linear RNAs, and then, a RNeasy MinElute Cleanup Kit (cat. #74204, Qiagen, Dusseldorf, Germany) was used to purify the remaining RNA. .. Next, the enriched “IP” and “Sup” RNAs were labeled with Cy3 (for “Sup”) or Cy5 (for “IP”) and purified using a Super RNA Labeling Kit (Arraystar, MD, USA) and a RNeasy Mini Kit, respectively. .. The labeled RNAs were subsequently hybridized onto an Arraystar circRNA Epitranscriptomic Microarray (8 × 15 K, Arraystar).

Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis.
Article Snippet: .. The enriched circular RNAs were then amplified and transcribed into fluorescent cRNA using a random priming method provided by the Arraystar Super RNA Labeling Kit. .. The labeled cRNAs were subsequently hybridized onto the Arraystar Human circRNA Array V2 (8 × 15 K, Arraystar).

Article Title: Epitranscriptomic Analysis of the Ventral Hippocampus in a Mouse Model of Post-Traumatic Stress Disorder Following Deep Brain Stimulation Treatment of the Basolateral Amygdala.
Article Snippet: .. These “IP” and “Sup” RNA fractions were distinctly labeled with Cy5 and Cy3 fluorophores, respectively, employing the Arraystar Super RNA Labeling Kit to synthesize cRNAs. .. The amalgamated labeled cRNAs were then hybridized onto the Arraystar Mouse mRNA & lncRNA Epitranscriptomic Microarray (8 × 60K, Arraystar, Rockville, MD, USA).

Article Title: Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors
Article Snippet: The diagnosis of primary pancreatic neuroendocrine tumors (pNETs) presents significant challenges, and metastatic pancreatic neuroendocrine tumors are associated with high mortality.. Understanding the characteristics of these tumors, particularly the key molecules involved in metastasis, is essential.. To address this, we utilized mRNA expression data from human pNET and metastatic pancreatic tumor tissues available in the GEO database and integrated this data with bioinformatics analyses.

Article Title: Identification of the CeRNA axis of circ_0000006/miR-483-5p/KDM2B in the progression of aortic aneurysm to aorta dissection.
Article Snippet: After quantification using NanoDrop ND-1000 spectrophotometer, 10 μg sample was digested with 10 unit of Rnase R (Epicentre Biotechnologies, Beijing, China) at 37°C for 30 min to remove linear RNAs. .. Then the circRNA-enriched samples were amplified and transcribed into fluorescent circRNA using Arraystar Super RNA labeling kit (Arraystar, CA, USA). ..

Article Title: Circ_0088200 acts as a sponge for miR-127-5p to promote the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes
Article Snippet: .. The enriched circRNAs were then amplified and transcribed into fluorescent circRNAs using a random-priming method (Arraystar Super RNA Labeling Kit; Arraystar Inc.) according to the manufacturer’s instructions. .. Labeled circRNAs were hybridized to the Arraystar Human circRNA Microarray V2.0 (8 × 15K, Arraystar Inc.).

Control:

Article Title: Genome-wide analysis of m6A-modified circRNAs in the mouse model of myocardial injury induced by obstructive sleep apnea.
Article Snippet: .. Following the addition of an equal quantity of calibration spike-in control RNA, the enriched “IP” and “Sup” RNAs were amplified independently and labeled with Cy3 (for “Sup”) and Cy5 (for “IP”) using the Arraystar Super RNA Labeling Kit. .. The purity and amount of total RNA sample were determined using the NanoDrop ND-1000 (pmol dye/μg cRNA).

Amplification:

Article Title: Genome-wide analysis of m6A-modified circRNAs in the mouse model of myocardial injury induced by obstructive sleep apnea.
Article Snippet: .. Following the addition of an equal quantity of calibration spike-in control RNA, the enriched “IP” and “Sup” RNAs were amplified independently and labeled with Cy3 (for “Sup”) and Cy5 (for “IP”) using the Arraystar Super RNA Labeling Kit. .. The purity and amount of total RNA sample were determined using the NanoDrop ND-1000 (pmol dye/μg cRNA).

Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis.
Article Snippet: .. The enriched circular RNAs were then amplified and transcribed into fluorescent cRNA using a random priming method provided by the Arraystar Super RNA Labeling Kit. .. The labeled cRNAs were subsequently hybridized onto the Arraystar Human circRNA Array V2 (8 × 15 K, Arraystar).

Article Title: Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors
Article Snippet: The diagnosis of primary pancreatic neuroendocrine tumors (pNETs) presents significant challenges, and metastatic pancreatic neuroendocrine tumors are associated with high mortality.. Understanding the characteristics of these tumors, particularly the key molecules involved in metastasis, is essential.. To address this, we utilized mRNA expression data from human pNET and metastatic pancreatic tumor tissues available in the GEO database and integrated this data with bioinformatics analyses.

Article Title: Identification of the CeRNA axis of circ_0000006/miR-483-5p/KDM2B in the progression of aortic aneurysm to aorta dissection.
Article Snippet: After quantification using NanoDrop ND-1000 spectrophotometer, 10 μg sample was digested with 10 unit of Rnase R (Epicentre Biotechnologies, Beijing, China) at 37°C for 30 min to remove linear RNAs. .. Then the circRNA-enriched samples were amplified and transcribed into fluorescent circRNA using Arraystar Super RNA labeling kit (Arraystar, CA, USA). ..

Article Title: Circ_0088200 acts as a sponge for miR-127-5p to promote the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes
Article Snippet: .. The enriched circRNAs were then amplified and transcribed into fluorescent circRNAs using a random-priming method (Arraystar Super RNA Labeling Kit; Arraystar Inc.) according to the manufacturer’s instructions. .. Labeled circRNAs were hybridized to the Arraystar Human circRNA Microarray V2.0 (8 × 15K, Arraystar Inc.).

Purification:

Article Title: Circular RNA circDhx32 promotes cardiac inflammatory responses in mouse cardiac ischemia-reperfusion injury via binding to FOXO1 competed with AdipoR1.
Article Snippet: The “IP” and “Sup” RNAs were treated with RNase R to eliminate linear RNAs, and then, a RNeasy MinElute Cleanup Kit (cat. #74204, Qiagen, Dusseldorf, Germany) was used to purify the remaining RNA. .. Next, the enriched “IP” and “Sup” RNAs were labeled with Cy3 (for “Sup”) or Cy5 (for “IP”) and purified using a Super RNA Labeling Kit (Arraystar, MD, USA) and a RNeasy Mini Kit, respectively. .. The labeled RNAs were subsequently hybridized onto an Arraystar circRNA Epitranscriptomic Microarray (8 × 15 K, Arraystar).



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Differential <t>lncRNA</t> expression profiles between tissues of cisplatin-resistant and cisplatin-sensitive ovarian cancer. The lncRNA <t>microarray</t> demonstrated the differences between lncRNA expression in cisplatin-resistant and cisplatin-sensitive ovarian cancer through (A) hot-spot and (B) cluster map analysis. (C) Based on the association of the nearby coding genes, the differentially expressed lncRNAs were classified into certain types, including 312 natural antisense, 216 intronic antisense, 114 intron sense-overlapping, 673 intergenic, 201 exon sense-overlapping and 72 bidirectional lncRNA. lncRNA, long non-coding RNA.
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Differential lncRNA expression profiles between tissues of cisplatin-resistant and cisplatin-sensitive ovarian cancer. The lncRNA microarray demonstrated the differences between lncRNA expression in cisplatin-resistant and cisplatin-sensitive ovarian cancer through (A) hot-spot and (B) cluster map analysis. (C) Based on the association of the nearby coding genes, the differentially expressed lncRNAs were classified into certain types, including 312 natural antisense, 216 intronic antisense, 114 intron sense-overlapping, 673 intergenic, 201 exon sense-overlapping and 72 bidirectional lncRNA. lncRNA, long non-coding RNA.

Journal: Oncology Letters

Article Title: lncRNAs are novel biomarkers for differentiating between cisplatin-resistant and cisplatin-sensitive ovarian cancer

doi: 10.3892/ol.2018.8433

Figure Lengend Snippet: Differential lncRNA expression profiles between tissues of cisplatin-resistant and cisplatin-sensitive ovarian cancer. The lncRNA microarray demonstrated the differences between lncRNA expression in cisplatin-resistant and cisplatin-sensitive ovarian cancer through (A) hot-spot and (B) cluster map analysis. (C) Based on the association of the nearby coding genes, the differentially expressed lncRNAs were classified into certain types, including 312 natural antisense, 216 intronic antisense, 114 intron sense-overlapping, 673 intergenic, 201 exon sense-overlapping and 72 bidirectional lncRNA. lncRNA, long non-coding RNA.

Article Snippet: Following washing, the arrays were scanned using the Agilent lncRNA Microarray Scanner (Agilent Technologies, Inc.), and the Agilent Feature Extraction software version 11.0.1.1 (Agilent Technologies, Inc.) was used for microarray probe signal data collection.

Techniques: Expressing, Microarray