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legendplex cloud-based data analysis software  (Qognit Inc)

 
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    Qognit Inc legendplex cloud-based data analysis software
    Legendplex Cloud Based Data Analysis Software, supplied by Qognit Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/legendplex+cloud-based+data+analysis+software/legendplex+data+analysis+software+suite/pm40227192-244-4-9
    Average 90 stars, based on 1 article reviews
    legendplex cloud-based data analysis software - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Lipoyl deglutarylation by ABHD11 regulates mitochondrial and T cell metabolism.
    Article Snippet: Samples were thawed at room temperature and cytokine concentrations (IL-2, IL-4, IL-6, IL-10, IL-17A, IFNγ, TNF, soluble Fas, soluble FasL, granzyme A, granzyme B, perforin and granulysin) was quantified using the LEGENDplex Human CD8/NK V02 Panel (BioLegend, 741187), according to the manufacturer’s instructions. .. Data were collected using an Aurora flow cytometer (Cytek Biosciences) and analyzed using the LEGENDplex Data Analysis Software Suite (Qognit). ..

    Article Title: Immunogenicity and protective efficacy of an intranasal neuraminidase-based influenza vaccine with bacterial cell membrane-derived adjuvants.
    Article Snippet: .. Data were collected using an Attune flow cytometer (Thermo Fisher Scientific) and analyzed with LEGENDplex data analysis software (https://legendplex. qognit.com). .. Group comparisons were performed using one-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons in RStudio software (R version 4.3.3, RStudio Inc).

    Software:

    Article Title: Lipoyl deglutarylation by ABHD11 regulates mitochondrial and T cell metabolism.
    Article Snippet: Samples were thawed at room temperature and cytokine concentrations (IL-2, IL-4, IL-6, IL-10, IL-17A, IFNγ, TNF, soluble Fas, soluble FasL, granzyme A, granzyme B, perforin and granulysin) was quantified using the LEGENDplex Human CD8/NK V02 Panel (BioLegend, 741187), according to the manufacturer’s instructions. .. Data were collected using an Aurora flow cytometer (Cytek Biosciences) and analyzed using the LEGENDplex Data Analysis Software Suite (Qognit). ..

    Article Title: Immunogenicity and protective efficacy of an intranasal neuraminidase-based influenza vaccine with bacterial cell membrane-derived adjuvants.
    Article Snippet: .. Data were collected using an Attune flow cytometer (Thermo Fisher Scientific) and analyzed with LEGENDplex data analysis software (https://legendplex. qognit.com). .. Group comparisons were performed using one-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons in RStudio software (R version 4.3.3, RStudio Inc).

    Article Title: Antigen-dependent interplay of formulation, systemic innate responses, and antibody responses to multi-component replicon RNA vaccination
    Article Snippet: The acquired data were analyzed using FlowJo software (BD). .. LEGENDplex data were analyzed using LEGENDplex Data Analysis Software Suite (Qognit). ..

    Article Title: Platelet-Derived Microvesicles Modulate Cytokine and Lipid Mediator Profiles in THP-1 Monocytes and Macrophages.
    Article Snippet: Cytokine release was measured using flow cytometry with the LEGENDplex Human Inflammation Panel 1 (BioLegend), which allows the quantification of IL-1β, interferon (IFN)-α2, IFN-γ, tumor necrosis factor (TNF)-α, macrophage chemoattractant protein (MCP)-1, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, IL-23 and IL-33, following the manufacturer’s protocol. .. The results were analyzed using the cloud-based LEGENDplex data analysis software (Qognit). .. Samples were initially fixed in 2.5% glutaraldehyde (Sigma-Aldrich) in PBS at 4°C for 2 hours, followed by post-fixation in 1% osmium tetroxide (OsO4; Sigma-Aldrich) at 4°C for an additional 2 hours.

    Article Title: Nanomaterials Trigger Functional Anti-Tumoral Responses in Primary Human Immune Cells.
    Article Snippet: Cytokines were quantified by custom LEGENDplex (5-plex, BioLegend # 740 510) with the Attune NxT Flow Cytometer. .. Cytokine assays were analyzed using the LEGENDplex software Qognit (https://legendplex.qognit.com). .. Statistics: Statistical analyses were conducted using GraphPad Prism 9.5.

    Article Title: Giardia increases macrophage production of the anti-inflammatory cytokine interleukin-10 in response to lipopolysaccharide via macrophage galactose binding lectin.
    Article Snippet: .. The beads were acquired on Cytek Aurora (conventional mode) and analyzed with the LEGENDplex Data Analysis Software Suite from Qognit. ..

    Article Title: Cimicifuga racemosa extract Ze 450 shifts macrophage immunometabolism and attenuates pro-inflammatory signaling.
    Article Snippet: Cytokine secretion levels of macrophages were assessed with a Luminex Guava EasyCyte flow cytometer by exiting the samples with a 488 nm blue and a 635 nm red laser and capturing emission wavelengths of 532 nm and 660 nm when acquiring 5000 beads/sample. .. Data analysis was proceeded by using the online available LEGENDplex Data Analysis Software (Qognit, Inc., San Carlos, CA, USA). .. OCR and ECAR were examined using the XF96 Seahorse Metabolic Flux Analyzer from Seahorse Biosciences (Agilent Technologies, Waldbronn, Germany).

    other:

    Article Title: Cytokine, chemokine, and acute-phase protein profiles in plasma as correlative biomarkers of clinical outcomes for patients with COVID-19
    Article Snippet: Data were analyzed using the free online LEGENDplex cloud-based software suite ( https://legendplex.qognit.com/user/login?next=home ).



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    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by <t>LEGENDplex.</t> Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.
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    Image Search Results


    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Journal: Science immunology

    Article Title: Human LFA-1 governs T cell immune surveillance of the skin

    doi: 10.1126/sciimmunol.adz8360

    Figure Lengend Snippet: Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Article Snippet: Data were analyzed with LEGENDplex Cloud-based Data Analysis Software (Qognit).

    Techniques: Flow Cytometry, MANN-WHITNEY, Immunopeptidomics